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《International Journal of Laboratory Medicine》 2011-01
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Comparison of effects on dendritic cells maturation stimulated by TNF-alpha and lip polysaccharide in patients with tuberculosis

Huang Jiayu1,Zhao Yan2,Song Xiuyu1,Ye Zhizhong3,Liao Yinhui1(Department of Clinical Laboratory,Xiamen First Hospital Affiliated to Fujian Medical University,Xiamen 361003,China)  
Objective To evaluate the effects on dendritic cells maturation stimulated by TNF-alpha and lip polysaccharide in patients with tuberculosis.MethodsThe peripheral blood mononuclear cells were separated by using density gradient centrifugation and were cultured by addition of rhGM-CSF and rhIL-4.TNF-alpha and lip polysaccharide were added on the 7th day and total RNA was extracted on the 11th day.Expressions of IL-12p40,IL-12p35 and CCR7 mRNA were detected by using real time PCR,while amounts of IL-12 in the supernatant were detected by ELISA tests.Resultsdendritic cells expressed a higher IL-12p40,IL-12p35,CCR7 mRNA when stimulated by lip polysaccharide than they were when stimulated by TNF-alpha(P0.05);ELISA tests showed higher IL-12 amounts in lip polysaccharide-stimulation group than it was in TNF-alpha-stimulation group.Conclusionwith the higher stimulation ability,lip polysaccharide showed to be a better stimulator for the dendritic cells of tuberculosis patients.
【Fund】: 福建省卫生厅青年课题(2008-2-40 2009-2-74)
【CateGory Index】: R52
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