Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Optics and Precision Engineering》 2005-06
Add to Favorite Get Latest Update

Two-laser scanning confocal system for microarray analysis

MA Jun-shan,HOU Lin-lin,FU Dong-xiang,CHEN Jia-bi,ZHUANG Song-lin(Optics and Electronics Information Institute,Shanghai University of Science and Technology,Shanghai 200093,China)  
A dual-fluorescence microarray scanner based on laser confocal principle was constructed to detect the Cy_3-protein or Cy_5-protein dotted slides. The images were constructed according to the fluorescent intensity signals,and enhanced through adopting the technique of morphologic methods to meet the situation in which the fluorescent intensity was very low and the dynamic range of the data was wide relatively.The measuring ways of the signal-to-noise ratio and the sensitivity of the system were put forward,and the measurement result shows the sensitivity of the system is about 0.1 fluo/μm~2.
【Fund】: 上海市科委科技发展基金资助(No.022261016)
【CateGory Index】: TN495
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 7 Hits
1 QIAO Xiao-yan1,3,LI Gang1,HE Bing-jun2,LIN Ling1(1.College of Precision Instruments & Opto-electronics Engineering,Tianjin University,Tianjin 300072,China;2.College of Life Science and Technology,Nankai University,Tianjin 300071,China;3.College of Physics & Electronics Engineering,Shanxi University,Taiyuan 030006,China);Effects of low power laser irradiation on characteristics of transient outward potassium current of neurons[J];Optics and Precision Engineering;2007-03
2 ZHAO Ming-fu1,2,LIAO Qiang1,CHEN Yan2,ZHONG Nian-bing2(1.Institute of Engineering Thermophysics,Chongqing University,Chongqing 400044,China; 2.Department of Electronic Engineering,Chongqing Institute of Technology, Chongqing 400050,China);Fiber sensor for biomass' online testing[J];Optics and Precision Engineering;2007-04
3 CAI Jian-wen,LI Ping-ping,XU Chuan-ming,HUANG Wen-hao(Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China,Hefei 230027,China);Study on solar cell testing system and its parameter matching optimization[J];Optics and Precision Engineering;2007-04
4 LIU Rui-an1,2,JIN Shi-jiu1,WU Xiao-rong2,ZHANG Xi-kun2 (1.State Key Laboratory of Precision Measuring Technology and Instruments,Tianjin University,Tianjin 300072,China;2.College of Physics and Electronic Information Science,Tianjin Normal University,Tianjin 300074,China);Adaptive regulation of CCD camera in eye gaze tracking system[J];Optics and Precision Engineering;2007-06
5 LUO Yi1,WANG Xiao-dong1,YANG Fan2,LIU Chong2 (1.Key Laboratory of Precision & Non-traditional Machining,Ministry of Education,Dalian University of Technology,Dalian 116023,China;2.Key Laboratory of Micro/Nano Technology and System of Liaoning Province,Dalin University of Technology,Dalian 116023,China);Variable temperature quasi-creep experiment for fabrication of microfluidic chip using Cyclo-olefin Polymer(COP)[J];Optics and Precision Engineering;2007-07
6 LIU Yong1,CHEN Jia-bi2(1.Department of Physics,Zhaoqing University,Zhaoqing526061,China;2.College of Opto-electro-information Engineering,Shanghai University of Science & Technology,Shanghai200093,China);A confocal endoscope for tomographic imaging of tissue[J];Optics and Precision Engineering;2008-04
7 YANG Bin,LIU Le,LIU Zhi-yi,MA Sui-hua,CHONG Xin-yuan,HE Yong-hong(Laboratory of Optical Imaging and Sensing,Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China);Line-scanning quasi-confocal fluorescence imaging[J];Optics and Precision Engineering;2010-05
Chinese Journal Full-text Database 10 Hits
1 AO Lin 1 ,ZENG Zhi-xiong 2 ,FANG Zhi-jun 2 ,HU Ran 1 , GAO Li-hong 1 ,YANG Meng-shu 2 ,CAO Jia 1, (1.Department of Toxicology,College of Preventive Medicine,Third Military Medical University,Chongqing400038,China; 2.Applied Research Center for Genomic Technology,City University of Hong Kong,Hong Kong,China);The Design and Fabrication of Mouse Toxicology Gene Chip[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-02
2 ZHAO Li-xin,HU Song,YAN Wei,LUO Zheng-quan(Institute of Optics and Electronics,Chinese Academy of Sciences,Chengdu 610209,China);Technique of conjugate focus imaging and the using in biochip detecting[J];Micronanoelectronic Technology;2004-04
