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《International Journal of Laboratory Medicine》 2011-01
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Comparison of effects on dendritic cells maturation stimulated by TNF-alpha and lip polysaccharide in patients with tuberculosis

Huang Jiayu1,Zhao Yan2,Song Xiuyu1,Ye Zhizhong3,Liao Yinhui1(Department of Clinical Laboratory,Xiamen First Hospital Affiliated to Fujian Medical University,Xiamen 361003,China)  
Objective To evaluate the effects on dendritic cells maturation stimulated by TNF-alpha and lip polysaccharide in patients with tuberculosis.MethodsThe peripheral blood mononuclear cells were separated by using density gradient centrifugation and were cultured by addition of rhGM-CSF and rhIL-4.TNF-alpha and lip polysaccharide were added on the 7th day and total RNA was extracted on the 11th day.Expressions of IL-12p40,IL-12p35 and CCR7 mRNA were detected by using real time PCR,while amounts of IL-12 in the supernatant were detected by ELISA tests.Resultsdendritic cells expressed a higher IL-12p40,IL-12p35,CCR7 mRNA when stimulated by lip polysaccharide than they were when stimulated by TNF-alpha(P0.05);ELISA tests showed higher IL-12 amounts in lip polysaccharide-stimulation group than it was in TNF-alpha-stimulation group.Conclusionwith the higher stimulation ability,lip polysaccharide showed to be a better stimulator for the dendritic cells of tuberculosis patients.
【Fund】: 福建省卫生厅青年课题(2008-2-40 2009-2-74)
【CateGory Index】: R52
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHUANG Yi-hong,LI Chun-mei.Department of Laboratory,the First Affiliated Hospital of Medical College of Shantou University,Shantou 515041,China;A study of generation of monocyte-derived dendritic cell from peripheral blood and inducement of immunity of bresat neoplasm[J];International Journal of Laboratory Medicine;2006-01
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 WU Zhen,WEI Dong,WEI Xiao-mou,et al. (Biotechnological Institute,The Fourth Hospital Affiliated to Guangxi Medical University,Nanning 645005,China);Olfactory ensheathing cell induce the maturation of dendritic cell[J];International Journal of Laboratory Medicine;2010-03
2 JIANG Peng-jun,ZHU Xue-jun,SUN Xue-mei(Department of Hematology,Jiangsu Province Hospital of Traditional Chinese Medicine,Affiliated with Nanjing University of Chinese Medicine,Nanjing 210029,China);Detection of dendritic cell subsets in peripheral blood of the patients with aplastic anemia[J];International Journal of Laboratory Medicine;2010-12
3 LIU Yun-jiang~1, ZHANG Jian-li~1, SHAN Bao-en~ 2* ~1Department of Surgery, ~2Research center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China;Induction of specific CTL by DC from axillary lymph node of patients with breast cancer[J];Chinese Journal of Cellular and Molecular Immunology;2007-01
4 LIU Yun-Jiang,ZHANG Jian-Li,SHAN Bao-En.The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Study on DC loaded with freeze-thrawing antigen from breast cancer cells to the specific cytotoxicity of CTL in vitro[J];Chinese Journal of Immunology;2007-08
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhu Xuejun , Cao Xuetao , Yu Yizhi et al ( Department of Immunology, Second Military Medical University, Shanghai 200433);Generation of Dendritic Cells from Human Peripheral Blood[J];Chinese Journal of Cancer Biotherapy;1997-04
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