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Extraction of Genomic DNA from Vitis amurensis and Its RAPD Analysis

Wang Jun and He Puchao ( Institute of Spend Wild Economic Animal and Plant, GAAS, Jilin 132109; Department of Horticulture, Northwestern Agricultural University)  
The extracts of high quality genomic DNA was extracted from the frozen leaves of V. amurensis Rupr. (■,73064) and the fresh leaves of Xiangfei(V. vinifera L. )by modified CTAB, medium with high salts and SDS method respectively. The purity and production were different among those 3 methods. According to the comprehensive results, the method of GB was the best (A260/A280 = 1. 7, production 57 ng' mg~(-1) fresh leaves). ms method didn' t need the liquid N and phenol extraction, and the DNA length was similar to λ DNA (48 kb) .TIs isolated DNA could be used directly for RAPD analysis without sedimentation in cesium chloride gradient or column chromatography. It's really suitable for great amount samples for RAPD analysis.
【Fund】: 吉林省科委资助项目
【CateGory Index】: Q946.
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