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《International Journal of Laboratory Medicine》 2014-06
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PCR method established for the detection of aspergillus fumigatus

Cao Guojun;Xing Zhifang;Hua Li;Zhao Fang;Gu Wenhong;Pan Huifen;Zhao Zhen;Department of Clinical Laboratory,Shanghai Minhang District Central Hospital;Department of Blood Transfusion,Shanghai Minhang District Central Hospital;Shanghai Jiaotong University School of Public Health;  
Objective To establish a PCR method for the detection of aspergillus fumigatus.Methods Design a pair of special primers during the target sequences of aspergillus fumigatus mitochondrion gene.And the PCR reaction system was established by preliminary verification of its performances.Results The PCR method established had good sensitivity,stability and specificity with a detection linear range of from 102 to 108 copies/mL.Conclusion The PCR method established have good performances.But the clinical use of this method needs to be verified by more multi-center research.
【Fund】: 上海市闵行区科委自然科学基金资助(2011MHZ22 2013MHZ013)
【CateGory Index】: R346
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