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《Journal of Chemical Engineering of Chinese Universities》 2002-06
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Continuous Fermentation of Ganoderma lucidum Exo-polysaccharide in a Membrane Bioreactor

ZHANG Xue-hong1, HU Hong-bo1, TANG Yong-lian1, HUANG Rui-shan1, CHEN Zheng-jie2 (1. School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China; 2. Zhejiang Hisun Pharmaceutical Co. Ltd, Taizhou 318000, China)  
It was observed that the concentration of exo-polysaccharide (EPS) produced by Ganoderma lucidum fermentation decreased during stationary phase in batch culture. In this work, a membrane bioreactor system was applied in the fermentation of G. lucidum in order to decrease the loss of EPS. In the system, EPS and G. lucidum mycelia were separated by micro-pore membrane according to there size, mycelia were recycled back into fermentor and could reach a high density, and in the same time exo-polysaccharide secreted continuously. On the other hand, the effluent of membrane apparatus containing EPS was harvested and fresh media were added to the bioreactor to maintain constant volume. With this system, the loss and degradation of EPS could be well controlled. The yield of exo-polysaccharide and growth of G. lucidum mycelia were investigated at different dilution rates ranging from 0 to 0.24 h-1. Although the concentration of EPS in the effluent in continuous fermentation was a little lower than that in batch fermentation and decreased with the increase of dilution rate, the weight of mycelia increased 20% and the total amount of harvested EPS increased by 500% comparing with those in batch culture. Moreover, the process was beneficial for EPS separation by the effluent broth without mycelia. The result showed that it was an effective process to produce EPS in a membrane bioreactor system, and perhaps, can be applied for other fungi to produce EPSs in high yield.
【CateGory Index】: TQ929.2
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