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《International Journal of Laboratory Medicine》 2013-19
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Analysis of the nucleic acid amplification technique applied in the screening of donated blood

Wu Gongxiao;Lai Fuchun;Shi Dongmei;Zhang Weifang;Wu Rong;Kuang Jieping;The Blood Testing Department of Zhuhai Centre Blood Bank;  
Objective To evaluate the practical application of the nucleic acid amplification techniques(NAT)to the screening of donated blood.Methods Negative samples by ELISA test were detected with HBV/HCV/HIV NAT reagent.Pools of 8samples were used for NAT testing and those samples in initial positive reactive pools were further detected.Results Out of 33 714ELISA negative samples,5HBV DNA positive and the positive rate was 0.015%;neither HCV RNA nor HIV-1RNA positive samples was detected.Conclusion It is necessary to apply NAT to blood screening for blood safety.
【CateGory Index】: R446.6
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Chinese Journal Full-text Database 4 Hits
1 Wang Xun,Zheng Lan,Zhang Xi,et al. Shanghai Blood Center, Shanghai 200051, China;Preliminary experiences of nucleic acid testing in blood screening in Shanghai[J];Chinese Journal of Blood Transfusion;2003-03
2 ZENG Jin-feng,YE Xian-lin,MA Lan,et al.Shenzhen Blood Center,Shenzhen 518035,China;Complementarity of ELISA and nucleic acid amplification test in blood screening[J];International Journal of Laboratory Medicine;2008-08
3 YE Xian-lin~1,SUN Shu-jun~1,RONG Ying~2,LI Huo~1,XU Xiao-xuan~1,ZHANG Hong~1,GU Xing-hui~1(1 Shenzhen Blood Center Collaborative Laboratory for Nucleic Acid Test of National Center for Clinical Laboratories,Shenzhen 518035,China;2 Shenzhen Blood Center,Shen zhen 518000,China);Establishment and application of domestic entirely automatic system of nucleic acid amplification testing in blood screening[J];Chinese Journal of Infection Control;2010-05
4 HU Zhaoping,YAO Ping,WANG Baolong,et al.Department of Transfusion of Anhui Provincial Hospital, Hefei 230001;Application and analysis of feasibility to FQ-PCR in blood screening[J];Journal of Clinical Transfusion and Laboratory Medicine;2002-01
Chinese Journal Full-text Database 10 Hits
1 YE Xianlin,ZHOU Yiyan,YANG Lixin,et al.Shenzhen Blood Center, Shenzhen 518035,China;Detection of HBV DNA with full automatic system and HBV genotyping in blood donor screening[J];Chinese Journal of Blood Transfusion;2005-02
2 REN Furong1,WANG Jingxing2,ZHAO Haiyan1,NESS Paul3,SHAN Hua3,et al.1.Beijing Red Cross Blood Center,Beijing 10088,China;2.Institute of Blood Transfusion,Chinese Academy of Medical Sciences;3.Johns Hopkins Medical Institution,USA;Nucleic acid testing of human immunodeficiency virus and hepatitis C virus among blood donors from five cities of China: estimation of residual transfusion risk[J];Chinese Journal of Blood Transfusion;2007-06
3 WU Yuqing,YANG Zhongsi,ZHAO Lin,et al.Qingdao Blood Center,Qingdao 266071,China;Study of nuclei-acid-test(NAT) among volunteer blood donors from Qingdao area[J];Chinese Journal of Blood Transfusion;2008-11
4 ZENG Jinfeng1,XU Xiaoxun1,LI Huo1,CHEN Hua2△,et al.1.Shenzhen Blood Center,Shenzhen 518035,China;2.Shanghai Kehua Bioengineering Co.,Ltd.;Application of nucleic acid test in blood donor screening[J];Chinese Journal of Blood Transfusion;2011-07
