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《Chinese Journal of Infection Control》 2008-03
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Analysis on change in drug-resistance and DNA polymorphism of Escherichia coli

HE Xiao-feng,CAO Jin-gui,ZHANG Hu,MA Wen-jie,WANG Min,WU Di,JIAO Li-qun(Air Force General Hospital,Beijing 100036,China)  
Objective To investigate the trend of antimicrobial resistance of Escherichia coli from 2001 to 2005,and analyse the DNA polymorphism.Methods Escherichia coli isolates from clinic were identified by VITEK-automated microbial identification system.Antimicrobial susceptibility test was carried out by Kirby-Bauer method.The genes of Escherichia coli were amplified by randomly amplified polymorphic DNA(RAPD)in 10 antimicrobial susceptible and 10 multidrug-resistant isolates.Results There was an elevated tendency of the resistance rates to other antimicrobial agents except imipenem and amoxicillin/clavulanic acid.10 susceptible strains were classified into 9 genotypes and 10 multidrug-resistant strains were classified into 3 genotypes by RAPD,6 of 10 multidrug-resistant strains were the same pattern.Conclusion The resistant rates of Escherichia coli to common antimicrobial agents are high and the resistant patterns are wide.There is correlation between antimicrobial resistant phenotypes and genotypes of Escherichia coli.
【CateGory Index】: R378.21
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