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《Journal of Gansu Agricultural University》 2010-06
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Screening of oligosaccharide elicitors and its induced plastochrone on poplar callus

LI Dui-Shu1,JI Yu-liang1,HU Jing-jiang2,YANG Miao-miao3(1.Department of Biological Medicine Engineering,Shangluo University,Shangluo 726000,China;2.College of Life Sciences,Northwest A & F University,Yangling 712100,China;3.College of Forestry,Northwest A & F University,Yangling 712100,China)  
Oligosaccharide elicitors were made from Dothiorella gregaria,chitosan-oligosaccharides and plant cell walls(kelp,orange peel and banana peel) by degradation method,and were used to induce poplar callus,the oligosaccharide elicitors with stronger activity were screened out and its induced plastochrone were also studied.The results showed that poplar callus produced resistance to the Dothiorella gregaria after they were induced by seven elicitors,and three elicitors with stronger activity were selected out and their suitable induction density were ditermined.The induced resistant effect of elicitor A1,B and C3 was 54.8 %,34.7 % and 32.3 %,respectively,and their suitable induction density was 20 μg·mL-1,25 μg·mL-1 and 50 μg·mL-1,respectively.At the same time,induced plastochrone was screened as 48 hours.It indicated that the oligosaccharide elicitors could start and speed up expression of the resistant gene.Accompanied with the fast expression of the gene,the resistant metabolism in the plant was increased,and resistant products accumulated rapidly,the resistance of the poplar cell was also increased.
【Fund】: 国家“十五”科技攻关项目(2001BA501A09);; 陕西省教育厅2010年科学研究计划项目(2010JK522)
【CateGory Index】: S792.117
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