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《Neural Injury and Functional Reconstruction》 2012-03
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Expression of TREK-1 on Cultured Neuron and Astrocytes under Normal and Hypoxia Condition

WU Xiao▲,XIE Min-jie,TANG Rong-hua.▲Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China  
Objective:To investigate the expression of two-pore domain potassium channel—TREK-1 on primary cultured neurons and astrocytes and the temporal changes of TREK-1 expression in astrocytes under hypoxia insult.Methods: The cortical neurons and astrocytes were cultured.The expression of TREK-1 in cultured neurons and astrocytes were analyzed by double immunofluorescence staining.The temporal changes of TREK-1 expression in astrocytes under hypoxia insults were detected by Real-time PCR.Results: The TREK-1 immuno-reactivity was expressed on both the neurons and astrocytes under normal condition.During hypoxia insult,the TREK-1 expression on astrocyte increases at early phases and decreases gradually at late phase.Conclusion: The TREK-1 immunoreactivity is expressed on both the neurons and astrocytes.In astrocytes under hypoxia,there are temporal changes on TREK-1 expression.
【Fund】: 国家自然科学基金(No.30971007)
【CateGory Index】: R363
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1 Xiao WU1, Ronghua TANG 1, Yang LIU 1, Jingjiao SONG 2, Zhiyuan YU 1, Wei WANG 1, Minjie XIE 1 1Department of Neurology, 2Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Small RNA Interference-mediated Gene Silencing of TREK-1 Potassium Channel in Cultured Astrocytes[J];华中科技大学学报(医学英德文版);2012-06
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