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《Genomics and Applied Biology》 2010-02
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Preliminary Study on the Activities of Protease in Digestive Juice of Pitcher Plant

Tang Libo Ji Keping Wang Yan Chen Jingyong Department of Biochemistry ZhuhaiCampus Zunyi Medical College,Zhuhai,519041  
In order to analyze the character of the protein of the digestive juice in the ascidium of pitcher plant,we carried out a study of effect in different temperature and pH on activity of protease in digestive juice of pitcher plant we also compared 3 kinds of precipitation methods of protease concentrate and isolated protein sample in digestive juice by using SDS-PAGE. The results showed that the optimum temperature of protease in digestive juice in the ascidium was 50℃ with the maxlmum stability,and the peak value of activity of protease was found with the pH5. Experimental results indicated the best method of the protein concentrate was chloroform/butanol (5:1),and there were at least three kinds of protein component with different MW,i.e. 24.3 kD,35.1 kD and 61.4 kD,respectively,it was also shown that the protein with 35.1kD has the character of antitrypsine. This research should supply some evidences for the exploitation and utilization reasonable in wild resource of pitcher plant.
【Fund】: 遵义医学院校区启动基金(KY200603)资助
【CateGory Index】: Q946
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