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《Genomics and Applied Biology》 2010-02
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Preliminary Study on the Activities of Protease in Digestive Juice of Pitcher Plant

Tang Libo Ji Keping Wang Yan Chen Jingyong Department of Biochemistry ZhuhaiCampus Zunyi Medical College,Zhuhai,519041  
In order to analyze the character of the protein of the digestive juice in the ascidium of pitcher plant,we carried out a study of effect in different temperature and pH on activity of protease in digestive juice of pitcher plant we also compared 3 kinds of precipitation methods of protease concentrate and isolated protein sample in digestive juice by using SDS-PAGE. The results showed that the optimum temperature of protease in digestive juice in the ascidium was 50℃ with the maxlmum stability,and the peak value of activity of protease was found with the pH5. Experimental results indicated the best method of the protein concentrate was chloroform/butanol (5:1),and there were at least three kinds of protein component with different MW,i.e. 24.3 kD,35.1 kD and 61.4 kD,respectively,it was also shown that the protein with 35.1kD has the character of antitrypsine. This research should supply some evidences for the exploitation and utilization reasonable in wild resource of pitcher plant.
【Fund】: 遵义医学院校区启动基金(KY200603)资助
【CateGory Index】: Q946
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【Citations】
Chinese Journal Full-text Database 8 Hits
1 YU Hua(Department of Bioengineering,Chengdu University,Chengdu 610106,China);Study on in Four Methods in Comparison Removing of Protein from Polysaccharide of Laminaria Japonica Aresch[J];Journal of Chengdu University (Natural Science);2005-04
2 WU Tian, YE Chang hui, ZHANG Xiu zhi[HJ2/3] ( Agronomy College, Zhanjiang Ocean University , Zhanjiang 524088, China ) [WT5”HZ];Studies on the anatomical structures of the leaf of Nepenthes mirabilis (Lour.) Druce[J];GUIHAIA;2000-02
3 XU Chimo(Information Center,CATAS,Danzhou,Hainan571737);A Review in Research of Nepenthes[J];Chinese Journal of Tropical Agriculture;2003-05
4 ;纳豆激酶的提取研究[J];Science and Technology of Food Industry;2007-01
5 Rongjing Zhang1,2,Fuwu Xing 1,Laiping Siu3,Yan Liu4,Yushi Ye1,Saichit Ng 3,Hongfeng Chen1,Faguo Wang 1 1 South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650 2 Graduate University of the Chinese Academy of Sciences,Beijing 100049 3 Kadoorie Farm & Botanic Garden,Hongkong 4 Guangxi Institute of Botany,Guilin 541006;Spermatophyte flora of Yinggeling Mountain,Hainan[J];Biodiversity Science;2007-04
6 YU Lan, YU Xiang-an. The State Food and Drug Administration of the Second Affiliated Hospital of Lanzhou Medical College, Gansu 730000, China;Trichloroacetic acid precipitation plus biuret colorimetry to determine total protein in hirudo extract[J];Chinese Remedies & Clinics;2004-09
7 Yang Guangsui1, Huang Shaohua1, Xu Shisong1, Gao Yanmei2 (1Tropical Crops Genetic Resources Institute, CATAS, Hainan Danzhou 571737; 2Tropical Agricultur University of South China, Hainan Danzhou 571737);The Investigation and Study on the Germplasm Resources and Growth of the wild Common Nepenthes in Hainan[J];Chinese Agricultural Science Bulletin;2006-11
8 WANG Ying,ZHANG Li-ping(College of Food Science,Hei Long Jiang August First Land Reclamation University,Daqing 163319);Progress in separation and purification technology of pepsin[J];China Food Additives;2008-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SONG Hui et al(Department of Food Engineering,College of Xuzhou,Xuzhou,Jiangsu 221008);Extraction and Preliminary Purification of Soluble Dietary Fiber from Arctium lappa L[J];Journal of Anhui Agricultural Sciences;2009-05
2 PU Yue-wu et al(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou,Guangdong 510006);Study on Antioxidative Activities of Maca Polysaccharides[J];Journal of Anhui Agricultural Sciences;2009-28
3 LI Dong-mei,TAN Feng-zhi,YANG Jing-feng,YIN Hong-ling,LI Tao,ZHU Bei-wei(College of Biology and Food Technology,Dalian Polytechnic University,Dalian 116034,China);Isolation,Purification and Identification of Polysaccharides from Abalone Viscera[J];Fisheries Science;2007-09
4 YANG Li-rong,YANG Xiao-bo,LI Dong-hai,HUANG Yun-feng,LUO Tao,Guo Tao(Key Laboratory of Tropical Horticulture Resources and Genetic Improvement in Hainan University ofMinistry of Education,Haikou 570228,Hainan,China);Classification and Ordination of Secondary Forests of Zhayun Twonship in the Interior Mountainous Area of Hainan[J];Journal of Fujian Forestry Science and Technology;2010-03
