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《China Animal Husbandry & Veterinary Medicine》 2011-04
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Detection of Bovine Rotavirus by TaqMan Based Real-time Reverse Transcription Polymerase Chain Reaction Assay

FAN Qing,XIE Zhi-xun,LIU Jia-bo,PANG Yao-shan,DENG Xian-wen,XIE Zhi-qin,XIE Li-ji,PENG Yi(Guangxi Veterinary Research Institute,Nanning 530001,China)  
A TaqMan based real-time RT-PCR was developed for the specific detection of bovine rotavirus(BRV).The specific primers and probes were designed according to the VP6 sequences of BRV.It was found that the specificity of this assay was high without any cross-reactions and had no CSFV,BVD,MB and IBRV.A good linear correlation was demonstrated in the standard curve for the real-time PCR assay within the range of 102 to 109copies,with a correlation coefficient of 0.997.The detection limit of the real-time RT-PCR assay was 100 copies of viral RNA,indicating a good sensitivity of the assay.This TaqMan based real-time RT-PCR assay reported here is a valuable method with high specificity and sensitivity detection of BRV.
【Fund】: 国家百千万人才工程人选专项资金资助(945200603);; 广西科技攻关与新产品试制项目(桂科合0992033-5)
【CateGory Index】: S858.23
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