5 WANG Wenbo1,XU Gang1,ZHA Zhanshan2,SUN Bo1,QIAN Baohua2.Co-corresponding author:REN Hao1,QI Zhongtian1.1.Department of Microbiology,Second Military Medical University,Shanghai 200433,China;2.The Blood station of Changhai Hospital;Establishment of rapid FQ-PCR screening method for HCV RNA in blood donors[J];Chinese Journal of Blood Transfusion;2012-04
6 YE Xian-lin1, ZENG Zhao-jian2,YANG Bao-cheng1,LU Liang1, SHANG Gui-fang1, LI Huo1(1.Shenzhen Blood Center, Shenzhen 518035,China; 2.Department of Transfusion Medicine,Dalian Medical University, Dalian 116025, China);A study on domestic nucleic acid amplification (PCR) reagent for HBV DNA in blood screening[J];Chinese Journal of Infection Control;2007-05
7 Liu Xia (Blood Center Station of Huaihua City,Huaihua,Hu′nan 418000,China);Infection status of HIV in blood donors without repayment in Huaihua Hu′nan[J];International Journal of Laboratory Medicine;2012-09
8 FENG Xiumei WANG Yan Jilin Red Cross Blood Center in Jinlin Province,Jilin 132001,China;Analysis of nucleic acid detection in screening blood donors[J];China Medicine and Pharmacy;2011-19
9 Jia Yin The Qinghai center of blood station;Detection and Analysis of HCV, HIV in the Collected Blood[J];Journal of High Altitude Medicine;2004-02
10 NI Hongying,OU Shanhai,XIE Jinzhen,CHEN Changrong.Xiamen Blood Center,Xiamen 361004,China;Application of single specimen nucleic acid test in donor blood screening[J];Chinese Journal of Blood Transfusion;2012-12
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Gao Feng,Zhang Xi,Wang Xun,et al.Shanghai Blood Center,Shanghai 200051,China;Investigation on the Mode of Double Testing of Blood for Virus Markers[J];Chinese Journal of Blood Transfusion;2001-04
2 Wang Xun,Zheng Lan,Zhang Xi,et al. Shanghai Blood Center, Shanghai 200051, China;Preliminary experiences of nucleic acid testing in blood screening in Shanghai[J];Chinese Journal of Blood Transfusion;2003-03
3 ZEN Jin-feng,LI Huo,XU Xiao-xun,et al. (The Clinical Laboratory of Shenzhen Blood Centre 518035,China);Analysis of Positive Blood Donors from NAT Screening:Comparison with ELISA Tests[J];International Journal of Laboratory Medicine;2010-06
4 ZHAO Quan-bi,CAI Shu-xia,LIU Yu-lei,et al.(State Key Laboratory for Infectious Diseases Prevention and Control,National Center for AIDS/STD Control and Prevention,China CDC,Beijing 102206 China);Nucleic acid testing (NAT) for HIV,HBV and HCV in the blood donors in Xinjiang·Yili·Kazak Autonomous Region[J];Chinese Journal of AIDS & STD;2010-06
5 HUANG Chenghui, CHEN Ruguang, HUANG Jianguo, et al. Shenzhen Bao An Blood Center, Shenzhen 518101, China;Nucleic acid amplification test of blood donations in blood bank[J];Chinese Journal of Laboratory Medicine;2001-03
6 REN Fu-rong*, LIU Chang-li, Lü Qiu-shuang, LI Hui, ZHANG Ping, GAO Dong-ying, GAO GUO-jing, MA Guo-dong, ZHAO Hai-yan, ZHUANG Hui *Beijing Red Cross Blood Center, Beijing 100088, China Corresponding author:ZHUANG Hui, Email:Zhuanghui@publia.bjcninfo.net;Nucleic acid testing in sero-negative blood donors[J];Chinese Journal of Laboratory Medicine;2004-09
7 CHENG Gang, HE Yunshao, ZHOU Xinyu. Da An Gene Diagnostic Center, Sun Yetsen University of Medical Science, Guangzhou 510089;Fluorescence quantitative PCR and its application in detection of hepatitis B virus[J];Chinese Journal of Laboratory Medicine;1999-03
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