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《CHINESE JOURNAL OF SPECTROSCOPY LABORATORY》 1999-03
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High Performance Liquid Chromatographic(HPLC) Determination of Internal Hormones in Wheat Seedlings

Z HN ZhiJia (Experiment Center,Hebei Normal University,No.265 Yuhuadong Lu,Shijiazhuang 050016,P.R.China) H AO ZhenFang (Department of Chemistry,Hebei Normal University,No.265 Yuhuadong Lu, Shijiazhuang 050016,P.R.China) Z HANG XiaoHui (Longyao Teach  
This paper describes the application of reversed\|phase HPLC to the determination of internal plant hormones including gibberellin(GA3)abscisic acid(ABA) and 3\|indoleacetic acid(IAA) in purified extracts of wheat seedlings,and also gives an extraction and purification procedure useful for the analysis of the hormones in plant tissue.A spherisorb C18 column was used and isocratic elution with MeOH\|1.8% HAc\|H2O(404020) at a flow rate of 2.5mL/min was performed with a UV detector at 254nm.
【CateGory Index】: O657.72
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Zhou Yang;Liu Lili;Liu Qian;Bai Wenjing;Wu Xinxin;Baoding Entry -Exit Inspection and Quarantine Bureau;;Determination of Gibberellin Residue in Bean Sprouts by HPLC-MS/MS[J];农药科学与管理;2015-06
2 FAN Guang-yu;LIU Ying-hui;ZHAO Zhi-hai;FENG Xiao-lei;WANG Xiao-ming;WANG Feng;LIU Xiao-jie;Zhangjiakou Academy of Agricultural Sciences;Hebei North University;;Detection of four plant hormones in foxtail millet leaf apex by HPLC[J];河北农业大学学报;2015-01
3 CAO Hui;CHEN Xiao zhen;ZHU Ying;WU Xiao-guang;WANG Li-na;ZHANG Xiao-bo;Zhejiang Institute of Quality Inspection Science;College of Chemical Engineering and Materials Science,Zhejiang University of Technology;;Determination of plant growth regulators in food by QuEChERS and ultra performance liquid chromatography-tandem mass spectrometry[J];化学试剂;2013-11
4 XU Sheng-jian;CAO Hui;CHEN Xiao-zhen;Zhejiang Institute of Quality Inspection Science;;Determination of Plant Growth Regulator Residues in Grains by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J];食品科学;2013-18
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6 SHI Yu-qin,CHEN Hong-yu,YIN Zhi-ping,WANG Si-si, LIANG Qiu-yuan,ZHAO Wen(College of Food Science and Technology,Agricultural University of Hebei,Engineering Technology Research Center for Agricultral Product processing of Hebei,Baoding 071001,China);Detection of residual gibberellic acid(GA_3) in vegetables and fruits by the SPE-HPLC method[J];农药学学报;2011-05
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8 ZHAO Ying-bo1,ZHOU Yan-ming1,XIN Xue1,2,WANG Yan-song3 (1.Analysis Test Center,Shenyang Agricultural University,Shenyang 110866,China; 2.Institute of Shenyang Grain and Oil Metering,Shenyang 110021,China; 3.Shenyang Product Quanlity Supervision and Inspection Institute,Shenyang 110022,China);Determination of Gibberellin Residue in Vegetables and Fruits by HPLC-MS/MS[J];食品科学;2011-06
9 JIN Xiao-Yun YU Mao-De XU Li WANG Xi-Ling ZHANG Qiong-Yu LIU Wei-Fen(College of Biotechnology,Southwest University,Chongqing 400716,China);Germination and Variations of Endogenous Phytohormone Content and SOD Activity of Mulberry Seeds Under Two Cultural Conditions[J];蚕业科学;2010-06
