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《China Animal Husbandry & Veterinary Medicine》 2013-08
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Inhibitory Effect of Cyperone on Inflammation of Chicken Alveolar Epithelial Type Ⅱ Cells Induced by APEC

ZHANG Li-yan,GUO Xun,XIA Fang,HU Xi-rou,QIN Qian-qian,ZHANG Cui,SONG Zhou, SHEN Hai-qing,YI Peng-fei,WEI Xu-bin,FU Ben-dong(College of Veterinary Medicine,Jilin University,Changchun 130062,China)  
The effects of cyperone on chicken alveolar epithelial type Ⅱ cells infected by APEC-O78 were evaluated through determining TNF-α,IL-10,TLR-4 and TLR-5 in vitro.The results showed that 0.5 μg/mL cyperone significantly decreased the secretion of TNF-α(P0.05).1 μg/mL cyperone extremly significantly increased IL-10 secretion(P0.01).Furthermore,0.1,0.5 and 1 μg/mL cyperones all extremly significantly decreased the expression of TLR-4 mRNA(P0.01),but had no effect on the expression of TLR-5 mRNA(P0.05).The results indicated that cyperone might inhibit the production of inflammatory cytokines of chicken alveolar epithelial type Ⅱ cells induced by APEC-O78 through blocking TLR-4 signaling pathway.
【Fund】: 吉林大学科学前沿与交叉学科创新项目(20120331);; 吉林大学博士科研启动基金项目(20070302)
【CateGory Index】: S858.31
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