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《China Animal Husbandry & Veterinary Medicine》 2013-09
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Expression of VP2 Gene of Infectious Bursal Disease Virus in Insect Cells

YANG Feng;LI Xian-wei;SUN Min-hua;YAN Fu-qiang;WEI Qing-lan;XIANG Bin;REN Tao;Key Laboratory of New Drug Discovery,Ministry of Agriculture,College of Veterinary Medicine, South China Agricultural University;  
The VP2 gene of very virulent infectious bursal disease virus( vvIBDV) JM-1 /10 strain was placed behind the CMV promoter of pcDNA-3. 1( +). The CMV-VP2 gene was cloned into the baculovirus expression system pFastBacTMDual to build pFastCMV-VP2. Transformed it to Escherichia coli DH10Bac. Recombinant baculovirus expression plasmid Bacmid-CMV-VP2 was screened. Bacmid-CMV-VP2 was used to be transfected into Sf9 insect cells to get the recombinant baculovirus vBac-CMV-VP2 and transduced into BHK-21 cells. After 48 to 72 hours,indirect immunofluorescence assay( IFA) was conducted to detect specific fluorescence. Western blotting analysis result showed target protein was expressed. The prepared recombinant vBac-CMV-VP2 could not only be expressed in insect cells,but also expressed in mammalian cells.
【Fund】: 省科技计划项目(2012A020800006)
【CateGory Index】: S852.65
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