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《Journal of Gansu Agricultural University》 2007-03
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Preparation of colloidal gold labelling strips with the 6H4 strain of monoclonal antibody against the type O foot and mouth disease

LIN Tong1,2,CHANG Hui-yun1,WU Hai-bo1,SHAO Jun-jun1,DU Jun-zheng1,CONG Guo-zheng1,LI Ke-sheng3,DU Hui-fen3(1.Key Laboratory of Animal Virology,Ministry of Agriculture;State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China; 3.Center of Medical Biotechnology,Gansu Academy of Medical Sciences,Lanzhou 730050,China)  
Based on prepared monoclonal antibody(McAb) against the type O foot and mouth disease virus(FMDV),the 6H4 strain of McAb was applied to develop a new kind of McAb colloidal gold labelling strips by immune colloidal gold technique,aiming at obtaining a extraodinary,handy,rapid and audiovisual way of foot and mouth disease(FMD) detection and diagnosis.The colloidal gold particles were synthesized were prepared by sodium citrate reduction method and used as the labelling subjects of the 6H4 McAb.The results showed that the 6H4 strain of McAb was able to prepare the colloidal gold labelling antibody and its reactive membrane,and the concenration stable point of its McAb purified protein in the pH8.64 colloidal gold solution was calculated to be 12 mg/L.
【Fund】: 国家“863”高技术研究发展计划(2003AA241110).
【CateGory Index】: S854.43
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Chinese Journal Full-text Database 2 Hits
1 ZHANG Gai-ping,LI Xue-wu,YANG Yan-yan,DENG Rui-guang,LI Qing-mei(Institute of Biology and Technique Science,Henan Agricultural Sciences,Zhengzhong 450002,China);Development and Trait Identification of the Rapid Diagnosis Strips of the Infectious Bursal Disease[J];Chinese Journal of Animal and Veterinary Sciences;2004-01
2 WANG Zhong-li~(1), SONG Xiao-hui~(2), CHEN Xi-zhao~(3), CHEN Guo-sheng~(1) 1. National Animal Husbandry & Veterinary Service,Beijing 100026,China; 2.China Agricultural University College of Veterinary Medicine, Beijing 100094 China; 3.Minisry of Agricultural National Veterinary Diagnostic Center ,Beijing 100094 China);The research of gold-immunochromatography for CPV diagnostic[J];Chinese Journal of Preventive Veterinary Medicine;2004-01
Chinese Journal Full-text Database 10 Hits
1 SUN Su-ling(Anhui Science and Technology University,Fengyang,Anhui 233100);Development of Colloidal Gold Rapid Diagnostic Stripe against Chicken Infectious Anaemia[J];Journal of Anhui Agricultural Sciences;2008-12
2 LIU Zhi-jun et al(Animal College of Science and Technology,Henan University of Science and Technology,Luoyang,Henan 471003);The Pathology Observation of the Dogs Infected Naturally by Canine Parvovirus[J];Journal of Anhui Agricultural Sciences;2008-30
3 YAN Wen-qing,WU De-feng,DAI Ya-dong,LIN Yong-li(College of Animal Science,Fujian Agricultural and Forestry University,Fuzhou,Fujian,350001,China);Study on the Pathogeny of Canine parvovirus[J];Progress in Veterinary Medicine;2006-01
4 SHI Li-jun,ZENG Ni,LI Gang(Institute of Animal Science and Veterinary Medicine,Chinese Academy of Agricultural Science,Beijing,100193,China);Progress on the Biological Characteristies and Detection Techniques of CPV[J];Progress in Veterinary Medicine;2010-04
5 ZU Li-chuang1,WANG Jin-liang2,LI Jiao1,SHEN Zhi-qiang1,2 (1.Shandong Lvdu Bio-Sciences & Technology Co.Ltd.,Binzhou,Shandong,256600,China;2.Shandong Binzhou Animal Science&Veterinary Medicine Academy,Binzhou,Shandong,256600,China);Application of Gold Immunochromatography Assay in Animal Disease[J];Progress in Veterinary Medicine;2010-08
6 WANG Jian-ke,YI Li,LUO Bin,YANG Shen,CHENG Shi-peng(Institute of Special Wild Economic Animal and Plant Science,CAAS.Jilin,Jilin,132109,China);Application of Gold Immunochromatographic Technique in Detection of Animal Viral Infectious Diseases[J];Progress in Veterinary Medicine;2011-01
7 WANG Wei-fang1 LI Qing-mei2 GUO Jun-qing2 LEI Ji-lin1(1Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,266071)(2Henan Key Laboratory for Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002);Progress and prospect of colloidal gold-based immunochromatographic lateral flow assay and its application in aquaculture[J];Progress in Fishery Sciences;2010-03
8 LI Yin~(1,2),LIU Yu-zhuo~(2), ZHANG Jing-feng~(2),SHEN Yong-lin~(1),ZHU Yuan-li~3(1.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;3.Bureau of Finance and Capital Construction,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Preparation and Biological Characterization of Monoclonal Antibodies against H9 Subtype of Avian Influenza Virus[J];Jiangsu Journal of Agricultural Sciences;2006-02
9 JIANG Hui(Maternal and Child Health Hospital of Xiangyang City,Hubei 441003,China);The comparison of hemafecia test methods[J];Laboratory Medicine and Clinic;2011-21
10 SUN Xiao-dong DENG Yan ZHANG Zai-xing (Yunnan Provincial Institute of Parasitic Diseases,Simao 665000,Yunnan,P.R.China);Evaluation of results of ACON Malaria p.f./p.v.Gold Immunochromotographic Kit in diagnosis of Plasmodium falciparum and Piasmodium vivax[J];China Tropical Medicine;2007-01
China Proceedings of conference Full-text Database 1 Hits
1 ZHAO Yu-long~1,JIAO YuLan~1,YU HongWei~1,Ma-Ming (1.Rinpu(Baoding)Biological Pharmaceutical Co.,Ltd,Baoding 071000);Development Study of Colloidal Gold Immunochromatographic Rapid Diagnostic Test Strip[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 DONG Jiang li,LI Wu,E Ri he,ZHANG He ling (Department of Biology,NeiMongol University,Hohhot 010021,PRC);Detection of Canine Parvovirus by Polymerase Chain Reaction[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NEIMONGOL;2000-03
2 HUANG Guang ming,ZHANG Man fu  (College of Biological Science, China Agricultural University,Beijing 100094,China);Detection of Infectious Bursal Disease Viruses by in situ PCR[J];CHINESE JOURNAL OF VETERINARY SCIENCE;2000-06
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