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《Journal of Chemical Engineering of Chinese Universities》 2004-02
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Application of Electrodialysis Desalination to the Separation of Glutamine from Fermentation Broth

LIU Yuan-shuai, SHEN Jin-yu, XING Xin-hui, YU Li-xin, LI Chun, CAO Zhu-an (Department of Chemical Engineering, Tsinghua University, Beijing 100084, China)  
A new technique of one-step anion exchange for purification of glutamine from its fermentation broth was developed by using electrodialysis desalination to remove (NH4)2SO4 existing in the broth. Adjusting the pH of broth to the the isoelectric point of glutamine, most (NH4)2SO4 and only a little glutamine would transfer from the broth to the adjacent chamber through ion membrane during electrodialysis operation. When the desalination ratio reached 95%, the recovery ratio of glutamine and the current efficiency were 76% and 85%, respectively. After electrodialysis desalination, glutamine could be separated successfully from glutamic acid by passing the retained broth through a single anion exchange column. Experimental results show that this new process is effective in decreasing the amount of resins, preventing the decomposing of glutamine in strong acid and alkaline environment, and in the reduction of the production cost. The total yield of glutamine reaches about 60% and the purity of product satisfies the pharmaceutical standard.
【Fund】: “十五”国家科技攻关项目;;项目编号2001BA708B0205。
【CateGory Index】: TQ464
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