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《International Journal of Laboratory Medicine》 2006-06
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Evaluation of four commercially available liquid culture kits for detecting mycoplasma

HUANG Jin-mei, ZHENG He-ping, WU Xing-zhong, et al.Guangdong Provincal Centre for Skin Disorders and STDs Prevention and Control, Guangzhou 510500, China  
Objective To compare the validity of four commercially available liquid medium kits for identify mycoplasma.Methods A toal of 90 specimens were collected from clinical patients with urogenital tract infection. Four liquid medium kits and one agar medium commercially available in China for the identify of mycoplasma were selected for the evaluation at the same condition.Results Agar medium was used as the gold standard. The sensitivity and specificity of ureaplasma urealyticum(Uu) after 24 hours incubation of IST(import), Kit1(China-made 1), Kit2(China-made 2) and Kit3(China-made 3) were 96% and 100%, 44% and 100%, 68% and 100%, 18% and 100% respectively. The sensitivity and specificity of mycoplasma hominis (Mh) after 48 hours of IST, Kit1, Kit2 and Kit3 were 95% and 100%, 65% and 100%, 2.5% and 100%, 0% and 100%, respectively. Comparison the sensitivity between IST and agar method, there was nosignificant difference (P0.05), but other 3 China-made kits were significant difference (P0.01) between other kits and agar method.Conclusion Comparied with the results of agar method, IST was well consistent, while the other 3 kits were poorly consistence. The funding indicated that the commercially available liquid medium kits for identifing mycoplasma should be re-evaluated of their validity before being applied for the clinical diagnosis.
【CateGory Index】: R446.5
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【References】
Chinese Journal Full-text Database 4 Hits
1 FAN Fanghua,XIAO Zhen,YU Xiaomei,et al.Clinical Laboratory of Zhejiang Hospital,Hangzhou 310013,China;Analysis and drug sensitivity test of mycoplasms in non-gonococcal urethritis patients[J];Clinical Education of General Practice;2010-01
2 Li Kan et al.The Central Laboratory Of Molecule,Medicine College of Yantze University,Jingzhou 434000,China;Experimentul Study on Detecting the Infection Case of Ureaplasma Urealyticum[J];Journal of Medical Research;2009-04
3 HUANG Jin-mei ZHENG He-ping PAN Hui-qing ZENG Wei-ying WU Xing-zhong LI Mei-ling XUE Yao-hua Guangdong Provincial Centre for Skin Diseases and STIs Control and Prevention,Guangzhou 510500,China;External quality assessment of STD laboratories in Guangdong Province[J];Chinese Journal of Dermatology;2007-05
4 JIANG Xin-liang(Dongyang People′s Hospital Weishan Branch,Dongyang,Zhejiang 322109,China);Clinical significance of real-time PCR in detecting ureaplasma urealyticum[J];Chinese Journal of Nosocomiology;2011-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Department of Laboratory, Wanbei Coal-electricity Group Hospital, Suzhou 234000, Anhui HUANG Feng, QIN Shu-guo, BIAN Qi-xia;Comparison of Three Different Methods for Detection of Candida Vaginitis[J];Journal of Anhui Health Vocational & Technical College;2009-02
2 ZHAO Jin~1,DAI Li~2,XUE Chen-xin~2,LIU Meng-jun~2(1.College of Life Science,Agricultural University of Hebei,Baoding 071000,China;2.Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding 071001,China);Selecting remedial drugs for witches' broom disease via tissue culture[J];Journal of Agricultural University of Hebei;2006-01
3 JIA Li-yan,LI Yan-ming,ZHANG Ying (College of Animal Science and Technology Shanxi Agricultural University,Taigu,Shanxi,030801, China);Estiblishment and Application on the PCR Detection Technique of Mycoplasma Contamination in Live Vaccine[J];Progress In Veterinary Medicine;2005-06
4 ZHU Ze-rong, LIU Xian-zhou, RAO Bang-zhong, TANG Ji-lu, BAI Rui-zhen, ZONG Run-zhi, LIU Wei-huang. Department of Microbiology.Medical school,Wuhan University,Wuhan 430071, China;Regulation of Vitamin C to the mICAM-1 on Cells Induced by the Lipid Associated Membrane Protein of Mycoplasma Pneumoniae[J];Hubei Journal of Preventive Medicine;2005-01
5 PAN Jie-ping, LU Ya-hua, Ma Ying-chun. Department of Rheumatology, The First People′s Hospital of Changzhou, Jiangsu 213003, China;The clinical analysis of the urinary mycoplasma infection in patients with systemic lupus erythematosus[J];Chinese Journal of Rheumatology;2004-12
6 Li Feng,Wang Jinghua,Zhang LihongDepartment of Clinical Medicine,Tangshan Institute of Technology,Tangshan,Hebei(063000);Treatment Effect of Azithromycin on Mycoplasma Pneumoniae Pneumonia with Humoral Immuno-function Modulation in Pediatric Patients[J];Journal of Radioimmunology;2008-02
