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《Journal of Fruit Science》 2008-03
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Assessment of pear germplasm from the middle area of Gansu province using amplified fragment length polymorphism markers

CAI Dan-ying1,FAN Tai-wei2,TENG Yuan-wen1*,ZHAO Chang-zeng2,CHEN Bai-hong2,WANG Fa-lin3 (1Department of Horticulture, Zhejiang University, the State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development & Biotechnology, Hangzhou, Zhejiang 310029 China; 2Department of Horticulture, College of Agronomy, Gansu Agricultual University, Lanzhou, Gansu 730070 China; 3Fruit Research Institute, Gansu Academy of Agriculturial Sciences, Lanzhou, Gansu 730070 China )  
Genetic diversity and genetic relationship of 40 accessions of pear (Pyrus L.) germplasm native to the middle area of Gansu province in China were analyzed using amplified fragment length polymorphism (AFLP)marker. 6 primer combinations generated 472 scored bands, among which 404 (86%) were polymorphic, which showed the abundant genetic diversity of these pear gerrmplasm. Based on the UPGMA cluster analysis, 40 pear accessions could be obviously divided into 7 major groups at the level of the coefficient of 0.72: P. communis L, P. sinkiangensis Yu, P. pyrifolia Nakai var. sinensis Y. Teng et K. Tanabe (Chinese white pears), P. xerophila Yu, P. phaeocarpa Rehd. and two P. ussuriensis Maxim groups. All the Chinese white pears were clustered into one group with Huanghuali, which belonged to P. pyrifolia. Unidentified pear cultivars or types were clustered with P. ussuriensis, P. communis, P. xerophila and Chinese white pears, respectively. The research indicated that the classification system based on AFLP could reflect the genetic diversity and relationship of Pyrus species and cultivars from Gansu province.
【Fund】: 国家自然科学基金(3037098)
【CateGory Index】: S661.2
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