3 YU Yong1,2,HU Song1 (1.Institute of Optics and Electronics,Chinese Academy of Sciences,Chengdu 610209,China; 2.Graduate School of Chinese Academy of Sciences,Beijing 100039,China);Laser Confocal Biochip Scanner Based on PMT[J];Micronanoelectronic Technology;2006-01
4 LU Han-kui, JI Hong, ZHU Rui-sen, et al. (Department of Nuclear Medicine, Shanghai Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China);Comparative Study Between Bio-chip Detection and Chemiluminescent Immunoassay for Multi-tumor Markers[J];Labeled Immunoassays and Clinical Medicine;2005-02
5 LU Han-kui, JI Hong, GU Jian-ying, et al. (Department of Nuclear Medicine, Shanghai Sixth People's Hospital of Shanghai Jiaotong University, Shanghai 200233, China);Preliminary Clinical Application of Bio-chip Detection for Multi-tumor Markers[J];Labeled Immunoassays and Clinical Medicine;2005-04
6 WEI Qing-juan,YAN Yong-nan,KONG Bo,QIN Yu-hua (Chemical Engineering College of Northeast Dianli University,Jilin City,Jilin Province,China,132012);Application and Development Trend of Micro-array Technology[J];Journal of Northeast Dianli University(Natural Science Edition);2008-06
7 ;Surface modification of glass slides by monoclonal antibody for protein microarray fabrication[J];Acta Academiae Medicinae Militaris Tertiae;2005-08
8 WANG Yuan-lun, TANG Yu-de, TAO Kai-hua,LI Yue-xi,JIN Hui-ying,ZHANG Jin-hai(The Military Medical Institute,Nanjing Commander Area,Nanjing,Jiangsu,210002,China);Detecting Animal Infection Source of Leptospirosis By Genechip Technique[J];Progress In Veterinary Medicine;2005-02
9 ZHANG Deng-ke,NI Xu-xiang,SHI Yan ( National Centre of Engineering & Technology Research of Optical Instrument, State Key Lab of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, China );Image processing in suspension biochip detection[J];Opto-Electronic Engineering;2006-06
10 HE Xian-min*, HE Jia , WU Cheng XIANG Zhao-ying, et al . *Department of Health Statistics, Second Military Medical University, Shanghai 200433, China ;Microarray Core Facility Weill Medical College of Cornell University NEW YORK USA;Classification methods used for microarray data analysis[J];;2004-03
China Proceedings of conference Full-text Database 3 Hits
1 HOU Lin-lin, MA Jun-shan, FU Dong-xiang (Institute of Optics and Electronics Information, Shanghai University of Science and Technology, Shanghai 200093,China);De-noising technology for biochip image restoration[A];[C];2005
2 Li Jia Chen Liangge Lin Huashui Tian Zhaowu State Key Laboratory for Physical Chemistry of Solid Surfaces and Department of Chemistry Xiamen University, Xiamen,361005;Fabricating Method for Microarrayers[A];[C];2005
3 You Xueyi Zheng Xiangjun Li Dan Tianjin University,Tianjin,300072;Stability of Plane Poiseuille Flow in Microchannels[A];[C];2005
Chinese Journal Full-text Database 10 Hits
1 TUO Youkang,CHEN Ximing (Chongqing University of Posts and Telecommunications, Chongqing 400065, P.R.China);Half-wave loss when light waves are reflected at interface of two mediums[J];Journal of Chongqing University of Posts and Telecommunications;2004-06
2 ;Infrared Optical Fiber Sensor for the Measurement of Solution Concentration[J];Sensor World;2004-10
3 Li Reifeng Zhao Mingfu (Chongqing Management College of Industry,Chongqing,400050);A NEW PHOTOELECTRIC LIQUID-LEVEL SENSOR[J];COLLEGE PHYSICAL EXPERIMENT;1999-03
4 Miao Jianxun;Li Shuiqian;Shi Fawang; Cao Wanmin; Wu Jinlou (Luoyang Institute of Technology,Luoyang,471039);STUDY ON THE BASIC PROPERTIES OF THE SOLAR CELL[J];PHYSICAL EXPERIMENT OF COLLEGE;1996-04
5 KANG Zong-ming,ZHANG Li,XIE Pan(Department of Electronic Engineering,Tsinghua University,Beijing 100084,China);Implementation of an Automatic Focusing Algorithm Based on Spatial High Frequency Energy and Entropy[J];Acta Electronica Sinica;2003-04
6 REN Si-gang, LI Jian-wei, XIE Li-li (Institute of Optoelectronic Engineering, Chongqing University, Chongqing 400044, China);Automatic focusing technique based on gray scale difference method[J];Opto-electronic Engineering;2003-02
7 ZHOU Yong_jun1, CHEN De_qiang1, HUANG Wen_hao1, XIA An_dong1,2(1.Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230026,China;2. Molecular Reaction Dynamics Lab, Institute of Chemistry, Chinese Academy of Sciences,Beijing 100080,China);Development of the scanning system for confocal laser scanning fluorescence microscope[J];Optics and Precision Engineering;2002-06
