Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Fruit Science》 2003-04
Add to Favorite Get Latest Update

Research on Using the Segment of Cotolydon as Explant in vitro for Regeneration of Seed Watermelon

Shi Jiang and Le Jinhua (South China University for Tropical Agriculture ,Haikou, Hainan 571101 China; Bioengineering College , Shihezi University ,Shihezi, Xinjiang 832003 China)  
Seed watermelon ( Citrullus lanatus ssp. vulgaris convar. megalaspermus Lin et Chao.) is the one convar. of ssp. vulgaris. It is one of the well-known, high quality seed-use watermelon. This paper reports how the factors affecting tissue culture as we aims to establish a quick, high frequency regeneration system of genetic translation for seed watermelon. The results indicated that the basal and middle segments of cotyledon of the young plants of 1 to 6 days age were the best appropriate explants. The combination of 6-BA 2 mg/L+IBA 0.1 mg/L was the optimum medium for differentiation of buds. The adventitious buds were found at the basal parts of central or lateral veins. The adventitious shoot elongation was the best on MS supplemented with 0.2 mg/L KT. On MS plus IAA 0.5 mg/L medium, the shoots stretched better and the rooting rates was 80%. This experiment has explored the suitable culture medium for bud inducing, shoot elongating and rooting which fit cotyledon. Through the test and verification at the National Key Biotechnology Laboratory for Tropical Crops, the results were proved to be steady and reliable, it is believed that the research will be useful for the seed watermelon tissue culture, utiliazation of so-.matic clonal variation and its genetic transformation.
【CateGory Index】: S651
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 YU Yi-he et al(Horticulture Plant Tissue Culture Institute of College of Landscape,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Study on Pumpkin Culture in Vitro and Regeneration[J];Journal of Anhui Agricultural Sciences;2006-20
2 FANG Li,REN Hai-ying,RU Shui-jiang,WANG Han-rong*(Institute of plant protection and microbiology,Zhjiang Academy of Agricultural Sciences,Hangzhou 310021,China);Agrobacterum tumefaciens-mediated genetic transformation in melon[J];Acta Agriculturae Zhejiangensis;2009-03
Chinese Journal Full-text Database 3 Hits
1 Hao Lixin \ \ \ Wang Huaiming (Beijing Vegetable Research Center,Beijing\ 100081);A Study on Building up the Regenerate System of Watermelon[J];ACTA AGRICULTURAE BOREALL—SINICA;1998-03
2 WANG Hui Zhong ZHOU Xiao Yun ZHANG Xi Ning (Biology Department, Hangzhou Normal College, Hangzhou 310036) (Zhejiang University of Technology, Hangzhou 330027);STUDIES ON THE TRANSFORMATION OF CITRULLUS VULGARIS Schrad AND REGENERATION OF TRANSGENIC PLANTS[J];Acta Biologiae Experimentalis Sinica;1997-04
3 Wang Chunxial,Jian Zhiying2,Liu Yu2,and Zou Qil (1Shandong Agricultural, Taian 271018;2Shanghai Institute of Plant Physiology,Academia Sinica, Shanghai 200032);Tissue Culture from Cotyledon of Watermelon 'Jing Xin No.1'[J];Acta Horticulturae Sinica;1996-04
Chinese Journal Full-text Database 10 Hits
1 YU Yi-he et al(Horticulture Plant Tissue Culture Institute of College of Landscape,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Study on Pumpkin Culture in Vitro and Regeneration[J];Journal of Anhui Agricultural Sciences;2006-20
2 ZHAO Cai-ping,WANG Rong-hua,ZHANG Zhi-ping,WANG Liang-ju(College of Horticulture,Nanjing Agricultural University,Nanjing 210095);Optimized system for plant regeneration of watermelon[J];Journal of Anhui Agricultural University;2009-02
