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《Journal of Fruit Science》 2009-05
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Studies on fingerprinting of seedless triploid watermelon cultivars and hybrid purity identification by SRAP markers

ZHAO Sheng-jie,LIU Wen-ge,YAN Zhi-hong,HE Nan(Zhengzhou Fruit Research Institute,CAAS,Zhengzhou,Henan 450009 China)  
SRAP(Sequence-Related Amplified Polymorphism) marker was applied to analysis DNA fingerprinting of 9 triploid seedless watermelon cultivars and identify hybrid purity.The results showed that 24 primer pairs selected from 30 amplified 109 polymorphic bands with an average of 4.5 polymorphic bands per primer pairs and the polymorphic rate of primer pairs was 12.5% to 80%.All 9 cultivars could be distinguished from each other combining several SRAP primer pairs.In the fingerprinting analysis of Heimi No.5 and its parents,specific bands were generated on Heimi No.5 with ME4/EM3 primer pairs and enabled to distinguish the female parent from the F1 hybrid.This primer pair was certified to be suitable for purity identification of Heimi No.5 in experiments.
【Fund】: 国家科技支撑计划(2006BAD01A7-6-03);; 河南省重大科技攻关(072101110500);; 河南省自然科学基金项目(082300430170)
【CateGory Index】: S651
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