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《Journal of Gansu Agricultural University》 2010-05
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IGS-RFLP of small-spored Alternaria

YUE Hai-mei1,ZHUANG Hua2,WANG Mu1(1.Plant Science and Techology Department,Tibet Agricultural and Animal Husbandry College,Linzhi 860000,China;2.College of Plant Protection,Northwest Agriculture and Forestry University,Yangling 712100,China)  
Intergenic spacer was amplified with specific primers to analysis the small-spored species and big-spored(Alternaria solani)species.The small-spored ones could produce a single band about 3.0kb,while A.solani could produce a 4.0kb band,so the small-spored species and big-spored species could be distinguished easily by products of IGS.The PCR products were digested with restriction endoenzyme HaeⅢand MspⅠrespectively,and different patterns were obtained from each enzyme,by combining the restriction patterns of two enzymes,Alternaria alternataand Alternaria tenuissimacould be differentiated from each other.IGS displayed polymorphism in the length and the sequence structure,it could be treated as a good genetic marker and significant to identify kinds of different strains.For some isolates could not get PCR products,so its applying value is limited.
【Fund】: 国家自然科学基金项目(30760005)
【CateGory Index】: S432.44
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