5 SUN Tong-Xing1,JIANG Xing-Shan2(1.School of Life Science and Technology,Yancheng Teachers College,Yancheng 224002,China;2.College of Life Science,South China Agricultural University,Guangzhou 510642,China);H_2O_2-CH_2COOH maceration——a simple and perfect method for leaf epidermis[J];Guihaia;2009-01
6 ZHENG Ai-chu,CHEN Jian,PENG Chao-ying(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China);Extraction and Purification of Acidic Mucopolysaccharide from Holothuria scabra Jaeger[J];Modern Food Science and Technology;2007-05
7 ZHANG Rong-jing1,2,CHEN Hong-feng2,YE Yu-shi2,NG Sai-chit3,4,XING Fu-wu2,WANG Fa-guo2 ( 1 College of Life Sciences,South China Agricultural University,Guangzhou 510642,China; 2 South China Botanical Garden,The Chinese Academy of Sciences,Guangzhou 510650,China; 3 Kadoorie Farm & Botanic Garden,Hongkong; 4 Division of Technology,Beijing Normal University-HongKong Baptist University,Zhuhai,519085,China);Spermatophyte Resources of Yinggeling Mountain,Hainan[J];Journal of South China Agricultural University;2010-03
8 CHEN Shu-hua,ZHANG Shu-ping,DU Xiu-xiu,ZHOU Qin-sheng(University of Shanghai for Science and Technology,shanghai 200093,PRC);Methods of Deproteinization and Decoloration for Fucoidan[J];Hunan Agricultural Sciences;2011-07
9 JIA Qi②,LI Xue-Hua(②Department of Pharmacy,Guangxi Medical University,Nanning 530021,Guangxi China);The Structural Analysis of the Polysaccharide of Arillus Longan[J];Pharmaceutical Journal of Chinese People's Liberation Army;2009-01
10 YUAN Lu,GONG Jiang,YANG Mu,HHYAN Han-rong,XU Yao,LI Xiao-hui(Jilin Academy of Animal Science and Veterinary Medicine,Changchun Jilin 130062);Extraction Techniques and Methods of Polysaccharides[J];Jilin Animal Husbandry and Veterinary Medicine;2011-02
China Proceedings of conference Full-text Database 2 Hits
1 YANG He-sheng~(1,2) YANG Xiu-zhen~1 XU Heng~2 (~1 School of landscape Architecture,Beijing Forestry University,Beijing 100083; ~2 Biology Deparment Jiaying University,Meizhou 514015);Effect of Three Phytohormones Induction on the Cutting of Nepenthes hybrida[A];[C];2007
2 BI wei,XING yanyi LEI haimin,LI qiang,GONG wei-hong (Beijing University of Traditional Chinese Medicine,Beijing 100102,China);Determination of peptide in Carapax trionycis by visible spectrophotography[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WT4”BZ] ZHANG Yan wen, WANG Hai yang ( School of Life Sciences, Wuhan University , Wuhan 430072, China );Advances in the studies on insectivorous plants[J];GUIHAIA;2000-01
2 WU Tian, YE Chang hui, ZHANG Xiu zhi[HJ2/3] ( Agronomy College, Zhanjiang Ocean University , Zhanjiang 524088, China ) [WT5”HZ];Studies on the anatomical structures of the leaf of Nepenthes mirabilis (Lour.) Druce[J];GUIHAIA;2000-02
3 ZHU Hua, LI Yan-hui, XU Zai-fu, WANG Hong, LI Bao-gui [WT8.5BZ]( Xishuangbanna Tropical Botanical Garden, The Chinese Academy of Sciences, Kunming, 650204, China ) [WT9.5HZ];Characteristics and affinity of the flora of Xishuangbanna,SW China[J];Guihaia;2001-02
4 DING Tan 1,2 , LIAO Wen-bo1, JIN Jian-hua1, WANG Bo-sun1 ( 1.School of Life Science, Zhongshan University, Guangzhou 510275, China; 2.Guangzhou Institute of Forestry Science, Guangzhou 510515, China );Floristic analysis on the seed plants of Mt. Diaoluo in Hainan Island[J];Guihaia;2002-04
5 Su Zhiyao (College of Forestry, South China Agricultural University )Chang Hungta (Department of Biology, Zhongshan University );GENERA OF GUANGXI'S FLORA: AN AREAL TYPE ANALYSIS[J];GUIHAIA;1994-01
6 ZHONG Qiong_xing, LIU Qiang, TANG Cheng_xiong, ZHONG Yi (Institute of Tropic Biotic Resources,Hainan Teachers' College,Hainan Haikou,571158,China);The resources of medicinal plants on the islands around Hainan Island and the exploitation[J];Guizhou Science;2002-03
7 ZHANG Ji-ping,GUO Zhao-liang,TANG Dong-sheng,CHEN Jian-chou,HE Shun-lian (Department of Animal Science,Foshan Science and Technology College,Foshan 528231,PRC);Purification and Property of Stomach Proteinase from Fugu obscurus[J];Journal of Hunan Agricultural University;2004-06
8 Li Fuchuan, Tang Zhihong, Cui Bowen, Xi Zhenle, Wang Hairen (Deparment of Biology, Shandong University, Jinan 250100);HYPOGLYCEMIC ACTIVITY OF THREE POLYSACCHARIDES FROM LAMINARIA JAPONICA[J];CHINESE JOURNAL OF MARINE DRUGS;2000-05
9 ZHU De-yan,et al. (Jingmen Vocational Technical College,Jingmen,Hubei 448000);The Separation of Nattokinase in Leavening Agent[J];Journal of Jingmen Vocational Technical College;2002-03
10 XIAO Mei-yan, Xu Er-ni,GUO Xiao-yam,WU Gui-sheng(The Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China);SELECTION ON THE IMMOBILIZINGMATERIALS OF BACILLUS NATTO CELLS[J];Journal of Nanchang University(Natural Science);2005-04
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