Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Fruit Science》 2006-02
Add to Favorite Get Latest Update

Pollen viability of 43 pear cultivars and comparison of testing methods

JIANG Xue-ting, DU Yu-hu, ZHANG Shao-ling, WU Jun1(Research Institute of Pomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095 China; Liaoning Agricultural Vocation-Technical College, Yingkou, Liaoning 115009 China)  
Determination on pollen germination rate and the length of pollen tube of 43 pear cultivars were carried out by testing method of pollens cultured in vitro. Imamuraaki and Xuehuali , Gold-nijisseiki and Hosui , Qingsong and Mantianhong were chosen as experimental materials for determining their different pollen viability. Comparison of four testing methods, including I-KI coloration, blue-ink coloration, MTT coloration, peroxide enzyme coloration, were used. After 24h cultured, the average germination rate of 43 pear cultivars was 66.15%; among them 84% of the cultivars'germination rate was higher than 60% and the rate Mantianhong cultivar even reached 82.79%. The pollen tube length averaged 1.77 mm, which in 89% pear cultivars reached only 1 mm and the longest reached 3.25 mm. Results indicated that pollens in most cultivars were viable. Comparing the testing method of pollens cultured in vitro, the coloration results of living pollen showed that the statistic data from I-KI coloration was extremely lower, while that from MTT coloration was remarkably higher. So these methods are not suitable to be used for testing the pear pollen viability. The statistic data from peroxide enzyme coloration showed the pollen viability of Gold-Nijisseiki had high difference with that of control, so it is also not suitable to be used for testing the pollen viability of pears. Blue-ink coloration differed little from the germination rate testing method. So it is believed this method may be the better one for testing pear pollen viability.
【Fund】: 教育部科学技术重点资助项目(105089)。
【CateGory Index】: S661.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 ZUO Dan-dan et al (College of Horticulture and Landscape Architecture,Yangtze University,Jingzhou,Hubei 434025);Advance in Technique of Plant Pollen Viability[J];Journal of Anhui Agricultural Sciences;2007-16
2 LIU Da-sheng et al(Shandong Institute of Environmental Science,Jinan,Shandong 250013);Detection on Pollen Morphology and Viability of Alien Invasive Species Alternanthera philoxeroides[J];Journal of Anhui Agricultural Sciences;2008-35
3 REN Qiu-ping,ZHANG Bin-bin(Department of Horticulture Engineering,Agricultural College,Liaocheng University,Liaocheng,Shandong 252059,China);Effect of Different Preservation Temperature on Pollen Viability of Several Pear Cultivars[J];Northern Horticulture;2008-03
4 WANG Yong-Zhou,GU Song,JIANG Sha(Nankai University,Tianjin 300071,China);THE TESTING METHODS AND COMPARISON OF POLLEN VITALITY[J];Journal of Inner Mongolia Agricultural University(Natural Science Edition);2008-01
5 LI Xiao-fen,LIN Xin-chun,SHI Yan,GUO Xiao-qin,FANG Wei Key Lab for Modern Silvicultural Technology of Zhejiang Province,311300 Lin’an,Zhejiang,China;Flowering Biological Characteristics and Pollen Viability of Phyllostachys nigra[J];China Forestry Science and Technology;2009-06
6 SHEN Gen-hua QIAN Pei-hua JIN Feng-lei Songjiang District Pear Institute,Shanghai 201600,China; WANG Xiao-qing LUO Jun Forestry and Pomology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Lab of Protected Horticultural Technology,Shanghai 201106,China; ZHANG Shao-ling College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Effects of greenhouse culture on pear pollen quantity per anther and pollen viability[J];Acta Agriculturae Shanghai;2008-03