10 ZHANG Jun-Wei1,ZHOU Qing1,2 (1.The Key Laboratory for Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;2.School of Environment and Civil Engineering,Jiangnan University,Wuxi 214122,China);Effect of cerium on water metabolism in soybean seedling under UV-B radiation stress[J];中国生态农业学报;2009-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YAN Shi-Jie1, GUO Li-Wei2, WU Cai-E3, LIANG Li-Ya1, WANG Ji-Dong2, ZHANG Li-Na1 1(Food Science Department of Tianjin Agricultural University, Tianjin 300384) 2(College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801) 3(College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037);Simultaneous Determination of Three Kinds of Endogenous Hormones Content in Seeds of Post-harvest Yali Pear by High Performance Liquid Chromatography[J];分析化学;2010-06
2 TANG Li-juan1,WAN Yi-qun1,2, (1. Center of Analysis and Testing, Nanchang University, Nanchang 330047, China ;2. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China);Progress in Methods for Analyzing Plant Hormone[J];食品科学;2009-21
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4 WANG Jie,LI Min,ZHANG Yi-hui,YANG Sheng-chang*(School of Life Sciences,Xiamen University,Xiamen 361005,China);HPLC Analysis of ABA and GA3 in Reproductive Organs of Bruguiera gymnorrhiza[J];厦门大学学报(自然科学版);2008-05
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6 Li Weicheng1,3 Sheng Biyun2 Wang Shudong1Zhong Zheke1 Ding Yulong3 Liu Jing3(1.China National Bamboo Research Center,Hangzhou 310012,Zhejiang,China;2.Zhejiang Qingliangfeng National Nature Reserve Bureau,Hangzhou 311321,Zhejiang,China;3.Nanjing Forestry University,Nanjing 210037,Jiangsu,China);The Response of Moso Bamboo Seeds Germination to Temperature and Light[J];竹子研究汇刊;2007-04
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1 Zhou Yang;Liu Lili;Liu Qian;Bai Wenjing;Wu Xinxin;Baoding Entry -Exit Inspection and Quarantine Bureau;;Determination of Gibberellin Residue in Bean Sprouts by HPLC-MS/MS[J];农药科学与管理;2015-06
2 WANG Yuan;XING Li-jie;XIANG Xiao-li;Food Quality Supervision and Testing Center of Ministry of Agriculture,Xinjiang Academy of Agricultural Reclamation Science;;Determination of 8 Plant Growth Regulator Residues in Grape by UPLC-MS/MS[J];安徽农业科学;2015-15
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4 OUYANG Le-jun;SHA Yue-e;HUANG Zhen-chi;LI Li-mei;ZENG Fu-hua;Guangdong University of Petrochemical Technology;Zhanjiang Normal University;;Study on Difference of Callus of Eucalyptus urophylla × Eucalyptus grandis[J];中南林业科技大学学报;2014-12
5 GUO Hong-mei;LIANG Ju;WU Jiu-yun;JIA Qing-hua;Aierken;Ainiwaer;Reziwan;HU Xi-dan;;Determination of gibberellin residue in Thomsen seedless Grape by HPLC[J];中国园艺文摘;2014-11
6 Wang Yulin;Zeng Zhaohua;Liu Ying;College of Life Sciences,FAFU;Institute of Plant Protection,Fujian Academy of Agricultural Sciences;Liqing Bureau of Health;;Residual Decline of Gibberellin( GA_3) in Seawater[J];山东化工;2014-09
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8 LIU Xiaojia;TANG Xuedong;LIU Haiguang;WU Lin;ZHANG Zhidong;LI Yadong;College of Horticulture,Jilin Agricultural University;;The influence of soil amendments on cytokinin contents and their annual variations in the root zone of Vaccinium vitis-idaea[J];华南农业大学学报;2014-06
9 Yao Jie;Liu Chuande;Lu Zeqi;Wang Zhixin;Duan Xiaona;Xu Weihua;Yu Jianlei;Yantai Academy of Agricultural Sciences in Shandong Province;Institute of Plant Protection, Shandong Academy of Agricultural Science;;Dispelling Dynamic of Gibberellic Acid Residue in the Pear[J];中国农学通报;2014-12
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