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《International Journal of Laboratory Medicine》 2009-06
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Expression of Bcl-2 and Caspase-3 protein in osteosarcoma and its clinical significance

LIU Bing.De- partment of Clinical Laboratory,the 324th Hospital of PLA,Chongqing 400020,China  
Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma, and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software. Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3%and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking gradeⅠto gradeⅢrespectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures. Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma; however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.
【CateGory Index】: R738.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 XUE Xiao-jie,LIU Jia-jia,XIE Hua-fu (Department of Clinical Laboratory,Huangshi Central Hospital,Huangshi 435000,China);Association of expression of Mcl-1 and Puma gene in cervical carcinomas[J];International Journal of Laboratory Medicine;2010-08
【Citations】
Chinese Journal Full-text Database 2 Hits
1 LI Zheng. People Hospital of Liaoning Province ( Liaoning Shenyang,110015);Expression and clinical significance of P53.Bcl-2 and Bax protein in human osteosarcoma[J];China Journal of Orthopaedics and Traumatology;2002-04
2 GAO Da-xin,LU Gang,ZHANG Hai-yan,et al.Department of Osteology,The First Affiliated Hospital,China Medical University,Shenyang 110001,China;The relationship between expression of Survivin mRNA and apoptosis in human osteosarcoma[J];Chinese Journal of Experimental Surgery;2004-03
【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 Liu Lin (Department of obstetrics and gynecology, Fushun central hospotal, Liao Ning, 113006, China);Change of serum of prostaglandin E2 and bcl-2 in patients with ectopic tubal pregnancy[J];Jiangxi Medical Journal;2006-05
2 GAO Yong-jun,WANG Tao,LI Ran,LI Bao-chen,PU Jun,XU Wei(1)Dept.of Neurosurgery,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101;2)Dept.of Neurosurgery,People's Hospital of Pu'er,Pu'er Yunnan 665000,China);The Effects of Selective Cerebral Ultra-Profound Hypothermia on Bc1-2 and Bax Expression in Brain Tissues of Monkeys after Occlusion of Cerebral Blood Flow under Different Time Limits[J];Journal of Kunming Medical University;2012-01
3 Zhao Jun Qinghai Provincial People's Hospital(810007);4-O-Amino-Phenol-4'-Denmethylepidophylotxin Ether and K562 Cell Apoptosis:A Comparative Study[J];Qinghai Medical Journal;2010-08
4 Liu Ying1, Chen Chen2, Wu Wei-kang3, Zhao Dan-yang3 1Department of Pathophysiology, Guangdong Pharmacological College, Guangzhou 510224, Guangdong Province, China; 2Department of Clinical Laboratory, the 421 Hospital of Chinese PLA, Guangzhou 510318, Guangdong Province, China; 3Institute of Integrated Traditional Chinese and Western Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China;Protective effects of delayed preconditioning against cell apoptosis caused by myocardial ischemia reperfusion[J];Chinese Journal of Clinical Rehabilitation;2006-20
5 Wei YT,Du JF,Zhu JL,Luo B. Department of Thoracic and Cardiovascular Surgery,First Hospital Affiliated to the Medical College of Shihezi University,Shihezi 832008,Xinjiang Uygur Autonomous Region,China;Effects of regional ischemic preconditioning on myocardial cell apoptosis and expression of Bcl-2 and Bax protein in rabbits with short-term ischemic reperfusion[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-21
6 LIU Ying,CHEN Chen,WU Weikang,et al. Department of Pathophysiology of Guangdong Pharmacological College, Guangzhou,510224;Mechanism of delayed preconditioning protects myocardial cell against apoptosis: investigating mitochondria signal pathway of apoptosis[J];Chinese Journal of Rehabilitation Medicine;2006-05
7 Lu Gang Gao Da-xin Zhang Hai-yanDepartment of Orthopaedics,the First Affiliated Hospital of China Medical University,Shenyang;An Empirical Study on Survivin Gene Expression in Blocking Osteosarcoma Cells by Antisense Technology[J];Chinese Journal of Clinical Oncology;2004-21