3 LI Jing1,LI Huan Xiu2,LI Min3(1.Horticulture Department,Xichang Agricultural College,Xichang 625013 China;2.College of Forestry and Horticulture,Sichuan Agricultural Univercity,ya'an 625014,China;3.Heze College,Heze,Shandong 525013,China);The System of In Vitro Culture and Shoot Regeneration from Cotyledon of Balsam Pear of ‘Cuifei'[J];Northern Horticulture;2007-10
4 Li Jing, Li Huanxiu, Li Min;Research Progress of Cotyledon in Vitro Culture of Gourd Vegetable Crops[J];Journal of Changjiang Vegetables;2004-07
5 Lu Yongfen, Liu Jianghai, Xie Shunzan, Xie Rongrong;Studies on the in Vitro Culture of Brassica Pekinenis×B.olercea[J];Journal of Changjiang Vegetables;2004-07
6 CHENG Yong-qiang,LI Xi-huan,WANG Jing-hua,XU Jin-ru,CHENG Lin,DUAN Hui-jun(Key Laboratory of Crop Germplasm Resources of Hebei,Agricultural University of Hebei,Baoding 071001,China);Study on tissue culture of watermelon 'Jing Xin No.1' male parent[J];Journal of Agricultural University of Hebei;2009-02
7 ZhangAijia ,FangJinqiang ,QiuJinhai,WuDeyi (PutianCityBiologicalEngineeringResearchInstitute ,FujianProv.35 110 0China);A Study on Tissue Culture for Rapid Multiplicati on of Triploid Watermelon[J];Fujian Fruits;2003-03
8 Ai Chengxiang Liu Zhixin State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 571101;Advances and Prospects of Watermelon Genetic Transformation[J];Molecular Plant Breeding;2004-03
9 LU Yong-fen,MA Chun-mei,HUANG Rui-ming,XIE Xiao-kai,LIU Jiang-hai,LU Hai-qiang (Shantou Baisha Institute of Vegetable seeds Propagation,Shantou 515800,China);Investigation on factors influencing the cultural effects of difference explants from Brassica pekinenis×B.olercea[J];Guangdong Agricultural Sciences;2006-10
10 Liu Liwang1,Chen Chongshun2,Chen Feng1, and Zhao Liping1(1National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing,Jiangsu 210095 China; 2College of Life Sciences, Nanjing Normal University, Nanjing,Jiangsu 210097 China);Advances of Biotechnology Utilization in Genetics and Breeding of Watermelon (Citrullus lanatus)[J];Journal of Fruit Science;2004-05
China Proceedings of conference Full-text Database 1 Hits
1 He Xiaoming, Xie Dasen, and Peng Qingwu ( Vegetable Research Institute, Guangdong Academy of Agricultural Sciences , Guangzhou 510640);Effects of Culture Factors on Plant Regeneration of Chieh-qua[A];[C];2002
Chinese Journal Full-text Database 10 Hits
1 SU Shaokun,LIU Hongyu,QIN Zhiwei(Horticulture College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Establishment of genetic transformation system by Agrobacterium-mediated parthenocarpy gene of cucumber[J];Journal of Northeast Agricultural University;2006-03
2 Huang Yonghong1 Mei Mei2 Zeng Jiwu1 Zhou Birong1 Wu Yuanli1 Yi Ganjun1 1 Fruit Research Institute, Guangdong Academy of Agricultural Science, Guangzhou, 510640; 2 Jiaozhou Bureau of Agriculture, Qingdao, 266300;The Factors Influencing on Transformation of Cucumis melon Mediated by Agrobacterium[J];Molecular Plant Breeding;2007-03
3 ZHANG Zhi-zhong,WU Jing-hua, LV Liu-xin (College of Horticulture , College of Life Sciencs ,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002 China);Studies on Agrobacterium tumefaciens mediated genetic transformation of watermelon[J];Journal of Fruit Science;2005-02
4 LIU Li-feng,GU Qin-sheng,HU Jing-ang,YU Zheng-wang,LIU Jun-pu,PENG Bin,LI Li(Zhengzhou Fruit Research Institute,CAAS,Zhengzhou,Henan 450009 China);Transformation of watermelon with Zucchini yellow mosaic virus coat protein gene[J];Journal of Fruit Science;2007-04
