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《China Animal Husbandry & Veterinary Medicine》 2010-04
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The Expresstional Differences and Developmental Changes of bcl-2 Gene in Early Embryos of Quail

ZHOU Ling-ling,LI Yan,LIANG Du,LI Xing-ming,XU Cong-xiang,XIANG Chun-he,LIAO He-rong,HE Yan,(College of Life Science,Shihezi University,Shihezi 832003,China)  
The method of RT-PCR was used to multiply PCR to identify and clarify the quail embryo sex form 66 to 120 hatch hours with primers of Wpkci and β-actin,then selected four embryos of female and male respectively at each period,taken β-actin for internal standard to determine the relative quantity of embryo bcl-2 mRNA,to investigate the influences of apoptotic factors bcl-2 on early embryos of quail.The results showed that:① The bcl-2 mRNA expression of male embryos maintained lower level between 66 to 90 h of embryo stage,and it rose slowly between 96 to 102 h,the differences were not significant(P0.05);The bcl-2 mRNA expression of male embryos declined on 108 h(P0.05),but it rose distinctly on 114 h compare with 108 h(P0.05),then maintained higher level on 120 h.The bcl-2 mRNA expression of male embryos were higher than female embryos from 66 to 108 h(expect 72 h),but they were lower than female embryos from 114 to 120 h of embryo stage.② The bcl-2 mRNA expression of female embryos maintained lower level between 66 to 90 h of embryo stage;it rose distinctly on 96 h compare with 90 h(P0.05),and declined slowly from 96 to 108 h,the differences were no significant of them(P0.05),then declined distinctly between 114 to 120 h compare with 108 h(P0.05).③ On the stage of 84 h,the bcl-2 mRNA expression of female embryos were higher than male embryos,and the difference was significant(P0.05);The expression of bcl-2 mRNA in female embryos were higher than male embryos at 90 and 108 h of embryo stage(P0.01).But,the bcl-2 mRNA expression of male embryos were very higher than female embryos at 120 h of embryo stage(P0.01).To some extent,bcl-2 mRNA expression of female and male embryos in the embryonic development was sequential,indicated that the bcl-2 genes had an important effect on the development of the quail embryos.
【Fund】: 国家自然科学基金资助项目(30360071;30660125)
【CateGory Index】: S839
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