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《China Animal Husbandry & Veterinary Medicine》 2012-07
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PCR Amplification and Sequence Analysis of Partial 28S rRNA Sequence of three Eimeria tellena Strains

HOU Jie1,LIN Rui-qing1,LIU Li-dan2,CHEN Jun-jia1,WENG Ya-biao1(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;2.Foshan Standard Bio-Tech Co.Ltd.,Foshan 528138,China)  
The partial 28S ribosomal RNA sequences of three different Eimeria tellena strains isolated from Guangdong province were amplified by polymerase chain reaction(PCR),and compared with relevant sequences of E.tellena,E.acervulina,Sarcocystis muris,T.gondii available in GenBankTM.The results showed that the partial 28S rRNA sequence of three E.tellena strains was 1172 bp in length with no nucleotide difference in each other,and only one base difference found with E.tellena in GenBankTM,revealing highly conservative within E.tellena.There were distinct sequence differences found comparing with E.acervulina,S.muris and T.gondii,which demonstrated 28S rRNA could be used as a genetic marker for the population genetic studies on different species of Eimeria and other apicomplexan parasites.
【Fund】: 广东省自然科学基金(10451064201005443);; 广东省科技计划项目(2009B020307011)
【CateGory Index】: S852.72
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