7 SUN Kun,ZHANG Yu-na,SU Xue,ZHANG Hui,LI Jin-xia,WEN Jiang-bo(College of Life Science,Northwest Normal University,Lanzhou 730070,Gansu,China);Comparison of the detection methods of pollen viability of Hippophae rhamnoides L.ssp.sinensis Rousi[J];Journal of Northwest Normal University(Natural Science);2009-02
8 YUAN De-yi,TAN Xiao-feng,HU Qing-su,ZOU Feng(The Key Lab.Of Non-wood Forest Product of Forestry Ministry,Central South University of Forestry and Technology,Changsha 410004,China);Study on Camellia Pollen Characteristics and the Vitality under Different Storage Conditions[J];Journal of Zhejiang Forestry Science and Technology;2008-05
9 HE Chun-yan,TAN Xiao-feng,YUAN De-yi,HU Qing-su,ZOU Feng (The Key Lab.of Non-wood Forest Products of Forestry Ministry,Central South University of Forestry and Technology,Changsha 410004,Hunan,China);Determination of the Pollen Number and Pollen Germination Rate of Seven Camellia Species[J];Journal of Central South University of Forestry & Technology;2009-01
10 Li Gang1,Gao Min1,Wang Zibin2,Cheng Shiping1,Shi Jiang1(1College of Agronomy,Luoyang Key Laboratory of Peony Biology,Henan University of Science & Technology,Luoyang 471003 ;2Editorial Department of Journal of Yangzhou University,Jiangsu Yangzhou 225009);Study on the Detection Methods of Long-term Storage Pollen Viability of Paeonia lactiflora Pall[J];Chinese Agricultural Science Bulletin;2008-12
【Citations】
Chinese Journal Full-text Database 6 Hits
1 XU Guo-hua~(1,2), WU Hua-qing~1, ZHANG Shao-ling~(1,*) (1 College of Horticulture, Nanjing Agricultural University, Nanjing 210095,China; 2 Department of Biology, Jiangsu College of Education, Nanjing 210013,China);Study on identification of S-genotypes of Chinese pear cultivars[J];Acta Botanica Boreali-occidentalia Sinica;2004-10
2 YU Xiao-yinga,b,LU Xiang-yangb,GONG Ming-fua,XIE Zhi-juna,CAO Huia,DING Jiang-nana (a.College of Horticulture and Landscape;b.Lab of Biochemistry and Fermentation Engineering,HNAU,Changsha 410128,China);Pollen Viability of Senecio hybridus[J];Journal of Hunan Agricultural University;2005-01
3 HE Xiao-di~1,ZHAO Zheng-lan~2,ZHOU Kui~3,WU Fa-ming~4,XU Wei-wei~1,LIU Hua~5(1.School of Agricuture Yangzhou University,Yangzhou 225009,China;2.Huai’an City Landscape Bureau,Huai’an 223001,China;3.Gaochun City Construction Bureau,Gaochun 211300,China;4.Wujin ChemicalFertilizer Plant,Wujin 213100,China;5.Wuxi City Agriculture and Forest Bureau,Wuxi 214023,China);The Comparison of Pollen Viability Determination among 17 Ornamental Trees of Prunus[J];Journal of Nanjing Forestry University;2005-02
4 Zhang Shading Xie Wennuan Chen Dixin Gao Fuyong (College of Horticulture,Nanjing Agricultural University, Nanjing 210095);THE DIFFERENCES OF POLLEN CONTENT AND POLLEN GERMINATION AND TUBE GROWTH AMONG EIGHT SPECIES OF FRUIT TREES[J];Acta Agriculturae Shanghai;2003-03
5 He Tianming, Zhang Qi, Cheng Qi\;(Institute of Plant Science,Tarim Agricultural Reclamation University,Alar,843000);Study on the Floral Characteristics and Pollen Activity of Xiangli (Pyrus bretschneideri Rehd)[J];Journal of Xinjiang Agricultural University;2000-03
6 Zhang Zixue Sun Feng (Anhui Technical Teachers College 233100);The Selection of the Best Testing Method on the Pollen Viability in Hot Pepper[J];Seed;2002-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Yu-xia1,2,WANG Ying1,LI Fang-dong1,2,WEI Xin1,2,DONG Wen-xuan1,2*,(1.College of Horticulture,Shenyang Agricultural University,Shenyang 110161,China;2.Research Institute for Breeding and Physiological Ecology of Frigid Region Fruit Tree,Shenyang 110161,China);Study of Pollen Quantity and Vitality of Hawthorn Cultivars with Different Kernel Percentage[J];Shandong Agricultural Sciences;2008-02
