Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Fruit Science》 2001-06
Add to Favorite Get Latest Update

Optimization on RAPD Analysis System of Chinese Jujube(Ziziphus ju-juba Mill.)

Zhao Jin and Liu Mengjun (Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding071001)  
The optimized RAPD reaction condition of Chinese jujube was established after systematic study,i.e.in a total volume of25μL,containing100mmol/L KCl ,8mmol/L(NH 4 ) 2 SO 4 ,10mmol/L Tris-HCl (pH9.0),Np-40,2.0mmol/L Mg Cl 2 ,160μmol/L dNTP,15ng primer,30ng template DNA and1.5U Taq DNA polymerase.The amplification procedure of94℃4min,1cycle;94℃30s,36℃40s,72℃1min,50cycles;and72℃8min was suitable to Chinese jujube.The opti-mized RAPD analysis system was very similar to those obtained in other labs,with minor modification of the concentration of dNTP,primer,and Taq DNA poly merase.
【Fund】: 教育部留学回国人员基金;; 河北农业大学9816科技工程资助项目
【CateGory Index】: S665.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 LI Rui-huan et al(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100);Genomic DNA Extraction and RAPD System Optimization of Chinese Jujube[J];Journal of Anhui Agricultural Sciences;2007-17
2 CHEN Xiao-jun1,2,CHEN Yu-chao1,2,LI Miao1,2,GAN Xiao-yan1,2,LIU Ting-jun3,SONG Yu-xia 1,2(1.Key Lab of Agricultural Biotechnology of Ningxia,Yinchuan,Ningxia 750002;2.Academy of Agriculture and Forestry Sciences Agricultural Biotechnology Center,Yinchuan,Ningxia 750021;3.Ningxia Forestry Development Center of Economic Forestry,Yinchuan,Ningxia 750000);The RAPD Analysis of Zizyphus jujube Cultivars in Ningxia[J];Northern Horticulture;2010-01
3 ZHANG Xian~1,ZHANG Ying-jun~(1*),WU Wei-qun~2(1.Institute of Grassland Science,China Agricultural University,Beijing 100094,China;2.Yunnan Grassland and Feed Management Section,Kun Ming 650225,China);Optimization on RAPD Analytical System of White Clover[J];Acta Agrestia Sinica;2006-03
4 Cheng Qianding(College of Horticulture,Agricultural University of Hebei,Baoding 071000,P.R.China);Yang Liping(Yangtze Normal University);Li Rui(Agricultural University of Hebei);Morphological Traits and RAPD Identification of Lily Hybrids at Seedling Stage[J];Journal of Northeast Forestry University;2011-11
5 Ma Qinghua Xu Jiuru College of Biological and Technology, Beijing Forestry University, Beijing, 100083;Research Development on Molecular Markers in Chinese Jujube (Zizyphus jujuba Mill.)[J];Molecular Plant Breeding;2006-S2
6 Wang GuopingInstitute of Pomology, Shanxi Academy of Agricultural Science, Taigu, 030815;Possible Application of Protoplast Fusion Technology in Jujube Breeding[J];Molecular Plant Breeding;2008-03
7 QiaoYushan, Zhang Zhen, Fang Jinggui, ShenZhijun, and Zhao Mizhen (College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095 China; Research Institute of Horticulture, JiangsuA-cademy of Agricultural Sciences, Nanjing, Jiangsu 210014 China);Establishment of the Inter-simple Sequence Repeats Reaction System in Plum Germplasm[J];Journal of Fruit Science;2003-04
8 Qiao Yushan 1 ,Zhang Zhen 1 ,Fang Jinggui 1 ,and Guo Hong 2 ( 1 College of Horticulture,Nanjing Agricultural Uni versity,Nanjing,Jiangsu210095China;2 Research Institute of Horticulture,Jiangsu A-cademy of Agricultural Sciences,Nanjing,Jiangsu210014China);Optimal Reaction System of RAPD in Chinese Plum and Its Preliminary Use for Cul tivar Identification[J];Journal of Fruit Science;2003-06
