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《International Journal of Laboratory Medicine》 2011-14
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Construction of mouse models of type 1 diabetes mellitus

Tan Xiao1,2,Xiao Jianhua1(1.Nanhua University Pathogenic Biology Research Institute,Hengyang Hunan 421001,China;2.Shaoyang Medical College,Shaoyang Hunan 422000,China)  
Objective To construct mouse models of type 1 diabetes mellitus(T1DM) to provide the evidences for researches on the pathogenesis and therapeutic method.Methods Mouse models of T1DM were induced through enterocoelia limosis injection of streptozotocin(MLDSTZ).64 male BALB/c mice were randomly divided into three groups,including model group,negative controls and normal controls.Model mice received intraperitoneal injection continuously for 5 days with STZ solution,negative controls received intraperitoneal injection with citric acid buffer,and normal controls group were not treated.Then blood and urine sugar were detected after the injection,and changes of pancreas of the mice were observed.Results Model mice induced by STZ were obviously with polydipsia,polyphagia,hyperdiuresis and loss of body weight,compared with controls.3 weeks after the last time of STZ injection,model mice were positive with urine sugar,but controls were negative.After 4 weeks,fasting blood glucose level of model mice was higher than controls(P0.01).Model mice were with different degrees of islet atrophy,beta cell vacuoles degeneration and decrease,and different degrees of islet inflammation.Conclusion Mouse model of T1DM,constructed by using MLDSTZ,were stable.This result prove that the method is reliable.
【CateGory Index】: R-332
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