Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Animal Husbandry & Veterinary Medicine》 2012-06
Add to Favorite Get Latest Update

Identification and Culture of Neural Stem Cells Isolated from Postnatal Wistar Rat Hippocampus

CHEN Ke-yan,WANG Cheng-li,ZHANG He,AN Yang,SU Peng,SUN Qian,WANG Yang(Department of Laboratory Animal,General Hospital of Shenyang Military Area Command,Shenyang 110840,China)  
To establish a method to isolate culture and identify the neural cells from postnatal Wistar rat hippocampus.The neural stem cells were isolated from postnatal 24hWistar rat hippocampus and cultured with DMEM containing 20%fetal bovine serum / F12medium,and observation in inverted phase contrast microscope daily.The results showed that the neural stem cells isolated from postnatal Wistar rat hippocampus had reproductive activity and the exponential growth phase of neural stem cells was 2to 8dand maintain 30d.The cells were identified by immunofluorescence expression of Nestin positive,and immunohistochemistry results showed that the cell bodies in the subculture and processes were NF-positive markers,GFAP antibody and CD68antibody color were negative.Thus,the cells isolated and cultured from postnatal rat hippocampus had shown a strong ability of self-renewal proliferation and multipotency of differentiation which were evidently identified as the neural stem cells.
【Fund】: 辽宁省科技技术计划项目(2011408004)
【CateGory Index】: R329
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Li Kun, Guo Wanhua(Department of Histology and Embryology, Sun Yat-Sen University of Medical Sciences, Guangzhou);NEUROTROPHIC ACTIVITY IN BRAIN WOUND TISSUE EXTRACT AND THE ORIGIN OF THE ACTIVE FACTORS[J];Acta Anatomica Sinica;1991-02
2 Wang Tian-You, et al Department of Pathophysiology, Beijing Neurosurgical Institute, Beijing;“Ischemic damage” of cultured cerebral neurons[J];Chinese Journal of Pathophysiology;1989-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Lan1,ZHAO Qing1,HAN Zhi-xia2,QIU Zhi-qun1,CHANG Xiao-song1,CHEN Ji-an1,XU Chuan1,HU Ran1,SHU Wei-qun1,(1.Department of Environment Hygiene,The Third Military Medical University,Chongqing 400038,China;2.Department of Public Health,Luzhou Medical College,Luzhou 646000,Sichuan,China);Protective Effect of Transfection of recO Gene from Deinococcus Radiodurans on Oxidation and Inflammatory Injury of UVB-induced HSF in Vitro[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-02
2 PENG Chang-an et al(Department of Bioengineering,Wuhu Institute of Technology,Wuhu,Anhui 241006);Research on the Tumor Suppressor Role of Nostoc sphaeroides Polysaccharide Extracted by Microwave[J];Journal of Anhui Agricultural Sciences;2012-20
3 HUANG Hua1,CHEN Zhi-wen1,CHEN Zheng-xu2,HU Bo2,WANG Dong-ping2 (1.Department of Biochemistry and Molecular Biology,2.Research Center of Clinical Laboratory Science,Bengbu Medical College,Bengbu Anhui 233030,China);Inhibitory mechanism on the mice melanoma by arsenic trioxide[J];Journal of Bengbu Medical College;2008-04
4 Zhao Li,Qin Jianqiang,Chen Shiwen (Department of Anatomy,Bengbu Medical College,Anhui 233003 );Effect of macrophage conditioned media on the neurites outgrowth in ventral part of the spinal cord[J];JOURNAL OF BENGBU MEDICINAE COLLEGE;1998-06
5 GUO WAN-HUA(Department of Histology & Embryology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089)ZHOU MING-HUA (CHAU, R.M.W.)(Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong);THE EFFECT OF MACROPHAGE CONDITIONED MEDIUM ON THE SURVIVAL OF CEREBELLAR CORTICAL NEURON[J];Acta Zoologica Sinica;1992-04
