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《Journal of Fruit Science》 2001-06
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Optimization on RAPD Analysis System of Chinese Jujube(Ziziphus ju-juba Mill.)

Zhao Jin and Liu Mengjun (Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding071001)  
The optimized RAPD reaction condition of Chinese jujube was established after systematic study,i.e.in a total volume of25μL,containing100mmol/L KCl ,8mmol/L(NH 4 ) 2 SO 4 ,10mmol/L Tris-HCl (pH9.0),Np-40,2.0mmol/L Mg Cl 2 ,160μmol/L dNTP,15ng primer,30ng template DNA and1.5U Taq DNA polymerase.The amplification procedure of94℃4min,1cycle;94℃30s,36℃40s,72℃1min,50cycles;and72℃8min was suitable to Chinese jujube.The opti-mized RAPD analysis system was very similar to those obtained in other labs,with minor modification of the concentration of dNTP,primer,and Taq DNA poly merase.
【Fund】: 教育部留学回国人员基金;; 河北农业大学9816科技工程资助项目
【CateGory Index】: S665.1
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