8 ZHAO Jun.The People's Hospital of Qinghai Province,Xi'ning 810007,China;Study of effects of 4-o-aminophenol-4' - demethylepipophyliotoxin ester(ODE) on K562 cell apoptosis[J];Medical Innovation of China;2010-26
China Proceedings of conference Full-text Database 1 Hits
1 JI Yu-bin1,2,3,LIU Guang-da *1,YU Lei1,2,3,LI Hai-jiao 1,YANG Hai-fan1,PANG Lin-lin1(1 Center of Research and Development on Life Sciences and Environmental Sciences,Harbin University of Commerce,Harbin 150076,China;2.Institute of Materia Medica and Postdoctoral Programme of Harbin University of Commerce,Harbin 150076,China;3.Engineering Reseach Center of Natural Anticancer Drugs,Ministry of Education);Development of Survivin and Tumor Research[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YU Xiao-Lin, LIU Hong, CHEN Wan-Ping, CHENG Peng-YuanOutpatient Department, Fourth Military Medical University, Xi'an 710033, China;Effects of recombinant Caspase-3 on human laryngocarcinoma cell line Hep-2 through coexpression with GFP[J];Journal of The Fourth Military Medical University;2005-21
2 LIN Hui-min,ZHANG Yu-wen,LIU Xing-han(Department of Biochemistry,Harbin Medical University,Harbin 150081,China);Effect and mechanism of puma gene on the apoptosis of HeLa cell in vitro[J];Journal of Harbin Medical University;2008-01
3 ZHANG Shi-quan1,XIAO De-ming1,ZHU Yu-hua1,GUAN Hong2,LI Wei1,TAN Ji-feng11Department of Orthopeadics;2Depatrment of Pathdogy the Second People's Hospital of Shenzhen,Shenzhen 518035,China.;Protein expression of p53 in osteosarcoma and the correlation with clinical features and prognosis[J];Journal of Modern Oncology;2011-01
4 LI Zhi-Hui,LI Hui-Min Department of Hematology,Yunnan Second People Hospital,Kunming 650021,Yunnan Province,China;Progress of Study on Relationship between Mcl-1 Gene and Hematologic Malignansies——Review[J];Journal of Experimental Hematology;2009-02
5 ZHANG Ke-jun, LI De-chun, ZHU Xin-guo, ZHU Dong-ming, ZHAO Zhi-hong. Department of General Surgery, The First Affiliated Hospital, Suzhou University, Suzhou 215006, JS, China;PUMA gene inhibits the growth of PC-3 Pancreatic carcinoma cells[J];Chinese Journal of Pancreatology;2006-06
6 JIANG Dong1,ZHENG Shi-ying1,CHEN Suo-cheng2.(1.Department of Cardiothoracic surgery,First Affiliated Hospital of Soochow University,Suzhou 215006,China;2.Department of Cardiothoracic Surgery,First People's Hospital Affiliated to Jiangsu University,Zhenjiang 212002,China);Research Progress of Whole Body Hyperthermia and Apoptosis of Tumor Cells[J];Medical Recapitulate;2008-01
7 CHEN Yun-ru,WANG Yu-ming,DUAN Yong.(Department of Laboratory,First Affiliated Hospital of Kunming Medical College,Kunming 650032,China);Promoting Apoptotic Effect of PUMA and Tumor Therapy[J];Medical Recapitulate;2008-24
8 LI Zheng. People Hospital of Liaoning Province ( Liaoning Shenyang,110015);Expression and clinical significance of P53.Bcl-2 and Bax protein in human osteosarcoma[J];China Journal of Orthopaedics and Traumatology;2002-04
9 GAO Da-xin,LU Gang,ZHANG Hai-yan,et al.Department of Osteology,The First Affiliated Hospital,China Medical University,Shenyang 110001,China;The relationship between expression of Survivin mRNA and apoptosis in human osteosarcoma[J];Chinese Journal of Experimental Surgery;2004-03
10 GAO Da-xin*,ZHANG Hai-yan,L Gang,et al.*Department of Orthopaedics,The First Hospital of Qinhuangdao,Qinhuangdao 066000,China;Survivin antisense oligonucleotide combined with DDP in the treatment of nude mice bearing human osteosarcoma xenograft[J];Chinese Journal of Experimental Surgery;2005-08
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 YU Xian-xiang1,2,ZHANG Lin-jie1,XIE Ben-jun3(1.Anhui Medical University,Hefei,Anhui 230032;2.Chaohu Vocational and Technical College; 3.The First People's Hospital of Chaohu,Chaohu,Anhui 238000);Significance of Mcl-1 expressions in gastric cancer by tissue microarray[J];Anhui Medical and Pharmaceutical Journal;2012-05
2 LV Nanqian,FEI qing,ZHANG Qiyun,LOU Guoxiang.Department of Orthopaedics,Liqun Hospital,Shanghai 200333,China;The influence of chemokine receptor CXCR4 gene on the proliferation and migration of human osteosarcoma cells[J];Chinese Orthopaedic Journal of Clinical and Basic Research;2012-05
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