5 CAO Li-xian1,ZHAO Li2,TANG Yu-li3,ZHU Cheng4 (1. Jiaxing College Medical College, Jiaxing 314001, China;2. Lishui Agricultural School, Zhejiang Lishui 323000, China;3. Zhejiang Hangzhou Botany Garden, Zhejiang Hangzhou 310013, China; 4. College of;Stimulation effect of silver nitrate on shoot regeneration in cotyledon tissue culture of cucumber[J];Journal of Gansu Agricultural University;2001-02
6 SUN Yu-hong1) HAN Chang-lei2) ZHANG Chun-yu2) XIAO Yong2) ZENG Hong-xia1) PENG Jin-guang1) DAI Shu-tao2) MENG Jin-ling2)(1) Wuhan Institute of Agricultural Sciences,Wuhan 430051,China;2)National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China);Transgenic Watermelon Plants Obtained by Agrobacterium tumefaciens-mediated Transformation[J];Journal of Huazhong Agricultural University;2007-05
7 MEI Qian,ZHANG Xing-guo *(Department of Horticulture, Southwest Agricultural University, Chongqing 400716, China);STUDIES ON TISSUE CULTURE OF CUCUMBER[J];Journal of Southwest Agricultural University;2002-03
8 ZOU Jian1, SONG Ming1, TANG Qing-lin1, ZHANG Zhong-ling2, LIU Hong-yu2, ZHOU Hong2(1. Department of Horticulture, Southwest Agricultural University, Chongqing 400716, China; 2. Chongqing Bureau of Agriculture, Chongqing 400010, China);PRELIMINARY STUDY OF IN VITRO CULTURE OF COTYLEDON EXPLANTS OF ORNAMENTAL CUSHAW (CUCURBITA MOSCHATA)[J];Journal of Southwest Agricultural University;2003-04
9 Wang Chunxial,Jian Zhiying2,Liu Yu2,and Zou Qil (1Shandong Agricultural, Taian 271018;2Shanghai Institute of Plant Physiology,Academia Sinica, Shanghai 200032);Tissue Culture from Cotyledon of Watermelon 'Jing Xin No.1'[J];Acta Horticulturae Sinica;1996-04
10 Hou Aiju,Zhu Yanming,Yang Aifu,and Zhang Binbin (Plant Bioengineering Laboratory,Life Science Institute,North East Agriculture University,Harbin 150030,China);Main Factors Influencing the Frequency of Direct Organogenesis of Cucumber in Vitro[J];Acta Horticulturae Sinica;2003-01
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 LI Chang-sheng,ZHAO Chuan-zhi,LI Ai-qin,XIA Han(High-Tech Research Center,Shandong Academy of Agricultural Sciences/ Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture/Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province,Jinan 250100,China);System Establishment of Tisue Culture in Vitro and Transgene Technology for Cucurbita ficifolia B.[J];Shandong Agricultural Sciences;2009-07
2 ZOU Keqin ZHANG Yongjun LOU Jidong (College of Life Science,China Jiliang University,Hangzhou Zhejiang 310018);Progress of Cucurbita moschata Duch.[J];Journal of Changjiang Vegetables;2008-08
3 BAI Jing, QU Shu-ping, CUI Chong-shi(College of Horticulture Northeast Agricultural University, Harbin, Heilongjiang, 150030, China);Advances in tissue culture and genetic transformation of cucurbit vegetables[J];China Cucurbits and Vegetables;2008-04
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Sun Lihua She Jianming Lu Xuefeng (Institute of Crop Genetics and Physiology,Jiangsu Academy of Agricultural Sciences,Nanjing);Selection of Mutants of Xanthomonas oryzae by Tissue Culture in Rice——Ⅰ. In vitro Induction and Screening of Mutants Resistant to Xanthomonas oryzae Dowson from Callus Culture in Rice[J];Acta Genetica Sinica;1986-03
2 Ling Dinghou (South China Institute of Botany,Academia Sinica,Guangzhou)P.Vidhyaseharan E.S.Borromeo F.J.Zapata Miao Donghua (International Rice Research Institute,Manila,Philippines);In vitro Screening of Rice Germplasm for Resistance to Brown Spot Disease Using Phytotoxin[J];Acta Genetica Sinica;1986-03
3 Xu Zhi-hong;Wei Zhi-ming;Liu Gui-yun Laboratory of Cell Physiology, Shanghai Institute of Plant Physiology Academia Sinica;IN VITRO CLONAL PROPAGATION OF TRIPLOID SEEDLESS WATER MELON (CITRULLUS VULGARIS SCHRAD.)[J];;1979-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved