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《Journal of Fruit Science》 2006-01
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Optimization of SRAP -PCR in some Diospyros spp.

GUO Da-long, LUO Zheng-rong*(Key Laboratory For Horticultural Plant Biology, MOE, Huazhong Agricultural University, Wuhan, Hubei 430070 China)  
SRAP is a new molecular marker which could provide high polymorphism and plentiful information. It is simple and has not the specie-specific character. It had been widely used for genetic diversity, comparing genome analysis and map construction, and so on. The concentrations of Mg2+, dNTPs, Taq DNA olymerase, primers which affect the SRAP-PCR reactions were optimized in order to establish the SRAP molecular marker system in Diospyros spp. The optimum system was as follows: template DNA 30 ng, Buffer 1×, Mg2+ 2.5 mmol/mL, dNTPs 0.2 mmol/L, Taq DNA polymerase 1 u, primer 0.3 μmol/L .The total volume of reaction was 25 μL. Amplications were carriyed out on 29 Diospyros spp. genotypes using this optimum system. The results showed that the system was steady and reliable and would be helpful to study origin and evolution of Diospyros spp.
【Fund】: 国家自然科学基金资助项目(30270925)。
【CateGory Index】: S665.2
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