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《Journal of Gansu Agricultural University》 2010-06
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Comparison of three DNA extraction methods from Botryosphaeria berengriana f. sp. piricola

SU Ping1,2,ZHOU Zeng-qiang2,ZHU Jian-lan1,HOU Hui2,WANG Li2(1.College of Prataculture,Gansu Agricultural University,Lanzhou 730070,China;2.Zhengzhou Fruit Research Institute,CAAS,Zhengzhou 450009,China)  
Three extraction methods,urea,benzyl chloride and SDS-CTAB,were applied to extract the genomic DNA from Botryosphaeria berengriana f.sp.piricola.The results showed that all the three methods were capable of extracting the genomic DNA of the pathogen.The SDS-CTAB method had the highest quality DNA extraction and showed the most efficient; however,consumed longest extraction time.The urea method was the simplest and most convenient procedure,but had a lower quality extraction.The benzyl chloride method had a lowest quality and the extracted DNA contained a lot of protein impurities.The rDNA ITS analysis and electrophoresis analysis showed that the DNA extracted by all the three methods has fairly good quality for DNA amplification and satisfied the requirements for rDNA ITS analysis of the Botryosphaeria berengriana f.sp.piricola.
【Fund】: 公益性行业(农业)科研专项(nyhyzx07-055)
【CateGory Index】: S436.611
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