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《International Journal of Laboratory Medicine》 2010-03
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Olfactory ensheathing cell induce the maturation of dendritic cell

WU Zhen,WEI Dong,WEI Xiao-mou,et al. (Biotechnological Institute,The Fourth Hospital Affiliated to Guangxi Medical University,Nanning 645005,China)  
Objective To determine ability of OEC that induces the maturation of DC in vitro.Methods In this study,OEC and DC were prepared from aborted human embryo and human peripheral blood of healthy donors,respectively.DC was incubated with OEC,in other experiments,DC and OEC were separated by culture inserts to prevent direct cell-cell contact or cultured alone in 24-well plates in 5%CO2 incubator at 37℃.After 48 h,TNF-αand IL-12p40 in the suspension were detected by ELISA,the level of CD40,CD80,CD86 and HLA-DR on DC were determined by flow cytometry.Results By 12 d,the purity of OEC was up to 90%with NGFRp75 immunostaining.Expression of NGF,BDNF,GDNF were detected by RT-PCR.The level of TNF-,IL-12p40, CD40,CD80,CD86 and HLA-DR were enhanced by induction of OEC,comparing direct cell-cell contact culture,the higher level was detected in nondirect cell-cell contact culture.Conclusion OEC can induce the maturation of DC,cell-cell contact decrease the activation of DC.
【Fund】: 广西科学基金(0640193);; 广西“新世纪十百千人才工程”专项资金;; 广西医疗卫生重点科研课题(20055);; 柳州市科技攻关项目(2006031101)
【CateGory Index】: R329
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