2 WANG Fei et al (College of Horticulture,Shenyang Agricultural Universtiy,Shenyang,Liaoning 110161);Study on the Characteristics of Pollen Germination of Raspberry[J];Journal of Anhui Agricultural Sciences;2006-19
3 ZUO Dan-dan et al (College of Horticulture and Landscape Architecture,Yangtze University,Jingzhou,Hubei 434025);Advance in Technique of Plant Pollen Viability[J];Journal of Anhui Agricultural Sciences;2007-16
4 WANG Ding-kang et al (Department of Biology,Kunming College,Kunming,Yunnan 650031);Comparison of Determination Methods of Paris polyphylla var.yunnanensis Pollen Activity[J];Journal of Anhui Agricultural Sciences;2007-35
5 WANG Xiao-peng et al (College of Forestry,Shenyang Agricultural University,Shenyang,Liaoning 110161);Screening of the Optimum Method for Determining the Pollen Viability of Viola tricolor Linn[J];Journal of Anhui Agricultural Sciences;2008-07
6 WANG Ding-kang et al(Department of Biology,Kunming College,Kunming,Yunnan 650031);Comparison of Determination Methods of Cynanchum otophyllum Schneid Pollen Activity[J];Journal of Anhui Agricultural Sciences;2008-12
7 LIU Da-sheng et al(Shandong Institute of Environmental Science,Jinan,Shandong 250013);Detection on Pollen Morphology and Viability of Alien Invasive Species Alternanthera philoxeroides[J];Journal of Anhui Agricultural Sciences;2008-35
8 SUN Yi1,LI Bao-jiang1,ZHANG Mao-jun2 (1 Horticulture College of Shenyang Agriculture University,Shenyang 110161,China; 2 Pomology Institute of Jilin Agriculture Academy,Gongzhuling,Jilin 136100,China);Studies about the Characteristics of Flowers and Pollen of Pyrus ussuriensis[J];Northern Fruits;2008-03
9 LI Xiao-li,YAN Li-ying,FENG Zhi-hong,JIA Yin-xia(Dept.of Horticulture and Landscape,HNUST,Qinhuangdao,Hebei 066600,China);Studies on the Pollen Viability of Cucumber[J];Northern Horticulture;2008-03
10 WANG Fu1,LI Wen-li1,QIN Gong-wei2,YUAN Zhen-xin1 (1.College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China;2.Biological Science and Engineering Department,Shanxi University of Technology,Hanzhong,Shanxi 723000,China);Screening of the Optimal Liquid Culture Medium for Tomato Pollen Germination[J];Northern Horticulture;2008-11
China Proceedings of conference Full-text Database 3 Hits
1 Liu Debing ,He Ruiai ,Fan Chonghui,Wei Junya and Li Shaopeng ( Horticulture College,South China University of Tropical Agriculture, Danzhou Hainan 571737 ; Northwest Sci-Tech University of Agriculture & Forestry,Yangling Shaanxi 712100j3 Life Science College, Zhongshan University, Guangzhou 510275,China);The Study on Comparison of Determine Chinese Wampee Pollen Activity[A];[C];2004
2 FU Xi-ling GUO Xian-feng~① DING Xiu-tang DU Ming-yun (College of Forestry,Shandong Agricultural University,Tai'an 271018);A Study on Characteristics of Pollen Germination and Storage of 3 Wild Herbaceous Peonies[A];[C];2007
3 ;A Study on the Pollen Viability and Storage Time of Pingyin Rosa[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Fei et al (College of Horticulture,Shenyang Agricultural Universtiy,Shenyang,Liaoning 110161);Study on the Characteristics of Pollen Germination of Raspberry[J];Journal of Anhui Agricultural Sciences;2006-19
2 WANG Wen-yuan et al(Shenyang Agricultural University,Shenyang,Liaoning 110161);Effect of Different Temperature and Preserved Condition on the Pollen Viability of Iris[J];Journal of Anhui Agricultural Sciences;2006-23
3 ZUO Dan-dan et al (College of Horticulture and Landscape Architecture,Yangtze University,Jingzhou,Hubei 434025);Advance in Technique of Plant Pollen Viability[J];Journal of Anhui Agricultural Sciences;2007-16
