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《Journal of Fruit Science》 2001-02
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The Determination of Carotenoids in Apple Peel by HPLC

Zhao Wenen 1,2 ,Zhang Jinqiang 1,Qiao Xiansheng 1,Yu Hong 1,and Han Yashan 3 ( 1Zhengzhou Fruit Research Institute,CAAS,Zhengzhou 450009; 2Chemical Engineering College,Zhengzhou University,Zhengzhou 450052; 3College of Food Sciences,China Agri  
A reversed phase high performance liquid chromatographic method was used to determine carotenoids in apple peel.Carotenoids were extracted from apple peel with acetone and petroleum ether.Using Novapak C 18 column( 4.6 mm ×150 mm),acetonitrile∶methanol∶methylene chloride (70∶20∶10) as mobile phase,carotenoids were eluted and monitored at 450 nm.It was shown that recoveries of zeaxanthin,lutein,lycopene and β carotene were 94.27 %~ 99.82 %, 84.56 %~ 89.41 %, 87.53% ~ 93.54 %,and 88.08% ~ 93.24 %.‘Fuji’ apple contained zeaxanthin ( 5.9 ×10 -3 μg/mm 2) and β carotene ( 5.4 × 10 -3 μg/mm 2 ) and Red Delicious apple only zeaxanthin ( 9.1 ×10 -3 μg/mm 2).
【CateGory Index】: S661.1
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