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《Journal of Gansu Agricultural University》 2007-03
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Preparation of colloidal gold labelling strips with the 6H4 strain of monoclonal antibody against the type O foot and mouth disease

LIN Tong1,2,CHANG Hui-yun1,WU Hai-bo1,SHAO Jun-jun1,DU Jun-zheng1,CONG Guo-zheng1,LI Ke-sheng3,DU Hui-fen3(1.Key Laboratory of Animal Virology,Ministry of Agriculture;State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China; 3.Center of Medical Biotechnology,Gansu Academy of Medical Sciences,Lanzhou 730050,China)  
Based on prepared monoclonal antibody(McAb) against the type O foot and mouth disease virus(FMDV),the 6H4 strain of McAb was applied to develop a new kind of McAb colloidal gold labelling strips by immune colloidal gold technique,aiming at obtaining a extraodinary,handy,rapid and audiovisual way of foot and mouth disease(FMD) detection and diagnosis.The colloidal gold particles were synthesized were prepared by sodium citrate reduction method and used as the labelling subjects of the 6H4 McAb.The results showed that the 6H4 strain of McAb was able to prepare the colloidal gold labelling antibody and its reactive membrane,and the concenration stable point of its McAb purified protein in the pH8.64 colloidal gold solution was calculated to be 12 mg/L.
【Fund】: 国家“863”高技术研究发展计划(2003AA241110).
【CateGory Index】: S854.43
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【Citations】
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1 SUN Su-ling(Anhui Science and Technology University,Fengyang,Anhui 233100);Development of Colloidal Gold Rapid Diagnostic Stripe against Chicken Infectious Anaemia[J];Journal of Anhui Agricultural Sciences;2008-12
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