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Study of Photosynthetic Characteristics of Transgenic Barley Based on Reflectance of Single Leaf

SUN Cai-xia1,YUAN Fei1,ZHANG Yu-lan2,CHEN Zhen-hua2,CH EN Li-jun2,WU Zhi-jie2 1.College of Sciences,Northeastern University,Shenyang 110004,China 2.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016, C hina  
Analysis of leaf reflectance can provide a rich source of information a bout plant physiological process,surface properties of the leaf,as well as int ernal structure.In the present paper,the changes of reflectance spectra and sp ectral indices for transgenic barley and non-transgenic control variety based o n measurements of single leaf were studied.The results showed that the ratio spe ctra that calculated by dividing one reflectance spectrum of transgenic barley b y its' non-transgenic counterpart spectra were better to illustrate fine diffe re nces among spectra of leaves from different barley varieties.Ratio spectra vari ed between each pairs,but for most ratio spectrum indicated the reflectance dif ferences between transgenic barley and non-transgenic counterpart were most obv i ous in the red spectrum around the chlorophyll peak and red edge(650~700 nm) an d nearly identical in the green spectrum near 550 nm and within the near infrar ed region(beyond 750 nm).Across the entire calculated spectrum,the changes of reflectance within the region from 650 to 700 nm seem to be very sensitive t o respond to possible unintended effects of genetic modification.Six published reflectance indices,the red edge(λRE),the modified normalized difference veg e tation index(mND),the structure-independent pigment index(SIPI),the ratio o f reflectance in the red and green regions of the spectrum(RRed/R Green),the pho tochemical reflectance index(PRI) and the near-infrared reflectance at 800 nm (NIRR800) were used to make comparisons possible between species.The anal yses of changes of these indices indicated that they were highly dependent on growth st ages and barley varieties.Once indices described above have significant changes will mean that photosynthetic process,function and status of plants may be cha nged.
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