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《Journal of Fruit Science》 2008-03
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Advances in application research on genetic transformation of fruit trees via particle bombardment

TONG Zhao-guo1, CAI Bin-hua1, ZHANG Zhen1*, WANG Fu-rong2, ZHANG Kai-chun3, YAN Guo-hua3, ZHOU Yu3 (1College of Horticulture,Nanjing Agricultural University, Nanjing, Jiangsu 210095 China; 2Institute of Pomology and Tea, Hubei Academy of Agricultural Science,Wuhan, Hubei 430209 China; 3Institute of Forestry and Pomology,Beijing Academy of Agricultural and Forestry Sciences, Beijing 100093 China)  
Nowadays the main methods of gene transformation and their applications in fruit trees are Agrobacterium-mediated transformation and direct DNA uptake. This review introduces the basic principle, types and important factors, such as bombardment parameters, explant, genotype, culture conditions of pre-and post-bombardment, that influence transfer efficiency about particle bombardment which is one of the methods of direct DNA uptake and systematically summarizes its application in genetic transformation of selectable marker gene, report gene, interest genes of genetic improvement of fruit tree traits, promoter and multiple genes. In addition, the prospects of its application and several main programs are put forward.
【Fund】: 国家自然科学基金(30370987 30671438)
【CateGory Index】: S66
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