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《China Animal Husbandry & Veterinary Medicine》 2012-06
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Identification and Culture of Neural Stem Cells Isolated from Postnatal Wistar Rat Hippocampus

CHEN Ke-yan,WANG Cheng-li,ZHANG He,AN Yang,SU Peng,SUN Qian,WANG Yang(Department of Laboratory Animal,General Hospital of Shenyang Military Area Command,Shenyang 110840,China)  
To establish a method to isolate culture and identify the neural cells from postnatal Wistar rat hippocampus.The neural stem cells were isolated from postnatal 24hWistar rat hippocampus and cultured with DMEM containing 20%fetal bovine serum / F12medium,and observation in inverted phase contrast microscope daily.The results showed that the neural stem cells isolated from postnatal Wistar rat hippocampus had reproductive activity and the exponential growth phase of neural stem cells was 2to 8dand maintain 30d.The cells were identified by immunofluorescence expression of Nestin positive,and immunohistochemistry results showed that the cell bodies in the subculture and processes were NF-positive markers,GFAP antibody and CD68antibody color were negative.Thus,the cells isolated and cultured from postnatal rat hippocampus had shown a strong ability of self-renewal proliferation and multipotency of differentiation which were evidently identified as the neural stem cells.
【Fund】: 辽宁省科技技术计划项目(2011408004)
【CateGory Index】: R329
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