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《Journal of Fruit Science》 2003-04
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Establishment of the Inter-simple Sequence Repeats Reaction System in Plum Germplasm

QiaoYushan, Zhang Zhen, Fang Jinggui, ShenZhijun, and Zhao Mizhen (College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095 China; Research Institute of Horticulture, JiangsuA-cademy of Agricultural Sciences, Nanjing, Jiangsu 210014 China)  
The optimal reaction of ISSR (Inter-simple Sequence Repeats) in Prunus salicina cv. Niuxinli was studied with primer 5'-(AC)9A-3'. The results showed that the optimum concentration of five important components i.e. Taq DNA poly-merase, Mg2+, primer, template DNA and dNTPs in 25 μX reaction system of ISSR were 0.3U, 1.5 mmol/L, 0.2 μmol/L, 20 ng and 0.16 mmol/L, respectively. The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5'-(AC)9T-3'and 5'-(AC)9C-3', respectively. The identification rate of plum cultivars and species or variety was 100%.
【CateGory Index】: S662.3
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