9 ZHANG Shu-jing1,YANG Min-sheng1,LIANG Hai-yong1,WANG Wei-zheng2 (1 Forestry College,Agricultural University of Hebei,Baoding 071000,China;2 Agricultural Bureau of Jingxian County in Hengshui,Hengshui 053500,China);Optimization for SSR reaction system of grape[J];Hebei Journal of Forestry and Orchard Research;2008-03
10 LIU Xin1,ZHENG Wen-jing1,2,ZHANG Shao-bin1(1.Biological Science and Technology College,Shenyang Agricultural University,Shenyang 110161,China;2.Rice Research Institute,Liaoning Academy of Agricultural Science,Shenyang 110161,China);Optimization for SSR-PCR Reaction System of Rice[J];Hubei Agricultural Sciences;2009-07
Chinese Journal Full-text Database 7 Hits
1 Dai Silan; Chen Junyu; Li Wenbin(College of Landscape Architecture, BFU 100083)(Institute of Genetics, Academia Sinica, Beijing 100101);Selection of RAPD Reaction Conditions for Genus Dendranthema[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1996-01
2 Liu Mengjun(Research Center of Chinese Jujube, HAU, Baoding 071001)Shin Yong - UK ;Yae Byeong - WOO(National Horticultural Research Institute, Suwon 440310, Rep. of Korea);Factors Influencing RAPD Analysis in Genus Malus[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1998-04
3 Xia Ming Luan Fei-shi Li Jing-peng(Northeast Agricultural University, Harbin 150030);STUDY ON INFLUENCING FACTORS OF RAPD AND OPTIMIZATION OF RAPD EXPERIMENT CONDITIONS[J];;1999-02
4 Wang Yuejin1 Lamikara Olusola2(1 Department of Horticulture,Northwestern Agricultural University,Yangling,Shaanxi 712100;2 Center for Viticultural Science,Florida A & M Univessity,FL 32307 USA);Studies on Affecting Factors in the Analysis of Rard of Grapes[J];Journal of Agricultural Biotechnology;1997-04
5 Shen Xiang1Zheng Xueqin3Ren Xiaolin2Li Jiarui2 (1Department of Horticultural, Shandong Agricultural University,271018) (2Department of Horticultural, Northwest Agricultural University,712100) (3National Biotechnology Lab for Tropical Crops,571737);OPTIMIZATOIN OF STONE FRUITS GENOMIC DNAISOLATION AND RAPD CONDITIONS[J];Journal of Shandong Agricultural University;1999-02
6 FAN Yuan hong 1 CAI Qing 1 SU Bing 2 ZHANG Ya ping 2 (1 Sugarcane Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan,Yunnan Province,661000,China 2 Laboratory of Cellular and Molecular Evolution,Kunming Institute;Effects of DNA extraction and purification processes on the RAPD results of six Saccharinae species[J];SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES;1999-01
7 Chen Daming; Zhang Shanglong ; Jin Yongfeng (Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029, China);A method for genomic DNA preparation of woody fruit crops[J];Journal of Zhejiang Agricultural University;1997-06
Chinese Journal Full-text Database 10 Hits
1 NIU Yong_dong 1, XU Li_yan 2, LI En_min 1(1. Department of Biochemistry & Molecular Biology; 2. Insititue of Oncologic Pathology,Medical College of Shantou University, Shantou 515031 , China);CONSTRUCTION AND IDENTIFICATION OF THE BAIT PLASMIDS pHis-3NF AND pLacZ-3NF FOR YEAST ONE-HYBRID SYSTEM[J];Carcinogenesis,Teratogenesis and Mutagenesis;2003-01
