Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Animal Husbandry & Veterinary Medicine》 2014-05
Add to Favorite Get Latest Update

Relations Between Bacterial Count and Absorbance of Streptococcus agalactiae in Bovine Mastitis

XIAO Min;YANG Feng;WANG Xu-rong;LUO Jin-yin;LI Xin-pu;JIA Ning;LI Hong-sheng;College of Veterinary Medicine,Gansu Agricultural University;Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS;  
The present study was aimed to establish a method for quickly and accurately calculating the concentration of Streptococcus agalactiae antigen in dairy cow mastitis vaccine.Streptococcus agalactiae were washed from blood plate using physiological saline and diluted into different concentrations of bacteria suspension,which were used to calculate the number of viable bacteria suspension by plate count method.Meanwhile the corresponding absorbance(A450nm)were determined.Then established the standard curve through the correlation between concentrations and absorbance.The result showed that the viable bacteria counts had a significant linear correlation with the absorbance of Streptococcus agalactiae.Thus,we build a regression equation between Streptococcus agalactiae bacteria suspension concentration and absorbance was y=7.5861x-0.0805,R2= 0.9735,through which we could quickly calculate Streptococcus agalactiae concentration by measure the absorbance of its suspension.Compared to traditional plate count method,the absorbance measurement accuracy of the method was verified better by 5batches of Streptococcus agalactiae bacteria suspension fostered by fermentor,and proved the new method has characteristics of simple,fast and accurate.
【Fund】: “十二五”国家科技支撑计划(2012BAD12B03);; 甘肃省农业生物技术项目(GNSW-2008-03)
【CateGory Index】: S858.23
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 8 Hits
1 YANG Hui-ping, ZHANG ZHou, XIAO Nai-zhi(Yangling vocational technical college Yanglin Shaanxi 712100);Research progression of dairy cow Subclinic mastitis[J];Journal Yellow Cattle Science;2005-02
2 LUO Tai-Lai1, SHEN Liang-Liang1, BAI Yin-Lan2, XUE Ying21Team 1, Department of Pharmacy, 2Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China;Comparative study of Mycobacterium counting methods[J];Journal of the Fourth Military Medical University;2007-04
3 LI Hong-sheng, YU Jie, LI Xin-pu, LUO Jin-yin, XU Ji-ying, ZHANG Li-hua,PAN Hu, ZHANG Zhi-chang,YUAN Yong-long, YANG Yu-ying, LIU Chun-chuan, HOU Yi-zhao (Institute of Lanzhou Animal Science and Vet. Pharmaceutics,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730050,China);Study on Relativity between Mastitis Type and Pathogenic Bacterial Infection in Dairy Cows[J];Progress In Veterinary Medicine;2004-06
4 LI Hong-sheng1,LUO Jin-yin1,WANG Xu-rong1,LI Xin-pu1,YANG Feng1,YU Jie2,XU Ji-ying3,YAN Zuo-ting1(1.Research Center of Clinical Veterinary Medicine of CAAS,Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province,Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture,Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;2.Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300,China; 3.Northwest University for Nationalities,Lanzhou 730000,China);Research of the Effects of Different Adjuvants Multiple Vaccines for Cow Mastitis Immunization on Mice[J];China Animal Husbandry & Veterinary Medicine;2013-01
5 TAO Hai-jiang and WANG Lao-qi (Biological Engineering Department of Zhengzhou College of Animal Husbandry & Engineering, Zhengzhou, Henan 450011, China);A study on bacterial counts in preparing an own vaccine against fowl Escherichia coli[J];Journal of Zhengzhou College of Animal Husbandry Engineering;2003-03
6 YAN Pei-feng,ZHANG Kong-hai,LI Jian-fang(Department of Food Science,Xinyang Agricultural College,Xinyang 464000,China);On the relations between viable bacteria counts and absorbency of fermented liquid of lactobacillus[J];Journal of Xinyang Agricultural College;2012-01