7 XU Gang;Advantages of Application of Mycoplasma Verifying Agar in Detection of Genital Mycoplasmas[J];International Medicine & Health Guidance News;2006-15
8 ZHONG You tian,MA Lian lang (The Section of Pathogenic Biology,Gannan Medical College,Ganzhou,Jiangxi 341000);The Analysis of the Information of Mycoplasma Infection on Urogenital Tract and Drug Susceptibility[J];Journal of Gannan Medical College;2002-01
9 HUANG Yun ping, CHEN Hai zhou, CHEN Hua, HONG Liu, HE Yan (The Central Hospital of Zhanjiang, Zhanjiang 524037, China);Quantitative assessment of Ureaplasma urealyticum in infected patients and normal carriers[J];Chinese Journal of Infection Control;2004-02
10 MA Wen-ping.Tianjin Tiechang Hospital,Shexian 056404,China;Analysis of ureaplasma urealyticum culture and drug susceptibility[J];Foreign Medical Sciences;2005-08
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 GAO xia. (Laboratory Department of ShenZhen Municipal Second People's Hospital, Shenzhen 518035,Guangdong, P. R. China);Analysis of results in detecting the infection of Ureaplasma urealyticum by using PCR[J];China Tropical Medicine;2005-06
2 Ma Zhichao,Chang Gaifeng,Chen Canfeng,Yu Gaobing Sanitation and Antiepidemic Station in Baoan District of Shenzhen City,Shenzhen,518102 Clinical laboratory of the People′s Hospital of Xixiang,Baoan,Shenzhen,518102;Correlation Study of the Concentration of Mycoplasma and it′s Clinical Symptoms[J];Journal of Tropical Medicine;2002-03
3 CHAO Zhi-xiang,ZHU Xi-chong.(Department of Dermato-Venereology,Zhejiang Taizhou Hospital,Taizhou,Zhejiang 318050,China);Study on drug resistance of ureaplasma uralyiam and mycoplasma hominis[J];Journal for China AIDS/STD;2004-02
4 ZHENG He-ping,DIAO Jian-xing,CHEN Yong-feng, et al.(Guangdong Provincial Centers for Skin Diseases and STIs Control and Prevention,Guangzhou, Guangdong 510500,China);Study of standardized STDs laboratories and quality control systems in Guangdong Province.[J];Journal for China AIDS/STD;2005-02
5 ZHANG Yong-le,ZHANG Song-ping,MA Lan,SHI Dai-qiang,ZHEN Ai-mei;Comparism of Four Kinds of Methods in Detection for Early Syphilis Infection[J];The Chinese Journal of Dermatovenereology;2008-02
6 HUANG Jin-mei ZHENG He-ping PAN Hui-qing ZENG Wei-ying WU Xing-zhong LI Mei-ling XUE Yao-hua Guangdong Provincial Centre for Skin Diseases and STIs Control and Prevention,Guangzhou 510500,China;External quality assessment of STD laboratories in Guangdong Province[J];Chinese Journal of Dermatology;2007-05
7 WANG Li-ping,ZI Jie,YI Hui(Shenzhen Futian Women and Children Health Center,Shenzhen,Guangdong 518026,China);Ureaplasma urealyticum and Mycoplasma hominis among Female Patients with Urogenital Tract Infection and Their Culturing and Drug Resistance[J];Chinese Journal of Nosocomiology;2007-05
8 ZHANG Yong-le,ZHANG Song-ping,MA Lan,XU Dai,ZHENG Aai-mei,SHI Dai-qiang(Hangzhou Sixth People′s Hospital,Hangzhou,Zhejiang 310014,China);Detection of Chlamydia trachomatis and Ureaplasma urealyticum by Fluorescence Quantitative PCR[J];Chinese Journal of Nosocomiology;2009-10
9 CHENG Gang, HE Yun-Shao, ZHOU Xin- Yu et al. Da'an Gene Diagnostic Center of Zhongshan University, Guangzhou 510089;Development of hepatitis C virus(HCV) fluorescence PCR (F-PCR) diagnostic kit and its clinical trial comparing with immunological method[J];Chinese Journal of Immunology;2002-07
10 LIAO Qi-hong,LIANG Zhi-dong,MEN Xiao.(The People′s Hospital of Liuzhou,545001,China);Detection of Chlamydoza trachomatis and Ureaplasma urealyticum by fluoroqenic quantitative polymerase chain reaction in endocervices of women.[J];Chinese Journal of Birth Health & Heredity;2006-05
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 CAO Wen-ling, LIANG Yan-hua, YE Xing-dong, LI Ping, LI Xiao-dong, WU De-biao, HUANG Xue-mei, TAN Shao-kai, ZHANG Li, BI Chao(Guangzhou Institute of Dermatology, Guangzhou 510095, China);Evaluation on External Quality of Chlamydia trachomatis Measurement in Guangzhou in 2007[J];Journal of Tropical Medicine;2009-07
2 FAN Fanghua,XIAO Zhen,YU Xiaomei,et al.Clinical Laboratory of Zhejiang Hospital,Hangzhou 310013,China;Analysis and drug sensitivity test of mycoplasms in non-gonococcal urethritis patients[J];Clinical Education of General Practice;2010-01
3 BI Chao,CAO Wen-ling,LIN Lu-yang,et al.(Guangzhou Municipal Institute of Dermatology,Guangzhou,Guangdong 510095,China);An analysis of external quality assessment of STD laboratories in Guangzhou in 2009[J];Chinese Journal of AIDS & STD;2010-05
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