2 OUYANG Song_ying 1 ,XU Shou_shui 2, ,SHEN Wen_lu 2 ,et al (1.Faculty of Military Veterinary Medicine,The Quartermaster University of PLA,Changchun130062,China;2.The Second Affiliated Hospital of Shantou University Medical College,Shantou515041,China);Construction of PTEN Gene Expression Vector and Its Expression in Glioma Cell Lines U_(251)[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-04
3 LI Tao,SHI Nian,WU You-tong,et al (Department of Health Toxicoliogy,Tongji Medical College of Huazhong University of Science and Technology,Wuhan430030,China);Study on the Effect of Bcl-2 in Deltamethrin Induced Neural Cells Apoptosis[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-05
4 Yang Liping,Zhang Yusheng,Gao Yundong,Zhong Jifeng,Hou Minghai & Jiang Wenxue (Animal Science and Veterinary Medicine Institute,Shandong Academy of Agricultural Sciences,Jinan 250100);Optimization of RAPD Reaction System for Rabbit Genetic Analysis[J];SHANDONG AGRICULTURAL SCIENCES;1999-05
5 WEI Guo-qin1,2,DAI Hong-yi1*,SUN Yu-gang2,LIANG Mei-xia1,AN Miao2 (1.College of Horticulture,Qingdao Agricultural University,Qingdao 266109,China;2.Shandong Institute of Pomology,Taian 271000,China);Research on Propagation in Vitro of Quince[J];Shandong Agricultural Sciences;2010-01
6 LIU Yuan-yuan,ZHANG Chun-qing,WU Cheng-lai(Agronomy College of Shandong Agricultural University,Taian 271018,China);Establishment of System for Transforming Antisence LeJA2 Gene into Tomato[J];Shandong Agricultural Sciences;2010-02
7 SHI Dong-yan1,CHENG Zai-quan2 (1.Biology Department of Heze University,Heze 274015,China;2.Biotechnology and Genetic Germplasm Institute,Yunnan Academy of Agricultural Sciences,Kunming 650223,China);Cloning and analysis on sequence of metallothionein gene from Yuanjiang O. rifupogon[J];Journal of Anhui University(Natural Sciences);2009-03
8 Ju Yudong et al.(Fujian Academy of Agricultural Sciences Sugarcane Research Institue,363005);Cloning and Sequence Analysis of a MADS-box Homologous Gene from Narcissus tazetta var. chinensis[J];Anhui Agricultural Science Bulletin;2008-21
9 Zhang Baoqiong1,2 et al.(1 Flower Research Insistute Of Yunnan Academy Agriculture Sciense,Kunming,650205;2 Yunnan Agriculture University,Kunming 650201;3 Southwest Forestry College,Kunming 650224);Study on Selecting of Transgenic Gerbera Plants with kanamycin[J];Anhui Agricultural Science Bulletin;2009-03
10 Zhang Xia et al.(College of Horticulture and Landscape,Southwest University,Chongqing 400715,China;Institute of Ornamental Research,Southwest University,Chongqing 400716,China);Study on the Extraction Methods and Technological Conditions for Volatile Oil in the Leaves of Michelia alba DC[J];Anhui Agricultural Science Bulletin;2011-03
China Proceedings of conference Full-text Database 10 Hits
1 TIAN Yong-qiang,ZHANG Rong-yi,ZHU Zhong-yuan.(College of Environment and Plant Protection,Hainan University,Haikou 571737,Hainan,P.R.China);Establishment and Optimization of the Line Probe Assay(LiPA) for the Human Papillomavirus[A];[C];2009
2 ;HIV-1跨膜蛋白gp41的截短表达及其免疫反应性[A];[C];2011
3 Zhu Mei-cai,Zhan Zhi,Wang Ya-jing,et al.(Department of Clinical Experiment,Air Force General Hospital,Beijing 100036,China);Expression of Erythrina trypsin Inhibitor variant rserETI in E.coli and application in purification of recombinant tPA[A];[C];2009
