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《Journal of Gansu Agricultural University》 2010-05
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IGS-RFLP of small-spored Alternaria

YUE Hai-mei1,ZHUANG Hua2,WANG Mu1(1.Plant Science and Techology Department,Tibet Agricultural and Animal Husbandry College,Linzhi 860000,China;2.College of Plant Protection,Northwest Agriculture and Forestry University,Yangling 712100,China)  
Intergenic spacer was amplified with specific primers to analysis the small-spored species and big-spored(Alternaria solani)species.The small-spored ones could produce a single band about 3.0kb,while A.solani could produce a 4.0kb band,so the small-spored species and big-spored species could be distinguished easily by products of IGS.The PCR products were digested with restriction endoenzyme HaeⅢand MspⅠrespectively,and different patterns were obtained from each enzyme,by combining the restriction patterns of two enzymes,Alternaria alternataand Alternaria tenuissimacould be differentiated from each other.IGS displayed polymorphism in the length and the sequence structure,it could be treated as a good genetic marker and significant to identify kinds of different strains.For some isolates could not get PCR products,so its applying value is limited.
【Fund】: 国家自然科学基金项目(30760005)
【CateGory Index】: S432.44
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2 JIANG Shu-jun;THE APPLICATION OF RAPD ON THE STUDY OF FUNGLE PLANT DISEASE[J];Journal of Heilongjinag August First Land Reclamation University;2001-01
3 WEI Song hong 1,2 , WANG Gang 1, ZHANG Ling bing 1, ZHANG Yan zhen 1, YANG Jia shu 2 (1.Research Center for Plant Genetic Engineering,The Quartermaster University of PLA, Jilin Changchun 130062,China; 2 Department of Plant Protection,;Determination and RAPD Analysis of Physiological Strain of Erysiphe graminis in Northeasten Spring Wheat Area of China[J];Journal of Jilin Agricultural University;2001-02
4 Zhu Heng Qu Feng Zhu Lihuang (Institute of Genetics, Academta Sinica, Beijing 100101);ISOLATION OF GENOMIC DNAS FROM FUNGI USING BENZYL CHLORIDE[J];Mycosystema;1994-01
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7 Li Zeng-Zhi; Huang Bo ;Fan Met-Zhen(College of Forest Utilizatin, Anhui Agricultural University, Hefei, Anhui 230036);MOLECULAR EVIDENCE FOR ANAMORPH DETERMINATION OFCORDYCEPS SINENSIS(BERK.) SACC.I.Relation Between Hirsutella sinensis and C.sinensis[J];MYCOSYSTEMA;2000-01
8 Wang Hong-Kai Zhang Tian-Yu** Zhang Meng (Systematic Mycology and Lichenology laboratory, Chinese Academy of Sciences. Beijing 100080;Department of Plant Pathology, Shandong Agricultural University, Shandong 271018; Biotechnology Institute, Agricult;APPLICATION OF SEQUENCING OF 5.8S rDNA, ITS1 AND ITS2 ON IDENTIFICATION AND CLASSIFICATION OF ALTERNARIA AT SEPECIES LEVEL[J];Mycosystema;2001-02
9 HUANG Bo YU Chun-Xiu CHEN Xiao-Lin FAN Mei-Zhen LI Zeng-Zhi (Department of Forestry, Anhui Agricultural University, Hefei,230036);CLASSIFICATION AND IDENTIFICATION OF SPECIES AND STRAINS IN PAECILOMYCES BY USING RAPD[J];Mycosystema;2002-01
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