Influence of ShenFu Injection Combined with Cytokines on the Induction and Generation of Dendritic Cells in the Patients with Chronic Myelogenous Leukemia
REN Lili;WEN Ming;JI Jing;ZANG Aimin;SONG Zizheng;XU Yan;LI Fang;WEI Yingfei;Affiliated Hospital of Hebei University;The Third Hospital of Hebei Medical University;
Objective: To observe the effects of ShenFu injection(SFI) in different concentrations combined with cytokines(GM-CSF/IL-4) on the maturation and the function of dendritic cells(DC) in the patients with chronic myelogenous leukemia(CML). Methods: Mononuclear cells were derived from CML patients, and induced into DC in vitro, SFI in different concentrations(ultimate concentrations: low concentration 9.38mg/mL; moderate concentration 37.50 and 75 mg/mL; high concentration 150 and 300 mg/mL crude drug concentration), GM-CSF/IL-4, SFI in different concentrations combined with cytokines were respectively added to culture systems, the obtained cells were identified by observing cellular morphology, the expressions of cellular surface molecules and the changes of cellular functions. Results: CML cells couldn't be induced and differentiated into DC by using SFI in vitro; there was phenotype and functional disorder existed in cytokines-induced CML-DC; while SFI added to the culture system which contained cytokines demonstrated dual-directional regulation to CML-DC: promoting maturation and inhibiting proliferation, high concentrations of SFI could inhibit the growth of DC, even cause cellular death, moderate concentration of SFI could obtain more mature CML-DC, it could improve the expressions of surface costimulatory molecules,the ability of stimulating lymphocytes proliferation and the contents of IL-12. Conclusion: SFI in appropriate density could affects the differentiation and maturation of CML-DC, it could change the expressions of immune molecules of DC cellular surface and enhance its functions, the optimal induction concentration of SFI is 75 mg/mL, SFI at the concentration of 75mg/mL combined with GM-CSF/IL-4 show synergistic action.
【CateGory Index】： R733.72