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《Journal of Gansu Agricultural University》 2008-03
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Molecular cloning and structure analysis of non-coding region of porcine reproductive and respiratory syndrome virus GS strain

MA You-ji1,2,LIU Ji-xing2,ZHANG Li2 (1.College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;2.Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)  
According to the sequence of porcine reproductive and respiratory syndrome virus(PRRSV)VR-2332 published in GeneBank,the primers were designed to amplify the sequence of non-coding region(NCR).The results showed that the length of 5′NCR is 190 bp,and the homology at the nucleotide levels between GS and VR-2332,LV was found to be 93.2 % and 61.1 % respectively,initiation codon was followed ORF1 with a conservative sequence:5′-UUUAACC-3′,its rolewas to connect the 5′ leader sequence with the group of subunit mRNAs.The length of 3′NCR is 151 bp,followed by a long 14 base pairs of poly(A) tail,the area of nuclear glycosides acid sequence was more conservative,and the homology between GS and VR-2332,LV was 95.4 % and 78.2 %,the upstream of poly(A) tail hasa highly conservative eight nucleic acid sequence:5′-CCGAAATT-3′,play an important role in the virus replication process.The secondary structure of non-coding region is complex.
【Fund】: 国家社会公益基金研究专项(2005DIB4J041);; 全国博士后基金(56341)
【CateGory Index】: S852.65
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