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《International Journal of Laboratory Medicine》 2013-19
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Analysis of the nucleic acid amplification technique applied in the screening of donated blood

Wu Gongxiao;Lai Fuchun;Shi Dongmei;Zhang Weifang;Wu Rong;Kuang Jieping;The Blood Testing Department of Zhuhai Centre Blood Bank;  
Objective To evaluate the practical application of the nucleic acid amplification techniques(NAT)to the screening of donated blood.Methods Negative samples by ELISA test were detected with HBV/HCV/HIV NAT reagent.Pools of 8samples were used for NAT testing and those samples in initial positive reactive pools were further detected.Results Out of 33 714ELISA negative samples,5HBV DNA positive and the positive rate was 0.015%;neither HCV RNA nor HIV-1RNA positive samples was detected.Conclusion It is necessary to apply NAT to blood screening for blood safety.
【CateGory Index】: R446.6
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