7 LI Hong-sheng,YU Jie,LI Xin-pu,LUO Jin-yin,XU Ji-ying,ZHANG Li-hua,GUO Fu-cun,HAN Fu-jie,YANG Yu-ying(Lanzhou Institute of Animal Science and Veterinary Pharmaceuticals,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China);Development of inactivated multi-vaccine against dairy mastitis and observation of its clinical efficacy[J];Veterinary Science in China;2007-04
8 Yuan Yunglong Zhang Lihua Liu Chunchuan Yang Yuying Hou Yizhao Li Hongsheng Pan Hu Zhang Zhichang Yu Jie Li Xinpu (Institute of Traditional Chinese Veterinary, Chinese Academy of Agricultural Sciences Lanzhou 730050 Zhang Yongxin (Institute of Gongbei Animal and Plant Quarantine Zhuhai 519020;A SURVEY OF THE PATHOGENS OF BOVINE MASTITIS IN CHINA[J];Scientia Agricultura Sinica;1992-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Xiao-feng et al(Sygen Biotechnology Center,Helan,Ningxia 750200);Effect Observation of Chinese Herb on the Dairy Cow Ulcer Hoof Disease[J];Journal of Anhui Agricultural Sciences;2006-05
2 QU Dong-mei et al (School of Chemical and Material Engineering,Jiang Nan University,Wuxi,Jiangsu,214122);Research on the Extraction Tchmology of Antibacterial Substance From Rhubarb with Ultrasonic Assisted Extraction Method[J];Journal of Anhui Agricultural Sciences;2009-01
3 DENG Hai-ping et al(Lanzhou Institute of Animal Sciences and Veterinary Pharmaceutics,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu 730030);Separation and Identification on Pathogens from Cow Mastitis in Inner Mongolia Area and Their Drug Sensitive Experiment[J];Journal of Anhui Agricultural Sciences;2009-02
4 WANG Zuo-xin et al(Department of Veterinary Medicine of Gansu Agricultural University,Lanzhou,Gansu 730070);Study on Antibacterial Activity of Rubia cordifolia L.on the Major Pathogenic Bacteria Causing Mastitis[J];Journal of Anhui Agricultural Sciences;2009-02
5 TIAN Hai-yan et al(Lanzhou Institute of Husbandry and Animal Pharmaceutics,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu 730050);Studies on Screening Medium for Streptococcus agalactiae of Cow Mastitis and Its Influential Factors[J];Journal of Anhui Agricultural Sciences;2009-08
6 ZHANG Jie~1,LE Wei~2,CHEN Jia-kuan~2(1.College of Life Science,Zhongnan Division,Wuhan University of Science and Technology,Wuhan 430223;2.Wuhan Huizhi Science and Technology development co. Ltd.);Serologic Evaluation of Capsular Polysaccharide of Staphylococcus aureus from Bovine Mammary Isolates in China[J];Amino Acids & Biotic Resources;2006-04
7 ZHANG Jie(College of Biological & Environmental Science,Zhejiang Wanli University,Ningbo 315100,China);Development of Indirect ELISA for Detecting Cow Mastitis Vaccine[J];Amino Acids & Biotic Resources;2009-03
8 Li Cungui;Herbal Prevention Research Cow Mastitis[J];Beijing Agriculture;2011-12
9 Li Cungui;Herbal Prevention Research Cow Mastitis[J];Beijing Agriculture;2011-21
10 WU Guo-Juan, ZHANG Zhong-Wen et al (Beijing Agricultural College,Beijing 102206);Antibacterial Effects of ChineseHerbal Medicines on Six Pathogenic Bacteriafrom Dairy Cows Mastitis[J];Journal of Beijing Agricultural College;2003-03
China Proceedings of conference Full-text Database 4 Hits
1 ZHOU Ye,YANG Liang-yu,WANG Hao-ran YANG Yu-ai (College of Animal Science and Technology,Yunnan Agricultural University,Kunming, Yunnan,650201);Isolation,identification & drug sensitivity test of the pathogenic bacteria of cow mastitis in Kunming area[A];[C];2011
2 FU Yan-ling LIU Ai-ling LI Xu-dong (Ringpu(Tianjin)Bio-Pharmacy Co.,Ltd.Tianjin,300300;China);Advance of the Prevention and Cure of Dairy Cow Mastitis[A];[C];2008
3 Yang Feng Li~(a,b),He Bao Xiang~(a,b,*),Li Xiao Shan~b,Yang Xian Ling~b, Du Yu Lan~b,Li Gong He~(a,b),Huang Qin Hua~(a,b),Liu Ping~(a,b) a Institute of Animal Reproduction,Guangxi Key Laboratory for Subtropical Bio-Resource Conservation and Utilization,Guangxi University,Nanning,Guangxi 530005,China b Animal Science and Technology College,Guangxi University,Nanning,Guangxi 530005,China;Study of Incidence,Etiology and Malonaldehyde Level and Some Enzymatic Activities of Subclinical Cows Mastitis in Subtropical China[A];[C];2010
