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《Guangxi Sciences》 2007-04
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The Extraction of Peroxidase from Momordica charantia and Its Property

LIU Jin-lei1,SU Tao2,LI Dian-peng1,LU Feng-lai1(1.Guangxi Institute of Botany,Guilin,Guangxi,541006,China;2.Guilin Yibai Lijiang Pharmaceutical Co.Ltd,Guilin,Guangxi,541006,China)  
Peroxidase was separated and purified from Momordica charantia.Its optimum pH value,optimum temperature,thermal stability and effect of substrate concentration on enzyme activity were determined.The results were showed as follows:With O-phenylenediamine as substrate,λmax=425nm,Km=4.6×103,Vmax=0.137unit/s,optimum pH value 4.6,optimum temperature at 50℃ with high enzyme activity.The inactivation rate of POD raised over 60℃,and the enzyme activity was almost lost above 70℃.Vitamin C and Cysteine had great inhabitation effect on POD activity,the inhibition rate reached 94%and 92% respectively.Al3+ was weaker,with inhibition rate of 69%.EDTA had no significant effect of inhibition,the inhibition rate was only 13%.
【CateGory Index】: Q946
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 HE Ping1,SHI Wei ping1,FU Xue lin2,HUANG Zhuo lie1(1.Life Science College;2.Agricultural College, South China Agric. Univ., Guangzhou 510642,China);The Purification of Peroxidase from Sugarcane Seedling and its Characteristic[J];Journal of Shanghai Jiaotong University (Agricultural Science);2003-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SUN Yu-jun~(1,2),CHEN Yan~1,WANG Xun~1,LI Yan-fei~1 (1.School of Life Science,AnHui University,Hefei 230039,China;2.Department of Applied Biology,Anhui Technical Teacher's College,Fengyang 233100,China);Studies on the isolation,purifications and characterization of an extracellular polysaccharide from armillaria mellea[J];Journal of Anhui University(Natural Sciences);2005-05
2 YE Sheng-mei1,2(1.Dept.of Biolo.,Anhui Normal University,Wuhu 241000 China;2.Dept.of Bioch.Engn.,Anhui University of Technology and Science,Wuhu 241000 China);Pretretment of the rice straw[J];Journal of Anhui University of Technology and Science(Natural Science);2008-04
3 Hou Xindong~1 Sheng Guilian~1 Zhang Hua~2 et al(1 College of Environment,China University of Geosciences,Wuhan 430074;2 Agronomy College,Xinjiang Agricultural University,Wulumuqi 830052);A Research for Methods of Extracting Plasmid DNA[J];Anhui Agricultural Science Bulletin;2006-13
4 MA Lei et al (College of Animal Science and Veterinary Medicine, Northwest Science and Technology University of Agriculture and Forestry, Yangling, Shaanxi 712100);Construction of E.coli-B.subtilis Expressional Shuttle Vector (pYG43) and Secretion of B.subtilis Alkaline Cellulase[J];Journal of Anhui Agricultural Sciences;2005-03
5 WANG Yun et al (College of Biological Science & Technology,Yangzhou University,Yangzhou,Jiangsu 225009);Preliminary Study on Chitosan as a Hydrophobic Interaction Chromatographic Matrix[J];Journal of Anhui Agricultural Sciences;2005-07
6 WANG Yun et al(School of Biological & Environmental Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013);Study on Chitosan-immobilized Trypsin[J];Journal of Anhui Agricultural Sciences;2005-11
7 LIU Ya-li(College of Life Science,Henan Normal University,Xinxiang,Henan 453007);Effect of Methanol and Vc on the Decrepitude of Vicia faba[J];Journal of Anhui Agricultural Sciences;2006-04
8 CHEN Yan(College of Life Science and Technology,Yancheng Teachers College,Yancheng,Jiangsu 224002);Effects of Different pH values on the DNA Extraction Rate[J];Journal of Anhui Agricultural Sciences;2008-05
9 WU Xiao-yong et al(Laboratory for Chemistry of Traditional Chinese Medicine,Chengdu University,Chengdu,Sichuan 610106);Grade Separation of Cellulase from Snailase[J];Journal of Anhui Agricultural Sciences;2009-04
10 ZHAO Ke-qin et al(Department of Bio-chemical Engineering,Hunan Electrical and Mechanical Vocational and Technical College,Changsha,Hunan 410151);Research on the Bacterial Cellulose Production with Soybean[J];Journal of Anhui Agricultural Sciences;2009-08
China Proceedings of conference Full-text Database 3 Hits
1 CHEN Ming SUN Changkui CHENG Jianfeng WANG Ximeng ZHANG Yuzhen (State Key Laboratory of Micro- biotechnology, Shandong University, Ji 'nan 250100, China;Research on Chitinase L15-2 and Its Inhibitory Action on Glomerella cingulata[A];[C];2005
2 GAI Dong-liang,ZOU Xiao,LIU Ai-ying,ZHAO Jie-hong (Institute of Fungual Resources of Guizhou University, Guiyang 550025, China);Study on the Mutagenic Breeding of the Strains of Monascus purpureus[A];[C];2006
3 S.P.Li,P.Li China Pharmaceutical University,Nanjing 210009;Nucleic Acid,Active Principles in Traditional Chinese Medicine and Gene Therapy[A];[C];1999
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 LUO Mao ZOU Guo lin WU Kai lang (College of Life Sciences,Wuhan University,HuBei, Wuhan,430072 China);Studies on the Purification of Peroxidase from Banboo shell and Its Applications[J];Amino Acids & Biotic Resources;2000-04
2 Huang Zhuolie Pan Tao (College of Biotechnology, South China Agric.Univ., Guangzhou, 510642);COMPARATIVE STUDIES OF PEROXIDASE ACTIVITIES AND ISOENZYMES IN SIX VARIETIES OF SUGARCANE[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1998-04
3 Liu Wen Fang Jing Gao Pei Ji (State Key Laboratory of Microbial Technology,Shandong University,Jinan 250100);Purification and Characterization of Soybean Hull Peroxidase[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1998-05
4 TIAN Guo Zhong 1, LI Huai Fang 2, QIU Wei Fan 2(1.Research Institute of Forest Environment and Protection,Chinese Academy of Forestry, Beijing 100091, China; 2.College of Plant Science and Technology, China Agricultural University, Beijing 100;Advances on Research of Plant Peroxidases[J];Journal of Wuhan Botanical Research;2001-04
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