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《World Phytomedicines》 2008-03
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Analysis and identification different parts from Acanthopanax senticosus by Fourier Transform Infrared Spectroscopy

JIN Zhe-xiong XU Sheng-yan Pharmaceutical College,Harbin University of Commercial,Harbin 150076,China SUN Su-qin Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology,Department of Chemistry,Tsinghua University,Beijing 100084,China  
Objective To analyse the multi-steps IR macro-fingerprint of raw radix,stem,leaf,and total glycoside extract of Acanthopanax senticosus.Methods By Multi-steps IR macro-fingerprint Method.Results The spectra of raw medicinal materials show that the spectra of radix and stem are similar and mainly show characteristic peaks of calcium oxalate and starch,whereas the characteristic peaks of calcium oxalate of leaf almost disappears and its shape of characteristic peak of starch also gets unconspicuous.FT-IR spectra of total glycoside extract of radix,stem,and leaf present characteristic peaks at 1 602,1 514,1 452(vibration of phenyl framework)and 1271 cm~(-1)(=C-O),therefore,their mutual component is the compound of phenolic glycoside.Through observing second derivative IR spectra of total glycoside extract of different parts of Acanthopanax senticosus Harms,the characteristic peak of leaf is stronger than that of radix and stem at 1656 cm~(-1)(flavone C=O),it proves the quantity of component of flavone in the leaf is higher than that in the radix and stem.In two-dimensional correlation spectra,the radix and stem both have five automatic peaks(vibration of phenyl framework)at 1350—1700 cm~(-1),whereas the leaf still shows another automatic peak at 1656 cm~(-1)(flavone C=O),it further proves the quantity of component of flavone in the leaf is higher than that in the radix and stem.Thus it can be seen IR macro-fingerprint technique can provide plentiful holistie information and hold general quality of Acanthopanasx senticosus Harms.Conclusion The multi-steps IR macro-fingerprint method was demonstrated to be a rapid,simple,and reliable method on the quality control of Traditional Chinese Medicine.
【Fund】: 科技部“十五”重大科技攻关、“重大技术标准研究”专项“食品中药与天然产物有效成分检测技术研究”课题(2002BA906A29-4)
【CateGory Index】: R284
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