4 LIU Tong-kai et al(Henan Yellow River Landscape Limited Company of Greening Engineering,Zhengzhou,Henan 450003);Study on Grafting Technique of Acer rubrum[J];Journal of Anhui Agricultural Sciences;2009-05
5 YANG Ming-sheng, LI Tian-hui (China Eucalypt Research Center, Zhanjiang 524022, Guangdong, China);Progress in the research of Eucalyptus in China and Its Scientific Management[J];Eucalypt Science&Technology;2005-02
6 CAO Jia-guang (China Eucalypt Research Center,Zhanjiang Guangdong 524022);Studied on Once Only Controlled Pollination of Eucalyptus[J];Eucalypt Science & Technology;2006-02
7 LI Ping1,ZHANG Chao-hong2,AI Shao-zhou1,MA Sheng-ping1(1 Suide Supervision and Control Bureau of Water and Soil Conservation of the Yellow River Suide,Shannxi 718000,China;2 Horticulture College of Northwest Agriculture and Forestry University of Science and Technology,Yangling,Shannxi 712100,China);Experiment about influence factors of kiwi pollen germination[J];Northern Fruits;2007-01
8 XING Xin-ting~1;FU Mao-yi~1;XIAO Xian-tan~2.1 Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang City,Zhejiang Province,311400,P.R.China;2 Forest Bureau,Nanjing County,Fujian Province,363600,P.R.China.;Biological characteristics of flowering and controlled pollination of Dendrocalamus latiflorus Munro[J];Journal of Beijing Forestry University;2005-06
9 ZHANG Ya-li; SHANG Xiao-qian; LIU Yan. College of Landscape Architecture, National Floriculture Engineering Research Center, Beijing Forestry University, 100083, P. R. China.;Advances in research of pollen cryopreservation[J];Journal of Beijing Forestry University;2006-04
10 Fu Yulan (Anhui Agricultural University, Hefei,230036,P.R.China);Research on Pollen Shape of New Cutivars of Dendranthema × grandiflorum cv.Hanju[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1998-02
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 ZHANG Hong-jian et al (Weinan Vocational Technology College,Pucheng,Shaanxi 715501);Preliminary Study on Pollination and Fruiting Ability of Zaosu Pear[J];Journal of Anhui Agricultural Sciences;2008-26
2 JIA Ji-wen1,2,WANG Jun-hui1,ZHANG Jin-feng2,ZHANG Shou-gong1,ZHANG Jian-guo1,ZHAO Kun3(1 Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091,China;2 Beijing Forestry University,National Engineering Laboratory for Tree Breeding,Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing 100083,China;3 Luoyang Forestry Research Institute,Luoyang,He'nan 471002,China);Pollen Vitality of Catalpa Scop.[J];Acta Botanica Boreali-Occidentalia Sinica;2009-05
3 LEI Bao-sheng,ZHOU Lan-ying,DONG Hui,YUAN Ding-chang,XIANG Qian Forestry and Hoticulture College,Sichuan Agiriculture University,625014 Ya'an ,Sichuan,China;Effects of Different Preservation Temperature and Time on Jatropha curcas Pollen Viability[J];China Forestry Science and Technology;2009-02
4 ZOU Feng,TAN Xiao-feng*,YUAN De-yi,XIE Peng,YUAN Jun (The Key Lab of Non-wood Forest Products of Forestry Ministry,Central South University of Forestry and Technology,Changsha 410004,China);A Study on Camellia Pollen Number and the Vitality Change under 4 ℃ Storage[J];Acta Agriculturae Universitatis Jiangxiensis;2009-05
5 SUN Kun,ZHANG Yu-na,SU Xue,ZHANG Hui,LI Jin-xia,WEN Jiang-bo(College of Life Science,Northwest Normal University,Lanzhou 730070,Gansu,China);Comparison of the detection methods of pollen viability of Hippophae rhamnoides L.ssp.sinensis Rousi[J];Journal of Northwest Normal University(Natural Science);2009-02
6 YUAN De-yi,TAN Xiao-feng,ZOU Feng,CUI Ming-jie,LIANG Yu-qin(Key Lab of Non-timber Forest Tree Species Breeding and Cultivation,SFA,South Central University of Forestry Science and Technology,Changsha Hunan 410004,China);Comparative Study on Pollen Viability Detection Methods for Camellia oleifera[J];Journal of Southwest Forestry University;2009-04