4 Dong shu-wei,Liu Yong-ming~*,Qi Zhi-ming,Li Xia,Liu Shi-xiang,Wang Sheng-yi (Lanzhou Institue of Animal Sciences & Veterinary Pharmaceutics of CAAS,Gansu Lanzhou 730050,China);Effect observation of the self-prescribed chinese herbal compound on treating diarrhea in calves[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Hong-sheng,YU Jie,LI Xin-pu,LUO Jin-yin,XU Ji-ying,GUO Fu-cun,ZHANG Li-hua (Lanzhou Institute of Animal and Veterinary Pharmaceutics Science, CAAS,Lanzhou,Gansu,730050,China);Inducing Acute Mastitis in Milk Goats by Using Three Common Pathogenic Bacteria Isolated from Dairy Mastitis[J];Progress In Veterinary Medicine;2004-04
2 SHEN Ke-fei1,CAO Lan2,YIN Ji-gang1,JIANG Ning1,CHEN Qi-jun1(1.Institute of Zoonosis,Jilin University and Key Laboratory of Zoonosis,Ministry of Education,Changchun,Jilin,130062,China;2.Center of Diagnosis,Veterinary Research Institute of Chongqing,Chongqing,402460,China);Progress in Adjuvants[J];Progress in Veterinary Medicine;2007-S1
3 LI Xiu-ping1, YIN Xun-he2,GAO Chen1, YIN Yan-bo3,WU Shi-you3(1.Agricutural Department of Dezhou University,Dezhou,Shandong,253015;2.College of Animal Technology,Shandong Agricultural University, Taian,Shandong,271018,China;3.Shangdong Oland Bio-engineering Institute,Qingdao,Shandong,266101,China);Uiversal Gene Chip for Detection of the Pathogenic Bacteria of Cow Mastitis[J];Progress in Veterinary Medicine;2008-06
4 WANG Ru1,XIE Yin-qian2,SHEN Zhi-qiang2,MIAO Xiao-qing1(1.Fujian Bees Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China;2.Institute of Animal Husbandry and Veterinary Medicine,Binzhou Academy of Shandong,Binzhou 256618,China);The Immunological Enhancement and Application of Propolis[J];China Animal Husbandry & Veterinary Medicine;2007-02
5 DAI Ding-zhen~1,YANG Yong-gui~2,FANG Tai-hui~3,WANG Xiao-li~1,XIA Xing-xia~1,JIANG Zhao-chun~1,HU Yuan-liang~2 (1.Institute of Veterinary,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.Veterinary college,Nanjing Agricultural University,Nanjing 210095,China;3.Department of Pharmacology,Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China);Antibacterial Action in vitro and Inhibition Effect on Streptococcus agalactiae LDH of Chinese Herbal Medicine[J];Jiangsu Journal of Agricultural Sciences;2006-01
6 TAO Hai-jiang and WANG Lao-qi (Biological Engineering Department of Zhengzhou College of Animal Husbandry & Engineering, Zhengzhou, Henan 450011, China);A study on bacterial counts in preparing an own vaccine against fowl Escherichia coli[J];Journal of Zhengzhou College of Animal Husbandry Engineering;2003-03
7 XUE Mao-yun1,DAI Ding-zhen2,CHEN Zhong-ming2,ZHANG Zhi-cheng2, DU Gai-mei2 (1.Jiangsu Institute of Economic and Trade Technology,Nanjing 210007,China; 2.Jinling Institute of Technology,Nanjing 210085,China);Detction of Staph. aureus in Milk By Polymerse Chain Reaction(PCR)[J];Journal of Jinling Institute of Technology;2007-03
8 SHUANG Jin, Gaerdi, BAO Peng-yun, Wuyundalai, QI Zhi-li (College of Aminal Science and Medicine,Inner Mongolia Agricultural University,Huhhot 010018,China);OCCURANCE REGULARITY OF DAIRY CATTLE`S SUBCLINIC MASTITIS SEPARATING PATHOGENES AND MEDICINE SENSITIZATION TEST[J];Journal of Inner Mongola Institute of Agriculture and Animal Husbandry;2001-01
9 XIE Ji zhi , XIAO Hong bin(Agricultural College of Yangzhou University,Yangzhou 225009,China);Inspection and evaluation of lactic acid bacteria and acidity on yoghurt[J];China Dairy Industry;2002-01
10 Jia Zhishen WuJianmin Tang Mengcheng(Teaching Group of Inorganic and Analytic Chemistry,ZhejiangAgricultural University,Hangzhou 310029,China).;Colorimetric Determination of Hydroxyl Radicals from Fenton Reaction[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1996-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved