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Method for extraction, isolation and bioassay of antifungal compounds from Pyrus Pyrifolia cv Pingguoli

Li Yongcai(Department of Horticulture, Gansu Agricultural University, Lanzh0u 730070)Bi Yang; Cao Jiankang(Department of Food Engineer, Gansu Agricultural University)  
Active antifungal compounds cou1d be obtained by re-extraction ethanol crudeextract with ethylacetate or dichloromethane and concentfation under vaccum from the peel ofunripe Pingguoli pear. The compounds in the extract separated by thin-layer chromatographicplates (TLC) and detected the antifungal compounds in the isolates on the TLC plate with conidiaof Alternaria alternata. The results showed that separating compounds using n-hexane: ethylacetate: methanol at a 60: 40: 15 ratio was better and Rf of its inhibition area was about 0.4~0.6.
【Fund】: 甘肃省农委资助!9870599
【CateGory Index】: S661.2
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