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《Chinese Journal of Light Scattering》 2004-01
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Immunoassay Using Surface-enhanced Raman Scattering

XU Shu-ping~1, XU Wei-qing~(1*), JI Xiao-hui~2, SONG Bo~1, ZHAO Bing~1, YUAN Hang~3, MA Lan~3, BAI Yu-bai~2 istry of Education; 2. College of Chemistry,Jilin University,Changchun,130021,P. R. China; 3. Yuda Monoclonal Antibody Technology Center,Yunnan University,Kunming,650091, P. R. China)  
An immunoassay based on surface-enhanced Raman scattering (SERS) and nanoparticle labeling by silver enhancement is described. Immunoreactions between immunogold colloids modified by p-mercaptobenzoic acid(MBA) and Hepatitis B virus surface antigen, which were captured by the antibody-assembilized chips, are detected by this sandwich structure after silver enhancement via SERS.
【Fund】: 国家自然科学基金资助项目;; 973项目(批准号:20273022 20375014 20173019 60271020 20073016;及2002AA302203)
【CateGory Index】: O433
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