4 YIN Jun REN Jiang-ping LI Zhi-gang LI Lei ZHOU Su-mei WEI Li SONG Li (National Engineering Research For Wheat,Henan Aricultural University,Zhengzhou 450002,China);The study on transgenic wheat with the characteristic of pre-harvest sprouting resistance[A];[C];2007
5 WANG Ying ZHUANG Nan-sheng~* GAO He-qong HUANG Dong-yi (College of Agronomy,South China University of Tropical Agriculture,Danzhou,Hainan 571737,China);ISSR analysis for sugarcane germplasm[A];[C];2007
6 ZHAO Jie, LIU Chuwu (College of Fishery, Guangdong Ocean University, Zhanjiang 524025, Guangdong, China);Establishment of AFLP Experimental System in Snappers[A];[C];2010
7 WU Jie~1,ZHOU Yu~2,ZHANG Xue-mei~2,YAN Wen-zhao~1 (1.Institute of Biotechnology and Nuclear Technology,Sichuan Academy of Agriculture Sciences,Chengdu 610066,China;2.Chengdu Institute of biological products,Chengdu,610023);Transformation of heat shock protein gene(HspB-C)of helicobacter pylori into sweet potato varieties[A];[C];2009
8 Fang Jinggui, Qiao Yushan, Gao Zhihong, Wang Sanhong (Department of Horticulture, Nanjing Agricultural University, Nanjing 210095);The Utilization of DNA Presen'ation in the Conservationof Fruit Germplasm Resources[A];[C];2000
9 Huang Daiqing, Lu Liuxin ( College of Horticulture , Fujian Agriculture and Forestry University, Fuzhou, 350002; College of Bio - engineering, Fujian Normal University, Fuzhou , 350007);PCR-based Cloning and Analysis of NBS-LRR Class Resistance Gene Analogs in Five Fruit Trees[A];[C];2002
10 Liu Chengming and Mei Mantong ( College of Horticulture , Genetic Engineering Laboratory, College of Life Scicence , South China Agricultural University, Guangzhou 510642);Researches on DNA isolation and Optimal Parameters of RAPD in Some Sapindaceae Fruit Trees[A];[C];2002
Chinese Journal Full-text Database 10 Hits
1 Chen Shuqin et al (College of Forestry and Horticulture,Henan Agricultural University,Zhengzhou,Henan 450002);Detection of Removal Rate of Several Detergents to Pesticide Residues on Summer Cabbage[J];Journal of Anhui Agricultural Sciences;2004-02
2 Jing Zuoliang et al (Tianjin University of Science and Technology,Tianjin 300222);Study on the Measurement of the Pesticide Residue in Crop[J];Journal of Anhui Agricultural Sciences;2004-05
3 CHEN Zhi-qun et al (College of Agro-forestry Sciences,Linyi Teacher's University,Linyi,Shandong 276003);Technique of Tissue Culture and Plantlet Regeneration of Fraxinus velutina[J];Journal of Anhui Agricultural Sciences;2006-03
4 YAN Yi- zi et al (Northeast Forestry University,Harbin,Heilongjiang 150040);RAPD Analysis of Germplasm Resources of Platycodon grandiflorum DC[J];Journal of Anhui Agricultural Sciences;2006-16
5 QI Ye-feng et al (Agricultural University of Hebei,Baoding,Hebei 071001);Analysis on International Research Literature on the Genus Ziziphus Mill.[J];Journal of Anhui Agricultural Sciences;2008-22
6 QI Xiang-ying et al (College of Life Sciences,Yan'an University,Shaanxi Engineering & Technological Research Center of Conversation & Utilization of Biological Resources,Yan'an,Shaanxi 716000);Chromosome Number and Karyotype Analysis of Chinese Date Goutouzao[J];Journal of Anhui Agricultural Sciences;2008-26
7 GAO Dao-xia et al(Zhangshu Institute for Drug Control of Jiangxi Province,Zhangshu,Jiangxi 330000);RAPD Analysis of Different Cultivars of Jiangxi Citrus aurantium Linn.[J];Journal of Anhui Agricultural Sciences;2009-08
