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《International Journal of Digestive Diseases》 2008-06
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Effects of macrophage migration inhibitory factor on the proliferation and expression of angiogenesis factor in human colon carcinoma cell HT-29

LI Guo-qing,LIU Yan-ping,FENG Xia,et al.Department of Gastroenterology,the Second Affiliated Hospital of Nanhua University,Hengyang(421001),China  
Objectives To investigate the effects of macrophage migration inhibitory factor on the proliferation and expression of vascular endothelial growth factor(VEGF) and interleukin-8(IL-8) in colon carcinoma cell train HT-29.Methods The cultured HT-29 cells were treated with different concentrations of recombinant human macrophage migration inhibitory factor(rhMIF)(25,50,100μg/L) for different time.The proliferation of HT-29 cells was detected by MTT assay.The expression of VEGF and IL-8 mRNA in HT-29 cells were examined by real-time quantitative reverse transcription-polymerase chain reaction(RT-PCR) and the levels of VEGF and IL-8 protein in the supernatants were determined by enzyme-linked immunosorbent assay(ELISA).Results RhMIF promoted the proliferation of HT-29 cells in a concentration-and time-dependent manner(P 0.05),and the proliferating activity was increased with the raise of concentration and prolonging of action time.The expression of VEGF and IL-8 mRNA and the levels of VEGF and IL-8 protein in supernatants were increased gradually with the raise of concentration or action time(P0.05).Conclusions MIF promotes the proliferation of colon carcinoma cells,and MIF plays a crucial role in the angiogenesis of colon cancer and the roles of MIF may be realized via raising the expression of VEGF and IL-8.
【CateGory Index】: R735.35
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【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 Gan Jia Liang, Tang Zong Jiang, Gao Feng.The First Affiliated Hospital of GuangXi Medical University, Nanning 530021;The Relationship between Vascular Endothelial Growth Factor C Expression and Angiogenesis and Cell Proliferation in Colorectal Cancer[J];Journal of Coloproctological Surgery;2005-04
2 DAI Taiming,MA Hong,SONG Yufeng,FENG Hongchao,WEI Honglin(Department of Oral and Maxillofacial Surgery,The Affiliated hospital of Guiyang Medical College,Guiyang 550004,China);The Expression of Macrophage Migration Inhibitory Factor in Oral Squamous Cell Carcinoma and Its Significance[J];Journal of Guiyang Medical College;2007-04
3 Xu Yidong,Ren Yuanman,Lin Chunfang (Department of General Surgery,the Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,China);Tumor Necrosis Factor-alpha in Promotion of the Invasion of Human Gastric Carcinoma Cells by Activating Nuclear Factor-kappa B[J];Journal of Basic and Clinical Oncology;2008-03
4 WU Di,WANG Tian-zhen,HE Xiu-ping,et al (Department of Obstetrics and Gynecology,The First Clinical College, Harbin Medical University,Harbin 150001,China);Expression and clinical significance of MIF and Ezrin in ovarian cancer[J];Journal of Harbin Medical University;2009-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Fang Silian\;Sun Yat_Sen Memorial Hospital, Sun Yat_Sen University of Medical Sciences\;Wang Dazhang, Yang Xichuan,He Zhixiu,et al\;West China University of Medical Sciences\;;Morphological Observation of Buccal Mucosa Carcinoma Inhibited by Monoclonal Antibodies of Human Vascular Endothelial Growth Factor[J];West China Journal of Stomatology;2002-02
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