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Effects of macrophage migration inhibitory factor on the proliferation and expression of angiogenesis factor in human colon carcinoma cell HT-29

LI Guo-qing,LIU Yan-ping,FENG Xia,et al.Department of Gastroenterology,the Second Affiliated Hospital of Nanhua University,Hengyang(421001),China  
Objectives To investigate the effects of macrophage migration inhibitory factor on the proliferation and expression of vascular endothelial growth factor(VEGF) and interleukin-8(IL-8) in colon carcinoma cell train HT-29.Methods The cultured HT-29 cells were treated with different concentrations of recombinant human macrophage migration inhibitory factor(rhMIF)(25,50,100μg/L) for different time.The proliferation of HT-29 cells was detected by MTT assay.The expression of VEGF and IL-8 mRNA in HT-29 cells were examined by real-time quantitative reverse transcription-polymerase chain reaction(RT-PCR) and the levels of VEGF and IL-8 protein in the supernatants were determined by enzyme-linked immunosorbent assay(ELISA).Results RhMIF promoted the proliferation of HT-29 cells in a concentration-and time-dependent manner(P 0.05),and the proliferating activity was increased with the raise of concentration and prolonging of action time.The expression of VEGF and IL-8 mRNA and the levels of VEGF and IL-8 protein in supernatants were increased gradually with the raise of concentration or action time(P0.05).Conclusions MIF promotes the proliferation of colon carcinoma cells,and MIF plays a crucial role in the angiogenesis of colon cancer and the roles of MIF may be realized via raising the expression of VEGF and IL-8.
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