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《China Animal Husbandry & Veterinary Medicine》 2013-04
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Cell Culture of Endometrial Epithelial Cells from Chinese Holstein Dairy Cows in vitro

ZHANG Shi-dong,WANG Dong-sheng,WANG Xu-rong,PAN Hu,LI Hong-sheng,YAN Zuo-ting(Key Laboratory of New Animal Drug Project of Gansu Province,Research Center of Clinical Veterinary Medicine of CAAS, Key Laboratory of Veterinary Pharmaceutics Discovery,Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS,Lanzhou 730050,China)  
To establish and perfect cell culture technique of endometrial epithelial cell(EnEpC)in dairy cows,primary cell was isolated and cultured by tissue cultivation that combined with enzymolysis of collagenase.The endometrial epithelial cell was purified from primary culture cells by different time digestion of trypsase,and EnEpC was identified by immunohistochemical detection of cytokeratin expression.The results demonstrated that flagstone-like epithelial cell was obtained by the adoptive method,and cytokeratin expression was positive in the cells.The purified cells could be subcultured about 10 times,and cells from generation 2 to 8 maintained stably biological characteristics of morphology and proliferation state that were found in primary culture cells.
【Fund】: 中央级公益性科研院所基本科研业务费专项资金项目(1610322012004);; “十二五”国家科技支撑计划重点课题(2012BAD12B03)
【CateGory Index】: S823
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