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《Journal of Fruit Science》 2003-04
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Research on Using the Segment of Cotolydon as Explant in vitro for Regeneration of Seed Watermelon

Shi Jiang and Le Jinhua (South China University for Tropical Agriculture ,Haikou, Hainan 571101 China; Bioengineering College , Shihezi University ,Shihezi, Xinjiang 832003 China)  
Seed watermelon ( Citrullus lanatus ssp. vulgaris convar. megalaspermus Lin et Chao.) is the one convar. of ssp. vulgaris. It is one of the well-known, high quality seed-use watermelon. This paper reports how the factors affecting tissue culture as we aims to establish a quick, high frequency regeneration system of genetic translation for seed watermelon. The results indicated that the basal and middle segments of cotyledon of the young plants of 1 to 6 days age were the best appropriate explants. The combination of 6-BA 2 mg/L+IBA 0.1 mg/L was the optimum medium for differentiation of buds. The adventitious buds were found at the basal parts of central or lateral veins. The adventitious shoot elongation was the best on MS supplemented with 0.2 mg/L KT. On MS plus IAA 0.5 mg/L medium, the shoots stretched better and the rooting rates was 80%. This experiment has explored the suitable culture medium for bud inducing, shoot elongating and rooting which fit cotyledon. Through the test and verification at the National Key Biotechnology Laboratory for Tropical Crops, the results were proved to be steady and reliable, it is believed that the research will be useful for the seed watermelon tissue culture, utiliazation of so-.matic clonal variation and its genetic transformation.
【CateGory Index】: S651
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