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《Guangdong Trace Elements Science》 2006-10
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The Level of 25-dihydroxy Vitamin D_3 and Effects of 1α,25-dihydroxy Vitamin D_3 on IL-4、IFN-γ Production in Hashmoto's Disease

WANG Donggang,XIONG Feng,LEI Peiyun(Childeren's Hospital,Chongqing University of Medical Sciences,Chongqing 400014,China)  
To analyze the effects of 1α,25-dihydroxy vitamin D_3 on interleukin-4(IL-4),interferon-gamma(IFN-γ)from Th1/Th2 at the level of T cell directly in children with hashmoto's thyroiditis(HT)in vitro and to analyze the level of serum 25(OH)D_3,and provide with theory basis in therapy for HT,human peripheral blood monocytes(PBMCs) from 27 HT patients and 17 healthy children isolated from heparinized peripheral blood by density gradient centrifugation with Ficoll-Paque were stimulated to active the T cell by phytohaemagglutinin(PHA),then cultured under the condition in the presence or absence of 1×10~(-8) mol/L(1α,25(OH)_2D_3) respectively.After 72 h,collected the culture supernatant in sterility.IL-4,IFN-γ were measured by enzyme-linked immunosorbent assay(ELISA)in the culture supernatant.Meanwhile serum was gotten from anotner part of the peripheral blood,then 25(OH)D_3 was extracted and estimated by radioimmunoassay(RIA).The results showed the level of 25(OH)D_3 decreased strongly compared with healthy controls in HT,(21.85±5.73) ng/mL vs(27.56±7.46) ng/mL(P0.01) respectively,and IFN-γ significantly increased compared with healthy controls,(2 146.15±355.01) pg/mL vs(1 462.00±101.52)pg/mL(P0.01) respectively,while IL-4 decreased significantly,(52.26±7.17)pg/ml vs(59.32±4.21)pg/mL(P0.01) respectively.And most important,in the presence of 1α,25(OH)_2D_3,IFN-γ was dramatically down-regulated in HT(1 536.00±243.95)pg/mL(P0.01),whereas IL-4 levels were not significantly different,(53.15±5.75)pg/mL(P0.05),so they had a lower IFN-γ/IL-4 ratio in response to(1α,25(OH)_2 D_3.)It concludes 1α,25(OH)_2D_3 could inhibit the increased Th1 cytokines IFN-γ in HT at T cell level,improve disbalance of Th1/Th2 cytokines of HT.Therefore,1α,25(OH)_2D_3 could be used in immunotherapy for HT.
【Fund】: 重庆市科委资助项目
【CateGory Index】: R725.8
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【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 FANG Yi, LIU Jian-feng, GUO Qing-ling,et al. Department of Nuclear Medicine, The 307th Hospital Affiliated to Academy of Military Medical Sciences, Beijing 100039, China;The Diagnosis of Hashimoto Thyroiditis: A Report of 45 Cases[J];Journal of Chinese Physician;2005-03
2 WANG Donggang,XIONG Feng,LEI Peiyun(Children's Hospital,Chongqing University of Medical Sciences,Chongqing 400014,China);Regulation of 1 Alpha,25-dihydroxy Vitamin D_3 to IL-10、IL-12 in Children with Hashmoto's Disease[J];Guangdong Trace Elements Science;2006-11
3 WANG Zhibin (Department of Otolaryngology, Tongji Hospital,Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030);Diagnosis and treatment in 45 patients with Hashimoto's thyroiditis associated with throat symptoms[J];Journal of Clinical Otorhinolaryngology;2003-02
4 WANG Xi-dong 1,CHI Bao-en 2,ZHANG Zhen-xing 2,LI Li-ning 1,DU Jin-lang 2 (1.Department of General Surgery,Inner Mongolia Hospital,Hohhot 010017,China 2.Department of General Surgery,First Affiliated Hospital,Inner Mongolia Medical College,Hohhot 0100;THE ADVANCE OF CLINICAL STUDY IN HASHIMOTO THYROIDITIS AND SUBACUTE THYROIDITIS[J];Acta Academiae Medicinae Neimongol;2002-01
5 XU Shaoming, WANG Ping, YU Zhigang. Second Affiliated Hospital, Zhejiang University, Hangzhou 310009, China;Clinical research of Hashimoto′s disease with thyroid carcinoma[J];NATIONAL MEDICAL JOURNAL OF CHINA;2000-04
6 Wang Donggang,Xiong Feng,Lei Peiyun.Childeren's Hospital,Chongqing University of Medical Sciences,Chongqing 400014,China;Effects of vitamin D_ 3 on T_H1/T_H2 cytokines production in children with Hashmoto's disease[J];Chinese Journal of Practical Pediatrics;2006-12
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 Wang Donggang,Xiong Feng,Lei Peiyun.Childeren's Hospital,Chongqing University of Medical Sciences,Chongqing 400014,China;Effects of vitamin D_ 3 on T_H1/T_H2 cytokines production in children with Hashmoto's disease[J];Chinese Journal of Practical Pediatrics;2006-12
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