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《Journal of Fruit Science》 2012-03
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Genetic relationship of Chinese jujube(Ziziphus jujuba) and sour jujube (Z.spinosa) based on RAPD markers

LI Rui-huan,LI Xin-gang*,HUANG Jian,GAO Wen-hai (College of Forestry,Key Laboratory of Siviculture on the Loess Plateau of State Forestry Administration,Northwest A & F University,Yangling,Shaanxi 712100 China)  
The genetic relationship of 25 Chinese jujube(Ziziphus jujuba Mill.) and 19 sour jujube(Z.spinosa Hu.) sampled primarily from the origin center of Chinese jujube,the Yellow River Canyon located in North Shaanxi,were analyzed using RAPD.Thirteen primers selected from 50 10-mer arbitrary ones were employed to make RAPD analysis for 44 materials.High polymorphic were identified,and a total of 195 DNA bands were amplified,among which 161(82.56%) were polymorphic bands,15 bands were obtained per primer in average.The results revealed abundant genetic diversity in the 44 germplasms on DNA level.Tree diagrams were established by UPGMA methods,and the 44 germplasms could be divided into three clusters based on genetic distance of 22.0.It was not that all of the sour jujube or jujube come to one cluster,but the ones from the near places came into a cluster.It was displayed a genetic way as sour jujube→transition-sour jujube→Chinese jujube.The evolution from sour jujube to Chinese jujube was multi-way.As the origin place of Chinese jujube,the Yellow River Canyon had more complicated genetic background in the evolution of Chinese jujube and sour jujube.In this area,sour jujube,transition-acid jujube and Chinese jujube came out closer relationship,presented the character of the origin region.It was further revealed that the genetic relationship in the origin was more representative.
【Fund】: 唐仲英育种基金;; 国家林业局新品种保护项目(2004010)
【CateGory Index】: S665.1
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