8 ZHU Ke-ke et al (Key Laboratory of Molecular Biology and Genetic Engineering of Jiangxi Province,Nanchang University,Nanchang,Jiangxi 330031);Molecular Mechanism of the Coordinated Expression of Ribosomal Protein Genes[J];Journal of Anhui Agricultural Sciences;2009-35
9 Zhang Tie Peng Chao Zhou Qun (Department of Horticulture,Anhui Agricultural University,Hefei 230036);Effects of GA 3 Treatment on Blooming and Fruiting of Japanese Apricot[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1997-04
10 Yang Jun 1 Xu Kai 1 Yan Chunguo 1 Shen Caodong 2 (1.Department of Horticulture,Anhui Agricultural University,Hefei 230036; 2.Department of Horticulture,Zhejiang Agricultural University);Studies on Isozyme of Peroxidase in Some Plum Varieties[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1998-04
China Proceedings of conference Full-text Database 1 Hits
1 Liu Mengjun (Research Center of Chinese Jujube, Hebei Aguicultural University,Baoding 071001);The Application of RAPD Technique on Germplasm Identification of Chinese Jujube and Acid Jujube[A];[C];1995
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 REN Hai-xia,GONG Zhi-yuan,LI Jin,REN Peng-fei,YAO Qiang,QU Ling (Institute of Soil and Fertilizer of Shandong Academy of Agricultural Sciences,Jinan 250100,China);Studies of Optimum RAPD-PCR Conditions for Pleurotus ostreatus[J];Shandong Agricultural Sciences;2009-03
2 Lv Yanzhen et al.(School of Life Science,Huaibei Normal University,Huaibei 235000,China;Anhui Key Laboratory of Plant Resources and Biology,Huaibei 235000,China);Optimization of ISSR Reaction system in Carya cathayensis[J];Anhui Agricultural Science Bulletin;2011-07
3 LIU Feng et al(Zhejiang Normal University,Chemistry and Life Science,Jinhua,Zhejiang 321004);Research on the Orthogonal Optimization of ISSR-PCR Reaction System of Madagascar Periwinkle[J];Journal of Anhui Agricultural Sciences;2010-05
4 CHU Jian-qing et al (College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095);Research on the Different Eelectrophoretic Detection of Plum RAPD Amplified Fragments and Analysis of Its Sequence[J];Journal of Anhui Agricultural Sciences;2010-25
5 WANG Lan et al(College of Life Sciences,Sichuan University,Chengdu,Sichuan 610064);Establishment and Optimization of ISSR-PCR Amplification System for Ligusticum chuanxiong Hort[J];Journal of Anhui Agricultural Sciences;2011-23
6 SUN Li-na 1,2 XI Meng-li 1 XU Jin 1 WU Zhu-hua 1 SHI Ji-sen 1 (1 The Key Laboratory of Forest Genetic and Biotechnology,Ministry of Education,Nanjing Forestry University,Nanjing Jiangsu 210037;2 Guangxi Forestry Research Institute);Research of ISSR Identifying Technology of Lily Radiation Mutants[J];Modern Agricultural Sciences and Technology;2011-01
7 SUN Wen-wen1,SUN Jun1,ZHOU Jun-yong2,SUN Qi-bao2(1 School of Horticulture,Anhui Agricultural University,Hefei 230036; 2 Horticulture Research Institute,Anhui Academy of Agriculture Science,Hefei 230031);Establishment of inter-simple sequence repeat(ISSR) reaction system and construction of fingerprint in Ziziphus jujuba genus[J];Journal of Anhui Agricultural University;2011-06
