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《Journal of Chemical Engineering of Chinese Universities》 2007-01
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Laccase Production by Coriolus versicolor Fermentation and its Decolorization Effect on Indigo Bule Dye

GAO En-li, ZHANG Shu-jiang, XIA Li-ming (Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China)  
The main technological parameters of the laccase production by Coriolus versicolor in submerged fermentation were studied. The corncob residue, a lignocellulosic waste from xylose manufactory, was found to be an effective carbon source for laccase production and the suitable corncob residue concentration to be used is 20.0 g?L?1. The addition of 10.0 g?L?1 glucose into culture medium could promote the growth of mycelium in the prophase and benefit the laccase synthesis during the anaphase. The most suitable C/N ratio of culture medium is 20 and adding appropriate amounts of Cu2+ (0.10 mmol?L?1) and ascorbic acid (1.50 mmol?L?1) could obviously enhance the laccase production. The experiments of producing laccase by Coriolus versicolor in a 3.7 L fermentor show that, with the above suitable condition and the most appropriate aeration rate of 100 L?h?1, after 144 h the activity of the produced laccase can reach 2474.2 IU?mL?1. The simulating decolorization experiments of the waste water from printing and dyeing mill were conducted, in which the colored water containing 50 mg?L?1 indigo blue was used as the simulated waste water and the degradation agent used is crude laccase solution. The results show that the decolorization efficiency of the produced laccase can reach 94.8% when the enzyme dosage of 0.45% (V/V) and the reaction time of 40 min were used. It shows that the produced laccase has a good application prospect in both biochemical treatment of dyeing effluent and biofinishing of blue jeans.
【CateGory Index】: X703
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