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《Journal of Gansu Agricultural University》 2013-03
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Ultrasonic-assisted extraction of collagen

YANG Meng-meng,GUO Zhao-bin,YU Qun-li,LI Ru-ren(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China)  
Bovine tendons of yak from Qinghai Province were used as the raw materials to extract collagen.A novel approach,ultrasonic-assisted pepsin method,for preparation of collagen was developed.The optimal ultrasonic-assisted treatment and enzyme dose of pepsin were explored using response surface methodology.The results indicated that the optimal extraction condition of collagen was based on ultrasonic-assisted treatment for 7.41 min at 366 W power followed by pepsin for 24 h at 4 ℃ with an enzyme dose of 1∶133.The extraction yield of collagen was 31.04%.Comparing with the traditional extraction method,the extraction yield improved by 10.69%.Therefore,the ultrasonic-assisted pepsin method could offer promising practical applications in industrial production of collagen.
【Fund】: 国家现代农业(肉牛牦牛)产业技术体系项目(CARS-38);; 国家公益性行业(农业)科研专项经费(201203009)
【CateGory Index】: TQ936.2
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