8 WU Bo1,2,GAO Dan3,PAN Chao-mei1,ZHANG Shou-wen3(1.College of Pharmacy,Guangzhou University of Chinese Medicine,Guangzhou 510006; 2.Chinese Medicine Germplasm Resource Engineering Technology Research Center,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004; 3.College of Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004);Comparation on different methods on genomic DNA and RNA extraction from young leaves of Evodia rutaecarpa[J];Journal of Anhui Agricultural University;2012-01
9 SHI Rui (Horticulture Department,Yanbian Aqricultural University,Lonjing,JiLin 133400);Comparison of Different Genomic DNA Extraction Methods Among Part of Pears[J];Northern Horticulture;2007-04
10 JIANG Xiang-Hui1,2,SHE Chao-Wen1,2,ZHANG Ling-Ling1,WU Xian-Jin1,2(1.Department of Bioengineering,Huaihua College,Huihua,Hunan 418008,China;2.Key Laboratory of Huaihua for Breeding and Bioprocess Technology,Huihua,Hunan 418008,China);Orthogonal Optimization of RAPD System for Pepper Varieties in Hunan Province[J];Northern Horticulture;2007-09
China Proceedings of conference Full-text Database 6 Hits
1 Qi Jing Shen Lianying Mao Yongmin Song Renping (College of Horticulture,Agriculture University of Hebe,Baoding 071001,China);Research advances on molecular markers in Chinese Jujube[A];[C];2005
2 XUE Hua-bo~(1,2),QIAO Yu-Shan~1,ZHANG Zhen~(1*),CAO Shang-Yin~2,GUO Jun-Ying~2,and ZHANG Hui-Rong~3 (1 College of Horticulture,Nanjing Agricultural University;Nanjing 210095,China;2 Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;3 Henan Institute of Science and Technology;Xinxiang;Henan 453003;China);Development of a Microsatellite Marker among Two Varieties of Prunus salicina Lindl[A];[C];2009
3 ZHENG Yi-qi~(1,2) WANG Zhi-yong~2 GUO Hai-lin~2 XUE Dan-dan~2 LIU Jian-xiu~(2*) (1.Department of Horticulture Nanjing Agricultural University Nanjing 210095 China; 2.Institute of Botany Jiangsu Province and Chinese Academy of Sciences Nanjing 210014 China);Optimization of SRAP-PCR System on centipedegrass Using Orthogonal Design and Selection of Primers[A];[C];2007
4 ;Research Progress on Molecular Breeding of Chinese Jujube[A];[C];2009
5 Zhu Jian Lin Bo-De Xiong Fang Ying Zheng-He Lu Qi-Quan Xie Bao-Gui (College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002,China);OPTIMIZATION OF SRAP-PCR AMPLIFICATION ON LENTINULA EDODES[A];[C];2006
6 LI Ya-nan YUAN Ying-ying LIANG Yun FENG Hui-ying XU Lei-feng YUAN Su-xia LIU Chun MING Jun (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Science,Beijing 100081);Advances in Hybridization Identification of Lilium[A];[C];2013
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Fu Junhua Li Lancheng Liu Xinzhi Peng Zebin Huang Changling(Institute of Crop Breeding and Cultivation, CAAS, Beijing, 100081);Studies on the Protocol Optimum and Preliminary Applification RAPD in Maize[J];Acta Agronomica Sinica;1997-01
2 Hui Dongwei; Zhuang Bingchang;Chen Shouyi(Institute of Genetics,Academia Sinica,Beijing 100101)(Institute of Soybean Research, Jiling Academy of Agriculture Sciences Gongzhuling 136100);Phylogeny of Genus Glycine Reconstructed by RAPD Fingerprinting[J];ACTA GENETICA SINICA;1996-06
3 Chen Hong, Zhu Li huang, Xu Ji chen and Chen Mei ling (Institute of Genetics, Academia Sinica, Beijing 100101);CONSTRUCTION OF RICE RAPD MOLECULAR LINKAGE MAP[J];Acta Botanica Sinica;1995-09
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved