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《China Animal Husbandry & Veterinary Medicine》 2013-04
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Cell Culture of Endometrial Epithelial Cells from Chinese Holstein Dairy Cows in vitro

ZHANG Shi-dong,WANG Dong-sheng,WANG Xu-rong,PAN Hu,LI Hong-sheng,YAN Zuo-ting(Key Laboratory of New Animal Drug Project of Gansu Province,Research Center of Clinical Veterinary Medicine of CAAS, Key Laboratory of Veterinary Pharmaceutics Discovery,Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS,Lanzhou 730050,China)  
To establish and perfect cell culture technique of endometrial epithelial cell(EnEpC)in dairy cows,primary cell was isolated and cultured by tissue cultivation that combined with enzymolysis of collagenase.The endometrial epithelial cell was purified from primary culture cells by different time digestion of trypsase,and EnEpC was identified by immunohistochemical detection of cytokeratin expression.The results demonstrated that flagstone-like epithelial cell was obtained by the adoptive method,and cytokeratin expression was positive in the cells.The purified cells could be subcultured about 10 times,and cells from generation 2 to 8 maintained stably biological characteristics of morphology and proliferation state that were found in primary culture cells.
【Fund】: 中央级公益性科研院所基本科研业务费专项资金项目(1610322012004);; “十二五”国家科技支撑计划重点课题(2012BAD12B03)
【CateGory Index】: S823
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Chinese Journal Full-text Database 5 Hits
1 ZHANG Yan,DU Wei-hua,HAO Hai-sheng,ZHAO Xue ming,WANG Dong, QIN Tong,LIU Yan,ZHU Hua-bin(Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Progress of Mammary Epithelial Cells Culture in vitro[J];China Animal Husbandry & Veterinary Medicine;2011-10
2 DONG Bin,SONG Yuxuan,ZHU Guangqin and CAO Binyun*(College of Animal Science and Technology,Northwest A&F University,Yangling Shaanxi 712100,China);Comparative Study of Method about Mouse Endometrial Epithelial Cell Culture[J];Acta Agriculturae Boreali-Occidentalis Sinica;2009-01
3 HE Zhi-quan,SHEN Wen-zheng,DOU Zhong-ying (Northwest A & F University,Shaanxi Branch of National Stem Cells Wngineering & Technology,Yangling,Shaanxi 712100,China);Isolation,culture and vitality examination of endometrium epidermal cells from pregnant mouse[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition);2006-02
4 Du Jinliang,Xu Lina,Ma Yuzhong,Qin Jianhua(College of Animal Science and Technology,Agricultural University of Hebei,Baoding Hebei 071001);Inflammatory Model of Endometrial Cells in Rabbits[J];Chinese Agricultural Science Bulletin;2009-04
5 WU Qing-xia1,2,WANG Ai-hua1,SI Yong-jia2,ZHOU Li-mei1,SHENG Hong-xia1,JIN Ya-ping1 (1.College of Veterinary Medicine,Northwest A &F University,Yangling,Shaanxi 712100,China;2.College of Animal Science,Tibet Agricultural and Animal Husbandry College,Linzhi,Tibet 860000,China);Immortalization of goat endometrial epithelial cells by telomerase reverse transcriptase transfection[J];Chinese Journal of Veterinary Science;2010-02
Chinese Journal Full-text Database 10 Hits
1 LIU Wei et al (Center of Animal Embryo Engineering,Qingdao Agricultural University,Qingdao,Shandong 266109);Isolation and Culture and Molecular Biological Identification on Cows Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2010-06
2 HE Zhi-quan et al(Department of Histology and Embryology,Zunyi Medical College,Zunyi,Guizhou 563000);Establishment of in vitro Co-culture to Mouse Parthenogenetic Embryos[J];Journal of Anhui Agricultural Sciences;2011-14
3 DING Qing1,YOU Zhao-ling1,TAN Zhen-yu2(1College of Combined Traditional Chinese and Western Medicine,Hunan University of TCM,Changsha 410208,China;2The fi rst Affi liated Hospital of Hunan University of TCM,Changsha 410007,China);Effect of active compound components of SQ on MMP-1,TIMP-1,VEGF,t-PA and PAI-1 of in? ammatory endometrial cell[J];China Journal of Traditional Chinese Medicine and Pharmacy;2009-03
4 YANG Qian,SHI Wan-yu,ZHAO Zhu-jun,ZHONG Xiu-hui(College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071001);Inflammatory model of endometrial cells in mice[J];Journal of Agricultural University of Hebei;2011-06
5 LI Junliang,WANG Jianliu,WANG Shijun,WEI Lihui.(Department of Obstetrics and Gynecology,Peking University People's Hospital,Beijing 100044,China);Effect of kangfuyan capsule's on rabbit model of pelvic inflammatory disease[J];Chinese Journal of Clinical Obstetrics and Gynecology;2010-06
6 LI Jun,MENG Xiang-ge,FAN Chao-qun (School of Life Science,Huaibei Coal Industry Teachers College,235000,Huaibei,Anhui,China);Endometrial Epithelium of Neonatal Mouse Separated in Vitro[J];Journal of Huaibei Coal Industry Teachers College(Natural Science Edition);2009-03
7 WANG Jia-li,GAO Xue-jun,LI Qing-zhang,HUANG Jian-guo,LU Li-min,LUO Chao-chao,LIU Rong,JIN Xin(The Key Laboratory of Dairy Science,Ministory of Education,Northeast Agricultural University,Harbin 150030,China);Influence of Methionine on Subcellular Proteins of Dairy Cow Mammary Gland Epithelial Cell[J];China Animal Husbandry & Veterinary Medicine;2012-04
8 ZHAO Shi-jun,GAO Yun-hang,LUAN Wei-min(College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin,130118,China);Progress on Improving Lactation of Dams by Traditional Chinese Medicine[J];Progress in Veterinary Medicine;2012-12
9 LI Xi-yan,WANG Jia-qi,WEI Hong-yang,BU Deng-pan,HU Han,ZHOU Ling-yun(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Bovine Mammary Epithelial Cells Culture Methods in vitro and Its Application[J];Acta Agriculturae Boreali-Sinica;2010-S2
10 ZHANG Lei, WANG Heng, QI Chang-ming,et al(Animal's medical college,CAU, Beijing 100094,China);Isolation,culture and identification of stromal and epithelial cells from endometrium of dairy cows[J];Heilongjiang Animal Science and Veterinary Medicine;2008-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Gao Yajun, Tang Shuang, Sun Sujun, Zhang YongInstitute of Bio-Engineering, Northwest A & F University, Yangling, Shanxi 712100, China;In Vitro Culture of Mammary Epithelial Cells in Goat[J];Anhui Agricultural Science Bulletin;2005-02
2 ZENG GUO-QING, CAO YU-JING (State Key Laboratpru of Reproductive Biology, Institute of Zoology, Academia Sinica, Beijing 100080);CO-CULTURE OF MOUSE EMBRYOS WITH UTERINE EPITHELIAL CELLS MONOLAYER[J];Acta Zoologica Sinica;1996-02
3 GE Zengguang, TONG Huili, QIN Jun, LI Qingzhang, GAO Xuejun (Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China);Effect of different Chinese herbs extracts on expression of β-casein of mouse mammary gland cells[J];Journal of Northeast Agricultural University;2009-01
4 TAN Dong-Mei 1, TAN Yi 1, ZHAO Jie 2, AI Ling 2, WANG Zhi- Biao 2 1Laboratory Animal Center, 2Ultrasound Engineering Institute of Medical Science, Chongqing University of Medical Science, Chongqing 400016, China;In vitro culture of mouse endometrium[J];Journal of The Fourth Military Medical University;2005-07
5 JIN Ya ping ,WANG Ai hua, WU Hao ,WANG Jian chen (College of Animal Science and Veterinary Medicine,Northwest Science and Technology University of Agriculture and Forestry,YangLing ,Shaanxi 712100,China);Preparation of Uterine intraepi the lial Lymphocytes in Mice and Goat[J];PROGRESS IN VETERINARY MEDICINE;2000-03
6 Lin Jing, Chen Yiquan (Department of Histology and Embryology,Fujian Medical University,Fuzhou,350004);Culture of Oviductal Epithelial Cells and Its Biological Characteristics[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;1997-02
7 CHEN Juan1,YANG Yong-mei1,CHEN Yang1,JIANG Huai-zhi1,YANG Yu-jiang1,GONG Jun-ming2(1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;2.The Animal Husbandry of Shuangliao,Shuangliao 136400,China);The Effects of Different Medium on the Culture of Mouse Ovarian Corpus Lutum Cells in vitro[J];China Animal Husbandry & Veterinary Medicine;2011-04
8 MA Fang, WANG Zhi-biao~△, WANG Yuan, ZHAO Jie. Department of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China;Comparative Research on the Implantation of Mouse Blastocyst in the Co-cultural System of Malignant Tumor Cells and Receptive Endometrium in vitro[J];Journal of Sichuan University(Medical Science Edition);2006-05
10 Zhong Yu; Zhang Chuxue; Pan Shanpei(Center for Reproductive Immunology Research, Jinan University ,Guangzhou 510632,China);Growth of Human Oviductal Epithelial Cells in Serum - free Medium[J];JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION);1998-S1
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