China Proceedings of conference Full-text Database 3 Hits
1 ZUO Dan-dan~(1,2) MING Jun~1 LIU Chun~1 MU Ding~1 WANG Li-na~(1,2) (~1 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081; 2 Yangtze University,School of Gardening and Horticultur'e,Jingzhou 434023);Pollen Viability Test of Chimonanthus praecox[A];[C];2007
2 WANG Lei WANG Jin-gang FAN Jin-ping GONG Shu-fang CHE Dai-di (College of Horticulture,Northeast Agricultural University,Ha'erbin 150030);Cross Breeding and in vitro Seedling Techniques of Interspecific Hybridization on Gladiolus hybridus Hort.[A];[C];2008
3 Huang Guihua,Liang Kunnan,Zhou Zaizhi,Ma Huaming,Lin Mingping (Research Institute of Tropical Forestry,CAF,Guangzhou 510520,Guangdong,China);In Vitro Pollen Germination and Pollen Tube Growth of Teak (Tectona grandis L.f.)[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Siqing Chen Junyu(College of Landscape Achitecture, BFU);Pollen Germination of Dendranthema×grandi flora and Some Other Plants of Compositea in Vitro[J];Journal of Beijing Forestry University;1993-04
2 Xu Jizhong; Chen Haijiang; Shao Jianzhu; Wang Yanhui (Department of Horticulture, Hebei Agricultural University, Baoding 071001; School of Agronomy, Hebei Agricultural University, Baoding 071001);Effects of Exogenous Polyamines and Their Inhibitor MGBG on the Apple Pollen Germination and Fruit Set[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-04
3 CHEN Gui xin 1, PAN Dong ming 1, LU Liu xin 1, WANG Feng hua 2, LAI Zhong xiong 2 (1.College of Horticulture;2.Institute of Subtropical Fruits, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Advances in the techniques for extraction of nuclear DNA and isolation and cloning of target genes in fruit trees[J];Journal of Fujian Agricultural University(Natural Science);2002-01
4 Guan Junfeng;Ma Zhihong;Zhang Xiaomin;Li Minxia; and Zhang Xinzhong(Department of Horticulture, Hehei Agrotechnical Teachers College,Changli 066600; Changli Institute of Pomology,Hebei Academy of Agricultural Science);Ca~(2+) in Relation to Germination and Tube Growth of Apple Pollen[J];;1999-03
5 Zhang Shaoling,Cao Shengmin,and Wu Huaqing (College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095China);Self-incompatibility Genotypes of Fruit Trees and Their Identification Me thods[J];Journal of Fruit Science;2003-05
6 Zhou Huaijun, An Lianrong, Zhu Zhefeng, Xu Jing, Wang Delai (Forestry College, Agriucultrural University of Hebei, Baoding 071000);EXPERIMENT ON POLLEN GERMINATION OF DIFFERENT APRICOT VARIETIES AND FRUIT BRANCH TYPES[J];HEBEI JOURNAL OF FORESTRY AND ORCHARD RESEARCH;2000-03
7 ZHAO Changxing,LIU Chenglian (Horticultural Department of Laiyang Agricultural College, Laiyang,Shandong, 265200);ELEMNTARY STUDIES ON MEASURATION OF LIFE-ABILITY OF FRUIT TREE POLLEN[J];Hebei Journal of Forestry and Orchard Research;2001-03
8 WANG Yan,ZHANG Li ming (Department of Horticulture, Hubei Agricultural College,Jingzhou,Hubei 434025,China);Influences of Storage Temperatures on Activated Pollen Ratio in Ginkgo biloba[J];Journal of Hubei Agricultural College;2002-03
9 Huang Junyuan1 Zhen Anxin1 Zheng Suoqiu2(1 Lengshuijiang Vegetables Research Institute of Hunan,Lengshuijiang,417500)(2 College of Plant Science & Techonology,HNAU,Changsha,410128);STUDIES ON OPTIMIZATION OF POLLEN GERMINATION MEDIA FOR SEVERAL SUMMER VEGETABLES[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;1997-06
10 Wang Shaoxian (Luoyang Agricultural College,Luoyang 471003);Effect of Four Testing Methods to Detect the Pollen Viability of Hot Pepper[J];JOURNAL OF HENAN AGRICULTURAL SCIENCES;1998-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved