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《International Journal of Laboratory Medicine》 2011-14
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Construction of mouse models of type 1 diabetes mellitus

Tan Xiao1,2,Xiao Jianhua1(1.Nanhua University Pathogenic Biology Research Institute,Hengyang Hunan 421001,China;2.Shaoyang Medical College,Shaoyang Hunan 422000,China)  
Objective To construct mouse models of type 1 diabetes mellitus(T1DM) to provide the evidences for researches on the pathogenesis and therapeutic method.Methods Mouse models of T1DM were induced through enterocoelia limosis injection of streptozotocin(MLDSTZ).64 male BALB/c mice were randomly divided into three groups,including model group,negative controls and normal controls.Model mice received intraperitoneal injection continuously for 5 days with STZ solution,negative controls received intraperitoneal injection with citric acid buffer,and normal controls group were not treated.Then blood and urine sugar were detected after the injection,and changes of pancreas of the mice were observed.Results Model mice induced by STZ were obviously with polydipsia,polyphagia,hyperdiuresis and loss of body weight,compared with controls.3 weeks after the last time of STZ injection,model mice were positive with urine sugar,but controls were negative.After 4 weeks,fasting blood glucose level of model mice was higher than controls(P0.01).Model mice were with different degrees of islet atrophy,beta cell vacuoles degeneration and decrease,and different degrees of islet inflammation.Conclusion Mouse model of T1DM,constructed by using MLDSTZ,were stable.This result prove that the method is reliable.
【CateGory Index】: R-332
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Chinese Journal Full-text Database 1 Hits
1 XIA Xuan,WENG Jian-ping. Department of Endocrinology,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Streptozotocin and its role in diabetic model[J];International Journal of Internal Medicine;2009-09
Chinese Journal Full-text Database 10 Hits
1 FU Xiu-mei MA Bao-jun Zhao Li-jun et al (Chengde Medical College,Hebei Chengde 067000,China);EFFECTS OF SERICINE ON BLOOD SUGAR OF TYPE 2 DIABETES MELLITUS RAT MODEL[J];Journal of Chengde Medical College;2008-04
2 JIANG Jing1,WU Chunwei2,LI Xing1,LUO Zhaoxun1,HAN Fang1,PAN Wei1,YANG Guozhen1(1.Department of Clinical Laboratory Science,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Department of Outpatient Clinic,the Affiliated Hospital of Guiyang Medical College,Guiyang 550001,Guizhou,China);A Study on Establishment of Gestational Diabetes Rat Model with Different Doses of Streptozotocin[J];Journal of Guiyang Medical College;2010-03
3 LIU Xin,FAN Li-feng.Endocrine Department,the General Hospital of PLA,Beijing 100853,China;Investigation of patient's satisfactory rate on health education of diabetes mellitus[J];Modern Nursing;2006-08
4 YAO Xiao-jun,LAN Yuan-yuan.No.196 Hospital of the People's Liberation Army,Zhanjiang Guangdong 524039,China;Relationship between Streptozotoci Induced Impaired Glucose Tolerance and Its Dosage in the Neonatal Rats[J];Military Medical Journal of South China;2010-05
5 ZHU Zhang-ling,MA Qian-qian,CHEN Hong-yu,LI Ying(Ophthalmology Department,Jingzhou Hospital Affiliated Tongji Medical College of Huazhong University of Science and Techololgy,Jingzhou 434020 Hubei Province,China);Problems and Countermeasures of Screening and Controling of Diabetic Retinopathy[J];Nursing Journal of Chinese People's Liberation Army;2011-03
6 DAI Xiao-si,KANG Jian,WAN Rui-han,RONG Yang-ling,WANG Ting(Department of Anatomy,North Sichuan Medical College,Nanchong Sichuan 637007,China);Establishment of type 1 diabetes mellitus animal model induced by alloxan[J];Journal of Regional Anatomy and Operative Surgery;2011-01
7 ;实验性糖尿病大鼠建模常用方法[J];Science & Technology Information;2011-21
8 TANG Qiu.Laboratory Animal Center of The Second Military Medical University,Shanghai 200433,China;Preparation and evaluation of rat model of type 2 diabetes[J];Sichuan Medical Journal;2011-04
9 Pang Yue,Yao Yinfang(First People's Hospital of Chuzhou City Anhui Province,Anhui 239000 China);Influence of nursing intervention on psychological insulin resistance patients[J];Chinese Nursing Research;2007-21
10 LI Qing, XU Yun, GUO Ya-ying(Affiliated Hospital of Yuncheng Public Health School,Yuncheng,Shanxi 044000,China);Diabetic Retinopathy's Therapy and Nurse[J];Journal of Practical Medical Techniques;2006-10
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 DUAN Bin-hong, ZHAN Xiao-rong, YIN Hui-qing, et al. (Department of Endocrinology, Heilongjiang Provincal Hospital, Harbin 150036, China);Determination of Insulin Gene Promoter Mutant in Diabetic Patients and Its Clinical Significance[J];Labeled Immunoassays and Clinical Medicine;2004-02
2 Wang Ping 1) Yan Fang 1) He Zhongxiao 1) Ni Qing 2) Liu Ximing 2) Lin Lan 2) ( 1)College of Life Science, Beijing Normal University, 100875, Beijing, China; 2)Guanganmen Hospital, Institute of Chinese Traditional Med;THE CHANGES OF GENOME DNA METHYLATION OF KIDNEY AND PANCREAS FROM DIABETIC RAT[J];Journal of Beijing Normal University(Natural Science);2001-02
3 Song Huiping;He Yuan;Tang Yong(Department of Biochemistry, Hunan Medical University)Han Xiuyun(Department of Internal Medicine,Xiangya Hospital,Munan Medical University;HYPERGLYCEMIA AND ALDOSE REDUCTASE ACTIVATION[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1994-06
4 AI Jing,GUO Jian,ZHANG Yong chun, et al (Department of Pharmacology,Harbin Medical University,Harbin 150086,China);Study on the diabetic model induced by alloxan in Wistar rats[J];Journal of Harbin Medical University;2001-02
5 LIU Ke-yu,WANG Chun-hua,DU Zhi-min(Institute of Clinical Pharmacy and Drug,The Second Clinical College,Harbin Medical University,Harbin 150081,China);Study on the diabetic model induced by streptozocin in Wistar rats[J];Journal of Harbin Medical University;2006-04
6 HE Xue ling, YIN Hai lin(Laboratory Animal Center of Sichuan University, Chengdu 610041, Sichuan Province,China);EFFECTS OF COPYING DIABETES MELLITUS RAT MODEL BY DIFFERENT DOSES AND INJECTING METHODS WITH ALLOXAN[J];Sichuan Journal of Zoology;2003-04
7 SUN Gui-ju, WANG Shao-kang, ZHANG Xiao-qiang, ZHANG Yong, ZHANG Lin, SUN Zi-lin (Dept. of Nutrition and Food Hygiene, School of Public Health; Section of endocrinology, School of Clinical Medicine, Southeast University, Nanjing 210009,China);Establishment of Diabetes Ⅱ Animal Model and Study on indexes Related to Diabetic Complication[J];Shanghai Laboratory Animal Science;2003-02
8 SHUANG Wei-bing, WANG Zhi-hui, WANG Dong-wen, et al (Dept of Urology, First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, China);Development and validation of the rat model with type 2 diabetes mellitus[J];Journal of Shanxi Medical University;2005-03
9 Wang Kaifu;Li Minzhen;Ye Wangyun et alInstitute of Integrated Traditional Chinese and Western Medicine,Tongji Medical University,Wuhan 430030;Influence of Doses of Alloxan and Sex on the Development of Diabetic Model in Rabbits[J];Acta Universitatis Medictnae Tangji;1994-03
10 CHEN Qiu, XIA Yongpeng, QIU ZongyinDepartment of Pharmacology, Chongqing Medical University, Chongqing 400016,China;Establishment and Evaluation of Rat Models of Type 2 Diabetes Mellitus[J];Tianjin Medical Journal;2006-01
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