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《Journal of Fruit Science》 2006-04
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Analysis of genetic relationship of 15 Vitis germplasm resources by ISSR markers

WU Zi-long1, FANG Lian-yu1, WANG Jun1*, SHEN Yu-jie2 ( 1College of Forestry, Northeast University of Forestry, Harbin, Heilongjiang 150040 China; 2Research Institute of Special Wild Economic Animals and Plants, CAAS, Jilin 132109 China)  
15 Vitis germplasm were used as materials for analyzing their genome polymorphism by ISSR markers. 15 primers selected from 60 primers were used for ISSR amplification. A total of 105 bands were generated, of which 91 bands were polymorphic bands (the percentage of polymorphic band, PPB=86.7%). According to the results of ISSR amplification, which were analyzed to Jaccard similarity coefficient by NTSYSpc2.10e software, the genetic similarity coefficient was 0.27 to 0.75. The average genetic similarity coefficient was 0.471. The clustering dendrogram was constructed by UPGMA method. 15 Vitis materials were divided into two major groups while the similarity coefficient was 0.39. The fist group included 7 V. amurensis materials, 2 cultivars of hybrids (V. amurensis×V. vinifera) ×V. amurensis. The second group included 3 cultivars of V. vinifera, 2 cultivars of Euro-American hybrids and 2 cultivar of American grape hybrids. The results showed that the relationships between V. amuensis and V. vinifera, Euro-American hybrid, American hybrids had a far similarity, but the relationships between V. vinifera and Euro-American hybrid, American hybrids had a closer similarity.
【Fund】: 国家林业局动植物保护基金(010-413255);; 黑龙江省博士后基金;; 东北林业大学科研基金资助项目
【CateGory Index】: S663.1
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