6 Wang Wenhua,Zhang Huicheng Second Hospital Affiliated to Wenzhou Medical College(325003);The Influence of Qingdupian and Yangzhengpian on the Activity of u-PA in Leukemia Cells[J];Journal of Zhejiang University of Traditional Chinese Medicine;2009-02
7 Wang Wenhua1,Zhu Lili2,Shan Zesong3,et al 1.The Staff Room of Traditional Chinese Medicine,Wenzhou Medical College(325035),China;2.The Postgraduate Student of the Second Affiliated Hospital of Wenzhou Medical College;Effect of Hyodeoxycholic Acid on uPAR Expression of HepG2 Cells[J];Journal of Zhejiang Chinese Medical University;2012-01
8 CHEN Bo1,XIE Xi-mei1,JIA Ying2,LV Ming-zhuang2(1Guiyang College of TCM,Guiyang 550002,China;2Guiyang Medical College,Guiyang 550004,China);Study on synthesis and liberation of NO and IL-1β from fascia tissue cells of rat under pressure stimulation in vitro[J];China Journal of Traditional Chinese Medicine and Pharmacy;2008-07
9 XUE Ying 1,HUANG Jiong1,2 ,WANG Yu-hong2,ZHU Gang1,XA Pei-bei2,HE Sun2,LIU Hong2(1.Research Institute of Veterinary Science,Xinjiang Academy of Animal Science,Urumqi 830000,China; 2.Xinjiang Tiankang Animal Science Bio-Technology CO.LTD,Urumqi 830013,China);Influence of Different Medium and Density of Glucose to Vero Cell Growth[J];Grass-Feeding Livestock;2010-04
10 ;Free-serum culture of human bronchial epithelium obtained by bronchofiberscopic brushing[J];Academic Journal of Second Military Medical University;2004-07
China Proceedings of conference Full-text Database 6 Hits
1 WANG Wei-dong,HUANGHong,ZOU Hao-yuan,LIN Hong. Departmeent of Internal Medicine,Medical College of JinaYing University,Meizhou 510431,China;The influence of hypoxia-ischemia on the permeability of blood-brain barrier in vitro and its mechanism[A];[C];2010
2 Chen Bo~1 Luo Yongfen~2 Cui Jin~1 Feng Lingmei~1 Yong Xiaofang~1 Feng Lin~1 (1.Guiyang College of TCM,Guiyang 550002,China; 2.Chengdu University of TCM);A comparative study on the release of PGE_2 and IL-6 by taking static pressure on fibroblast cells gained from the rat“Zusanli” and its adjacent areas[A];[C];2007
3 An lilong,XiaoMei Zhang TuBao,Dong XiFang,Xu YingMei(college of Agriculture,GuangDong Ocean University,Zhanjiang Guangdong 524008,China);Effect of Different Concentration Neonatal Bovine Serum on the Growth and Proliferation of Primordial Germ Cells(PGCs) from Three-Yellow Broilers[A];[C];2008
4 Xiaomei,An lilong,Zhang TuBao,Wu LingFeng,GuoHongBo,Xu YingMei(college of Agriculture,GuangDong Ocean University,Zhanjiang Guangdong 524008,China);Research on Isolation and culture of pancreatic stem cells of mouse[A];[C];2008
5 WANG Yinan~(1,2),CONG Yi-mei~1,ZHANG Xin-miao~1,HU Kui~1,LIU Zhong-hua~(1△) (1 Laboratory of Embryo Biotechnology of Northeast Agricultural University,150030,Harbin,China;2 Songshan Lake Pearl Laboratory Animal Sci.& Tech.Co.,Ltd,Dongguan 523808);Development of isolation and culture of porcine neural stem cells[A];[C];2012
6 DIAO Ruixin,ZHANG Yong~*,HU Junjie,CHANG Weihua,LIN Tao,ZHANG Zhiqiang,XU Yanxia,HU Shaohui,ZHANG Lianmei,ZHAO Na (College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China);Establishment and characteristic of a Bactrian camel fetal ear marginal fibroblast cell line[A];[C];2012
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved