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《International Journal of Laboratory Medicine》 2010-06
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Analysis of Positive Blood Donors from NAT Screening:Comparison with ELISA Tests

ZEN Jin-feng,LI Huo,XU Xiao-xun,et al. (The Clinical Laboratory of Shenzhen Blood Centre 518035,China)  
Objective To improve the safety in blood transfusion,a comparison between positive nucleic acid test(NAT) screening results and ELISA test results was carried out to study how to elevate the test sensitivity in ELISA blood screening. Methods Apply NAT test in 8-sample minipool to 55 499 donor plasma samples that were collected from January 1,2008 to March 31,2009 and were non-reactive in ELISA blood screening tests.Analyze the ELISA results from NAT positive samples.Results Among 55 499 ELISA non-reactive samples,we detected 11 HBV-DNA positive,1 HCV-RNA positive,none HIV-RNA positive. Conclusion NAT and ELISA complement to each other in 3 aspects.1) Window-period false-negative is the main cause in current ELISA false-negatives.2)Immuno silent infections,viral mutations,viral subtypes can reduce the analytical performance of antibodyantigen immunoreaction based ELISA test.3)The establishment of "gray-zone" in ELISA result interpretations can decrease the false-nagatives caused by sensitivity of the tests,however,it does not resolve the false-negatives from viral mutations,subtypes and "window periods".
【Fund】: 深圳市科技局立项课题(200803116)
【CateGory Index】: R457.1
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 WU Yuqing,YANG Zhongsi,ZHAO Lin,et al.Qingdao Blood Center,Qingdao 266071,China;Study of nuclei-acid-test(NAT) among volunteer blood donors from Qingdao area[J];Chinese Journal of Blood Transfusion;2008-11
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 LIU Lidong,LI Chao,GAO Yu,et al.Shanghai Blood Center,Shanghai 200051,China;Primary research of the importance of ALT testing on the safety of blood donor screening system[J];Chinese Journal of Blood Transfusion;2009-07
2 JIANG Ni-zhen,HUANG Cheng-yin,XIAO Jian-yu,et al.Blood Center of Jiangsu Province,Nanjing 210042;Individual Donation Nucleic Acid Test Using Procleix TIGRIS System[J];Journal of Clinical Transfusion and Laboratory Medicine;2010-03
3 FAN Jia-cheng,GAO Mei-hua.Qingdao University Medical College,Qingdao 266071,China;Survey of the Residual Risks of Screening HBV and the Test Mode in Taian[J];Journal of Community Medicine;2010-09
4 TANG Jiu-ge1,DING Jian-bing1,GOU Jing2,QIAO Yan-hui2,YUAN Xiao-hua2,HE Zhi2,GUO Wei-peng2 (1.College of Basic Medicine,Xinjiang Medical University,Urumqi 830054,China;2.Blood Center,Xinjiang,Urumqi 830000,China);Comparing PCR coupled with blot-hybridization and enzyme linking to FQ-PCR to detect HBV[J];Journal of Pathogen Biology;2010-01
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 WANG Lianghua,YE Xianlin,SHANG Guifang,et al.Shenzhen Blood Center,Shenzhen 518035,China;NAT testing for HBV,HCV,HIV on donors' samples negative by ELISA tests[J];Chinese Journal of Blood Transfusion;2005-04
2 HU Zhaoping,YAO Ping,WANG Baolong,et al.Department of Transfusion of Anhui Provincial Hospital, Hefei 230001;Application and analysis of feasibility to FQ-PCR in blood screening[J];Journal of Clinical Transfusion and Laboratory Medicine;2002-01
3 REN Fu-rong*, LIU Chang-li, Lü Qiu-shuang, LI Hui, ZHANG Ping, GAO Dong-ying, GAO GUO-jing, MA Guo-dong, ZHAO Hai-yan, ZHUANG Hui *Beijing Red Cross Blood Center, Beijing 100088, China Corresponding author:ZHUANG Hui, Email:Zhuanghui@publia.bjcninfo.net;Nucleic acid testing in sero-negative blood donors[J];Chinese Journal of Laboratory Medicine;2004-09
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