8 CUI Dun jie (State Key Lab of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130022, China );How to define noise and associated performance parameters for infrared detector and infrared focal plane array detectors[J];Optics and Precision Engineering;2003-03
9 ZHAO Hui, BAO Ge-tang, TAO Wei (Department of Instrumentation Engineering, Shanghai Jiao Tong University, Shanghai 200030,China);Experimental research and analysis of automatic focusing function for imaging measurement[J];Optics and Precision Engineering;2004-05
10 ZHU Zheng-tao~(1,2),LI Shao-fa~1,CHEN Hua-ping~2 (1.College of Computer Science and Engineering, South China Univ. of Tech., Guangzhou 510640,China;2.College of Information Engineering, Guangdong Univ. of Tech., Guangzhou 510640,China);Research on auto-focused function based on the image entropy[J];Optics and Precision Engineering;2004-05
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 HE Yong,FU Jian-zhong,CHEN Zi-chen (Department of Mechanical Engineering,Microsystem Research and Development Center,Zhejiang University,Hangzhou 310027,China);Study on polymer flow profile in micro hot embossing[J];Optics and Precision Engineering;2008-02
2 ZHOU Chun-lan,WANG Wen-jing,LI Hai-ling,ZHAO Lei,DIAO Hong-wei (Institute of Electrical Engineering,Chinese Academy of Sciences,Beijing 100080,China);Characterization of crystalline silicon solar cells by electrical parameters[J];Optics and Precision Engineering;2008-07
3 LI Ling,Yang Li-jun,WANG Yang,LIU Bei,WANG Zhe (School of Mechatronics Engineering,Harbin Institute of Technology,Harbin 150001,China);Laser and water-jet fiber coupling technology for water-jet guided laser micromachining[J];Optics and Precision Engineering;2008-09
4 LIN Ling1,JIA Fang-rong1,LI Gang1,CHENG Li-jun1,QIAO Xiao-yan2(1.State Key Laboratory of Precision Measurement Technology and Instruments,Tianjin University,Tianjin 300072,China;2.College of Physics and Electronic Engineering,Shanxi University,Taiyuan 030006,China);Effect of pulsating magnetic fields on neuron transient outward potassium channel[J];Optics and Precision Engineering;2008-09
5 ZHUANG Xu-ye1,2,WU Yi-hui1,WANG Shu-rong1, XUAN Ming1(1.State Key Laboratory of Applied Optics,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100039,China);Optical fiber evanescent field sensor based on new type D-shaped fiber[J];Optics and Precision Engineering;2008-10
6 DAI Ming1,LING Li-qing1,2,SUN Li-na1,LI Yong-jun1,2(1.Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100039,China);Full-automatic light adjustment control for CCD camera by external trigger[J];Optics and Precision Engineering;2008-11
7 LIU Chong1,2,LIANG Yong1,LI Jing-min1,LIU Jun-shan1,WANG Xiao-dong1,2(1.Key Laboratory for Micro/ Nano Technology and System of Liaoning Province,Dalian 116023,China;2.Key Laboratory for Precision and Non-traditional Machining Technology of the Ministry of Education,Dalian University of Technology,Dalian 116023,China);Design of collision protection system of handling robot for microfluidic chip[J];Optics and Precision Engineering;2009-01
8 ZHANG Yun-hai,ZHAO Gai-na,ZHANG Zhong-hua,GU Yi-ming(Suzhou 66 Vision-Tech Co.,Ltd.,Suzhou 215005,China);Accurate focusing of non-mydriatic fundus camera[J];Optics and Precision Engineering;2009-05
9 LIU Yong-hong1,2,ZHAO Zhan2,PANG Cheng1,2,FANG Zhen1(1.State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100190,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100039,China);Dielectrophoretic traps and micro-motor for driving and sorting latex spheres:from discrete devices to a lab-on-a-chip[J];Optics and Precision Engineering;2009-06
10 TAO Lin2,LUO Yi1,2,ZHANG Yan-guo2,ZHANG Zong-bo2,WANG Xiao-dong1,2(1.Key Laboratory for Precision and Non-traditional Machining Technology of the Ministry of Education,Dalian University of Technology,Dalian 116023,China;2. Key Laboratory for Micro/Nano Technology and System of Liaoning Province,Dalian 116023,China);Fabrication of micro energy director on polymethyl methacrylate substrate for micro ultrasonic bonding[J];Optics and Precision Engineering;2009-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved